The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Ascorbic Acid Enhances Differentiation of Embryonic Stem Cells Into Cardiac Myocytes
Abstract
Background— Embryonic stem (ES) cells are capable of self-renewal and differentiation into cellular derivatives of all 3 germ layers. In appropriate culture conditions, ES cells can differentiate into specialized cells, including cardiac myocytes, but the efficiency is typically low and the process is incompletely understood. Methods and Results— We evaluated a chemical library for its potential to induce cardiac differentiation of ES cells in the absence of embryoid body formation. Using ES cells stably transfected with cardiac-specific α-cardiac myosin heavy chain (MHC) promoter-driven enhanced green fluorescent protein (EGFP), 880 compounds approved for human use were screened for their ability to induce cardiac differentiation. Treatment with ascorbic acid, also known as vitamin C, markedly increased the number of EGFP-positive cells, which displayed spontaneous and rhythmic contractile activity and stained positively for sarcomeric myosin and α-actinin. Furthermore, ascorbic acid induced the expression of cardiac genes, including GATA4, α-MHC, and β-MHC in untransfected ES cells in a developmentally controlled manner. This effect of ascorbic acid on cardiac differentiation was not mimicked by the other antioxidants such as N-acetylcysteine, Tiron, or vitamin E. Conclusions— Ascorbic acid induces cardiac differentiation in ES cells. This study demonstrates the potential for chemically modifying the cardiac differentiation program of ES cells.
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Cites
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Ascorbic acid: much more than just an antioxidant
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Cited by
Chemically defined generation of human cardiomyocytes
Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., Lan, F., Diecke, S., Huber, B., Mordwinkin, N. M., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2999 · Cited by 1,606
Laflamme, M. A., & Murry, C. E. (2005) · Nature Biotechnology · doi:10.1038/nbt1117 · Cited by 964
Increased Cardiomyocyte Differentiation from Human Embryonic Stem Cells in Serum‐Free Cultures
Passier, R., Oostwaard, D. W., Snapper, J., Kloots, J., Hassink, R. J., Kuijk, E., Roelen, B., de la Riviere, A. B., & Mummery, C. (2005) · STEM CELLS · doi:10.1634/stemcells.2004-0184 · Cited by 324
A role for chemistry in stem cell biology
Ding, S., & Schultz, P. G. (2004) · Nature Biotechnology · doi:10.1038/nbt987 · Cited by 311
Capturing Cardiogenesis in Gastruloids
Rossi, G., Broguiere, N., Miyamoto, M., Boni, A., Guiet, R., Girgin, M., Kelly, R. G., Kwon, C., & Lutolf, M. P. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2020.10.013 · Cited by 306
Metadata
- Authors
- Takahashi, T., Lord, B., Schulze, P. C., Fryer, R. M., Sarang, S. S., Gullans, S. R., & Lee, R. T.
- Venue
- Circulation
- Published
- 2003-04-15
- Volume
- 107
- Issue
- 14
- Pages
- 1912-1916
- ISSN
- 0009-7322 (ISSN-L)
- 1524-4539
- OpenAlex
- W2119288897
- Cited by
- 477
- References
- 12
- FWCI
- 8.26
- Type
- journal article
- Language
- en
- Open access
- closed
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- The paper
- Takahashi, T., Lord, B., Schulze, P. C., Fryer, R. M., Sarang, S. S., Gullans, S. R., & Lee, R. T. (2003). Ascorbic Acid Enhances Differentiation of Embryonic Stem Cells Into Cardiac Myocytes. Circulation. https://doi.org/10.1161/01.cir.0000064899.53876.a3
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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