Cited by 96Natural Compounds in Disease TreatmentNF-κB Signaling PathwaysPhytochemicals and Antioxidant Activities

Anthocyanin prevents CD40-activated proinflammatory signaling in endothelial cells by regulating cholesterol distribution

Xia, Min, Ling, Wenhua, Zhu, Huilian, Wang, Qing, Ma, Jing, Hou, Mengjun, Tang, Zhihong, Li, Lan +1 more·2007·Arteriosclerosis Thrombosis and Vascular Biology

Abstract

OBJECTIVE: Intracellular tumor necrosis factor receptor-associated factors (TRAFs) translocation to lipid rafts is a key element in CD40-induced signaling. The purpose of this study was to investigate the influence of anthocyanin on CD40-mediated proinflammatory events in human endothelial cells and the underlying possible molecular mechanism. METHODS AND RESULTS: Treatment of endothelial cells with anthocyanin prevented from CD40-induced proinflammatory status, measured by production of IL-6, IL-8, and monocyte chemoattractant protein-1 through inhibiting CD40-induced nuclear factor-kappaB (NF-kappaB) activation. TRAF-2 played pivotal role in CD40-NF-kappaB pathway as TRAF-2 small interference RNA (siRNA) diminished CD40-induced NF-kappaB activation and inflammation. TRAF-2 overexpression increased CD40-mediated NF-kappaB activation. Moreover, TRAF-2 almost totally recruited to lipid rafts after stimulation by CD40 ligand and depletion of cholesterol diminished CD40-mediated NF-kappaB activation. Exposure to anthocyanin not only interrupted TRAF-2 recruitment to lipid rafts but also decreased cholesterol content in Triton X-100 insoluble lipid rafts. However, anthocyanin did not influence the interaction between CD40 ligand and CD40 receptor. CONCLUSIONS: Our findings suggest that anthocyanin protects from CD40-induced proinflammatory signaling by preventing TRAF-2 translocation to lipid rafts through regulation of cholesterol distribution, which thereby may represent a mechanism that would explain the anti-inflammatory response of anthocyanin.

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Crossref · 10.1161/01.atv.0000254672.04573.2d

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  • Berry anthocyanins affect lipid metabolism in cellular and animal models of dyslipidemia.

    “Berry anthocyanins have also been shown to affect lipid metabolism in cellular and animal models of dyslipidemia.”

    From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [72]

  • Anthocyanin treatment of human umbilical vein endothelial cells regulates cholesterol distribution by interfering with the recruitment of TRAF-2 in lipid rafts, thereby inhibiting CD40-induced proinflammatory signaling.

    “Anthocyanin treatment of human umbilical vein endothelial cells was further demonstrated to regulate cholesterol distribution by interfering with the recruitment of tumor necrosis factor receptor-associated factors (TRAF)-2 in lipid rafts, thereby inhibiting CD40-induced proinflammatory signaling.”

    From Berries: emerging impact on cardiovascular health (2010) · cites this paper as [72]

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Authors
Xia, M., Ling, W., Zhu, H., Wang, Q., Ma, J., Hou, M., Tang, Z., Li, L., & Ye, Q.
Affiliations
Sun Yat-sen University (CN)
Venue
Arteriosclerosis Thrombosis and Vascular Biology
Published
2007-03
Volume
27
Issue
3
Pages
519-524
ISSN
  • 1079-5642 (ISSN-L)
  • 1524-4636
OpenAlex
W2074317596
Cited by
96
References
33
Citations by year
2025: 2 · 2024: 5 · 2023: 2 · 2022: 3 · 2021: 6 · 2020: 6 · 2019: 5 · 2018: 3 · 2017: 4 · 2016: 5 · 2015: 6 · 2014: 5 · 2013: 8 · 2012: 6
FWCI
11.78
Type
journal article
Language
en
Open access
closed
Locations
  • Arteriosclerosis Thrombosis and Vascular Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
Keywords
anthocyanins, CD40 signaling, TRAF2, lipid rafts, cholesterol distribution, NF-κB activation, endothelial inflammation, inflammatory cytokines, anti-inflammatory activity, CD40 ligand, siRNA knockdown
MeSH terms
Analysis of Variance, Anthocyanins, Arteriosclerosis, Cholesterol, Humans, Inflammation, Probability, Sensitivity and Specificity, Immunoblotting, Signal Transduction, CD40 Antigens, Endothelial Cells, TNF Receptor-Associated Factor 2

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