Increased Hyaluronic Acid in the Inner Renal Medulla of Obese Dogs
Abstract
Abstract Dogs placed on a high-fat diet develop obesity and hypertension associated with marked sodium retention that is due to increased tubular reabsorption. Previous studies showed that renal interstitial hydrostatic pressure is elevated in obese dogs compared with lean dogs, and histological studies revealed increases in medullary interstitial cells and expansion of the medullary but not the cortical extracellular matrix. This matrix stained intensively with Alcian Blue at pH 2.6, colloidal iron, and periodic acid–Schiff, suggesting increased glycosaminoglycans. The goal of this study was to quantitate medullary glycosaminoglycan content in obese (n=8) compared with lean (n=8) dogs. Measurement of total glycosaminoglycan content, estimated from uronic acid content, and of hyaluronate, the most abundant glycosaminoglycan in canine renal medulla, with an enzyme-linked immunosorbent assay indicated that there were no significant differences in total glycosaminoglycan or hyaluronate contents in the outer medulla of obese dogs compared with those in lean dogs. In contrast, in the inner medulla of obese dogs there was a 140% increase in hyaluronate compared with the content in lean dogs (4.3±0.5 versus 1.8±0.2 mg hyaluronate per gram wet tissue, respectively; P <.05); however, total glycosaminoglycan content was not significantly different (6.9±0.7 versus 6.2±0.5 mg uronic acid per gram wet tissue) in obese and lean dogs. These results suggest a change in the relative proportion of the glycosaminoglycan species in the inner medulla of obese dogs, with a selective increase in hyaluronate. These changes in extracellular matrix could contribute to the altered intrarenal physical forces, tubular compression, and increased tubular reabsorption observed in obese dogs.
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In obese rabbits, dogs and humans, the levels of renal medullary glycosaminoglycans, especially hyaluronan, were markedly elevated compared with lean controls.
“In obese rabbits, dogs and humans, the levels of renal medullary glycosaminoglycans, especially hyaluronan, which is an important component of the ECM and is often associated with tissue inflammation and oedema, were markedly elevated compared with lean controls53–55.”
From Obesity, kidney dysfunction and hypertension: mechanistic links (2019) · cites this paper as [54]
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- Authors
- Alonso-Galicia, M., Dwyer, T. M., Herrera, G. A., & Hall, J. E.
- Affiliations
- From the Department of Physiology and Biophysics (M.A.-G., T.M.D., J.E.H.), University of Mississippi Medical Center, Jackson, and the Department of Surgical Pathology (G.A.H.), University of Alabama at Birmingham.
- University of Mississippi Medical Center (US)
- ORCID
- Hall, John E.
- Venue
- Hypertension
- Published
- 1995-04
- Volume
- 25
- Issue
- 4
- Pages
- 888-892
- ISSN
- 0194-911X (ISSN-L)
- 1524-4563
- OpenAlex
- W1967902501
- Cited by
- 32
- References
- 24
- Citations by year
- 2025: 3 · 2024: 2 · 2023: 1 · 2022: 2 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2015: 1 · 2014: 1
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Hypertension (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl023502-13, 5r01hl039399-05)
- National Heart, Lung, and Blood Institute (HL-23502, HL‐51971, HL-39399, P01HL051971)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Health Sciences › Veterinary › Small Animals › Veterinary Medicine and Surgery
- Health Sciences › Medicine › Nephrology › Chronic Kidney Disease and Diabetes
- Keywords
- hyaluronic acid, renal medulla, obesity, glycosaminoglycans, uronic acid, high-fat diet, dogs, sodium retention, tubular reabsorption, extracellular matrix, inner medulla
- MeSH terms
- Animals, Dogs, Hyaluronic Acid, Kidney Medulla, Obesity, Reference Values, Uronic Acids
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