Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
Abstract
Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.
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Many studies have detected extracellular/circulating miRNAs in biological fluids such as plasma, serum, cerebrospinal fluid, saliva, breast milk, urine, tears, colostrum, peritoneal fluid, bronchial lavage, seminal fluid, and ovarian follicular fluid.
“Many studies have detected extracellular/circulating miRNAs in biological fluids, such as plasma and serum (117, 118), cerebrospinal fluid (119), saliva (120), breast milk (121), urine, tears, colostrum, peritoneal fluid, bronchial lavage, seminal fluid (122), and ovarian follicular fluid (123).”
From Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation (2018) · cites this paper as [117]
Extracellular miRNAs are highly stable, resisting degradation at room temperature for up to 4 days and in deleterious conditions such as boiling, multiple freeze-thaw cycles, and high or low pH.
“Contrary to cellular RNA species, extracellular miRNAs are highly stable, resisting degradation at room temperature for up to 4 days and in deleterious conditions such as boiling, multiple freeze-thaw cycles, and high or low pH (117, 124).”
From Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation (2018) · cites this paper as [117]
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- Authors
- Chen, X., Ba, Y., Ma, L., Cai, X., Yin, Y., Wang, K., Guo, J., Zhang, Y., Chen, J., Guo, X., Li, Q., Li, X., Wang, W., Zhang, Y., Wang, J., Jiang, X., Xiang, Y., Xu, C., Zheng, P., Zhang, J., Li, R., Zhang, H., Shang, X., Gong, T., Ning, G., Wang, J., Zen, K., Zhang, J., & Zhang, C.-Y.
- Affiliations
- State Key Laboratory of Pharmaceutical Biotechnology (CN)
- Tianjin Medical University Cancer Institute and Hospital (CN)
- BGI Group (China) (CN)
- Shanghai Jiao Tong University (CN)
- Shanghai Municipal Center For Disease Control Prevention (CN)
- Corresponding author
- Wang, Jin
- Zhang, Juanbin
- Zen, Ke
- Zhang, Chen-Yu
- ORCID
- Venue
- Cell Research
- Published
- 2008-09-02
- Volume
- 18
- Issue
- 10
- Pages
- 997-1006
- ISSN
- 1001-0602 (ISSN-L)
- 1748-7838
- OpenAlex
- W2097233439
- Cited by
- 4,560
- References
- 21
- Citations by year
- 2026: 77 · 2025: 146 · 2024: 144 · 2023: 212 · 2022: 240 · 2021: 302 · 2020: 301 · 2019: 284 · 2018: 346 · 2017: 324 · 2016: 365 · 2015: 405 · 2014: 394 · 2013: 356 · 2012: 272
- FWCI
- 44.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (30471991, 973 Program, 30570731, 30225037)
- Government of Jiangsu Province
- Natural Science Foundation of Jiangsu Province (BK2006714)
- National Key Research and Development Program of China (2006CB503909)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer-related molecular mechanisms research
- Keywords
- microRNA, serum microRNA, plasma microRNA, tumor markers, non-invasive cancer diagnosis, lung cancer, colorectal cancer, diabetes mellitus, miRNA expression profiling, miRNA stability
- MeSH terms
- Animals, Carcinoma, Non-Small-Cell Lung, Cattle, Diabetes Mellitus, Type 2, Female, Horses, Humans, Lung Neoplasms, Male, Neoplasms, Biomarkers, Tumor, Colorectal Neoplasms, Biomarkers, Reverse Transcriptase Polymerase Chain Reaction, MicroRNAs, Mice, Rats
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