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Cited by 68Proteoglycans and glycosaminoglycans researchChronic Kidney Disease and DiabetesAdipokines, Inflammation, and Metabolic Diseases

Distribution of renal medullary hyaluronan in lean and obese rabbits

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Dwyer, Terry M., Banks, Shandra A., Alonso-Galicia, Magdalena, Cockrell, Kathy, Carroll, Joan F., Bigler, Stephen A., Hall, John E.·2000·Kidney International
DOI 10.1046/j.1523-1755.2000.00218.x
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Abstract

Background Obese individuals have an expanded interstitium in the renal inner medulla (IM), which stains positively with periodic acid-Schiff and Alcian blue. In obese dogs, the IM is also expanded, with hyaluronan (HA) content being 2.4 times control.Methods We determined the anatomic pattern of renal HA deposition following weight gain, using an animal model of obesity consisting of young rabbits (N = 10), representing animals entering into the study, lean rabbits (N = 19), fed a control diet, and obese rabbits (N = 19), fed a high-fat diet (15% fat, by fortifying with corn oil and lard, in a ratio of 2:1) for two to three months. Tissue was papain digested, and HA was recovered in a phosphate or a Tris buffer and detected by an indirect immunoabsorbent competition assay.Results Rabbits fed a high-fat diet for 8 to 12 weeks gained weight (37%) and became mildly hypertensive (10 mm Hg). In lean rabbits, HA was low in the renal cortex (6 +/- 30 microg/g tissue), increased steadily across the outer medulla (OM; 79 +/- 28 microg/g tissue) and was uniformly high in the IM (192 +/- 28 microg/g tissue) when recovered in a Tris buffer; these levels of tissue HA did not change during the three-month period of dietary intervention. In obese rabbits, the renal medullary interstitium was expanded and stained intensely with periodic acid Schiff and Alcian blue, and tissue HA was elevated in the IM (448 +/- 25 microg/g tissue) but not the cortex (5 +/- 25 microg/g tissue) or the OM (85 +/- 25 microg/g tissue). The significant difference was due to those IM samples taken from the renal papilla; IM samples from the body of the kidney did not significantly differ among the lean, obese, and young rabbits.Conclusion The elevated renal HA associated with weight gain is limited to the IM and occurs most consistently in the papilla, which is the region of the kidney that is most vulnerable to distention caused by elevated renal interstitial hydrostatic pressure.

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  • In obese rabbits, dogs and humans, the levels of renal medullary glycosaminoglycans, especially hyaluronan, were markedly elevated compared with lean controls.

    “In obese rabbits, dogs and humans, the levels of renal medullary glycosaminoglycans, especially hyaluronan, which is an important component of the ECM and is often associated with tissue inflammation and oedema, were markedly elevated compared with lean controls53–55.”

    From Obesity, kidney dysfunction and hypertension: mechanistic links (2019) · cites this paper as [55]

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Authors
Dwyer, T. M., Banks, S. A., Alonso-Galicia, M., Cockrell, K., Carroll, J. F., Bigler, S. A., & Hall, J. E.
Affiliations
  • University of Mississippi Medical Center (US)
  • University of North Texas Health Science Center (US)
  • Medical College of Wisconsin (US)
Corresponding author
Dwyer, Terry M.
ORCID
Hall, John E.
Venue
Kidney International
Publisher
Elsevier BV
Published
2000-08
Volume
58
Issue
2
Pages
721-729
ISSN
0085-2538 (ISSN-L)
DOI
10.1046/j.1523-1755.2000.00218.x
OpenAlex
W2085509335
Cited by
68
References
38
Citations by year
2026: 1 · 2025: 1 · 2024: 4 · 2023: 5 · 2022: 5 · 2021: 6 · 2020: 4 · 2019: 2 · 2018: 3 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 1 · 2012: 4
FWCI
1.41
Type
journal article
Language
en
Open access
Licence
Locations
  • Kidney International (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (HL‐51971, P01HL051971)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Health Sciences › Medicine › Nephrology › Chronic Kidney Disease and Diabetes
  • Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
Keywords
hyaluronic acid, renal medulla, obesity, high-fat diet, renal papilla, renal interstitial hydrostatic pressure, renal hypertrophy, renal cortex, renal outer medulla
MeSH terms
Age Factors, Animals, Blood Pressure, Female, Hyaluronic Acid, Hypertension, Renal, Kidney Cortex, Kidney Medulla, Obesity, Rabbits, Weight Gain

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