The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Increased Cardiomyocyte Differentiation from Human Embryonic Stem Cells in Serum‐Free Cultures
Abstract
Human embryonic stem cells (hESCs) can differentiate into cardiomyocytes, but the efficiency of this process is low. We routinely induce cardiomyocyte differentiation of the HES-2 cell line by coculture with a visceral endoderm-like cell line, END-2, in the presence of 20% fetal calf serum (FCS). In this study, we demonstrate a striking inverse relationship between cardiomyocyte differentiation and the concentration of FCS during HES-2-END-2 coculture. The number of beating areas in the cocultures was increased 24-fold in the absence of FCS compared with the presence of 20% FCS. An additional 40% increase in the number of beating areas was observed when ascorbic acid was added to serum-free cocultures. The increase in serum-free cocultures was accompanied by increased mRNA and protein expression of cardiac markers and of Isl1, a marker of cardiac progenitor cells. The number of beating areas increased up to 12 days after initiation of coculture of HES-2 with END-2 cells. However, the number of alpha-actinin-positive cardiomyocytes per beating area did not differ significantly between serum-free cocultures (503 +/- 179; mean +/- standard error of the mean) and 20% FCS cocultures (312 +/- 227). The stimulating effect of serum-free coculture on cardiomyocyte differentiation was observed not only in HES-2 but also in the HES-3 and HES-4 cell lines. To produce sufficient cardiomyocytes for cell replacement therapy in the future, upscaling cardiomyocyte formation from hESCs is essential. The present data provide a step in this direction and represent an improved in vitro model, without interfering factors in serum, for testing other factors that might promote cardiomyocyte differentiation.
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Embryonic Stem Cell Lines Derived from Human Blastocysts
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Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro
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Self-citationCharacterization and Enrichment of Cardiomyocytes Derived From Human Embryonic Stem Cells
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Ascorbic Acid Enhances Differentiation of Embryonic Stem Cells Into Cardiac Myocytes
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Hormones, Growth Factors, and Myogenic Differentiation
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Cited by
Burridge, P. W., Keller, G., Gold, J. D., & Wu, J. C. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2011.12.013 · Cited by 670
Bauwens, C. L., Peerani, R., Niebruegge, S., Woodhouse, K. A., Kumacheva, E., Husain, M., & Zandstra, P. W. (2008) · Stem Cells · doi:10.1634/stemcells.2008-0183 · Cited by 447
NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes
Elliott, D. A., Braam, S. R., Koutsis, K., Ng, E. S., Jenny, R., Lagerqvist, E. L., Biben, C., Hatzistavrou, T., Hirst, C. E., Yu, Q. C., et al. (2011) · Nature Methods · doi:10.1038/nmeth.1740 · Cited by 426
Self-citationBurridge, P. W., Anderson, D., Priddle, H., Barbadillo Muñoz, M. D., Chamberlain, S., Allegrucci, C., Young, L. E., & Denning, C. (2007) · Stem Cells · doi:10.1634/stemcells.2006-0598 · Cited by 301
Scalable Stirred-Suspension Bioreactor Culture of Human Pluripotent Stem Cells
Kehoe, D. E., Jing, D., Lock, L. T., & Tzanakakis, E. S. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2009.0454 · Cited by 264
Metadata
- Authors
- Passier, R., Oostwaard, D. W., Snapper, J., Kloots, J., Hassink, R. J., Kuijk, E., Roelen, B., de la Riviere, A. B., & Mummery, C.
- Venue
- STEM CELLS
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-06
- Volume
- 23
- Issue
- 6
- Pages
- 772-780
- ISSN
- 1066-5099 (ISSN-L)
- 1549-4918
- OpenAlex
- W1966127544
- Cited by
- 324
- References
- 20
- FWCI
- 11.52
- Type
- journal article
- Language
- en
- Open access
- closed
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- Passier, R., Oostwaard, D. W., Snapper, J., Kloots, J., Hassink, R. J., Kuijk, E., Roelen, B., de la Riviere, A. B., & Mummery, C. (2005). Increased Cardiomyocyte Differentiation from Human Embryonic Stem Cells in Serum‐Free Cultures. STEM CELLS. https://doi.org/10.1634/stemcells.2004-0184
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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