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Circulating Exosomal MicroRNA-21 as a Biomarker in Each Tumor Stage of Colorectal Cancer
Open accessAbstract
Objective: We clarified the predictive and prognostic value of circulating plasma exosomal microRNA-21 (miR-21) in each TNM stage of colorectal cancer (CRC) patients. Methods: The microRNA (miRNA) profiles of the plasma exosomes, primary tumor tissues, and liver metastasis tissues from the same CRC patients were examined using a microarray. For validation analysis, the plasma exosome samples from 326 CRC patients were measured by TaqMan miRNA assays. Results: In the miRNA microarray analyses, miR-21 showed the highest upregulation in exosomes, primary tumor tissues, and liver metastasis tissues. Significant correlations were demonstrated between exosomal miR-21 and tissue miR-21 levels. As for the relationship to the pathological condition, exosomal miR-21 showed a significant association with liver metastasis and TNM stage. The overall survival (OS) rates and disease-free survival (DFS) rates in high-exosomal-miR-21 patients were significantly worse than those in low-miR-21 patients. Exosomal miR-21 levels were an independent prognostic factor for OS and DFS in CRC patients with TNM stage II or III, and for OS in patients with TNM stage IV. Conclusion: Plasma exosomal miR-21 levels are a useful biomarker for the prediction of recurrence and poor prognosis in CRC patients with TNM stage II, III, or IV.
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“Moreover, miR-21 has been demonstrated to be packaged within exosomes and released from different cancer types (51–54).”
From Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma (2019) · cites this paper as [53]
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Metadata
- Authors
- Tsukamoto, M., Iinuma, H., Yagi, T., Matsuda, K., & Hashiguchi, Y.
- Affiliations
- Teikyo University (JP)
- Venue
- Oncology
- Publisher
- S. Karger AG
- Published
- 2017
- Volume
- 92
- Issue
- 6
- Pages
- 360-370
- ISSN
- 0030-2414 (ISSN-L)
- 1423-0232
- OpenAlex
- W2605003516
- Cited by
- 203
- References
- 36
- Citations by year
- 2026: 8 · 2025: 10 · 2024: 11 · 2023: 26 · 2022: 22 · 2021: 42 · 2020: 31 · 2019: 27 · 2018: 20 · 2017: 6
- FWCI
- 9.39
- Type
- journal article
- Language
- en
- Open access
- Licence
- Locations
- Oncology (publishedVersion)
- PubMed (publishedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion, cc-by-nc-nd)
- Funders
- Japan Society for the Promotion of Science (17K10608, 15K10150, 17K10655)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Circular RNAs in diseases
- Keywords
- colorectal cancer, circulating exosomes, plasma biomarkers, TNM stage, liver metastasis, prognostic factors, overall survival, disease-free survival, tumor recurrence prediction
- MeSH terms
- Humans, Liver Neoplasms, Neoplasm Recurrence, Local, Neoplasm Staging, Prognosis, Tumor Cells, Cultured, Biomarkers, Tumor, Colorectal Neoplasms, Gene Expression Regulation, Neoplastic, Survival Rate, MicroRNAs, Microarray Analysis
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