The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.
Two-Photon Microscopy of Cells and Tissue
Open accessAbstract
Two-photon excitation fluorescence imaging provides thin optical sections from deep within thick, scattering specimens by way of restricting fluorophore excitation (and thus emission) to the focal plane of the microscope. Spatial confinement of two-photon excitation gives rise to several advantages over single-photon confocal microscopy. First, penetration depth of the excitation beam is increased. Second, because out-of-focus fluorescence is never generated, no pinhole is necessary in the detection path of the microscope, resulting in increased fluorescence collection efficiency. Third, two-photon excitation markedly reduces overall photobleaching and photodamage, resulting in extended viability of biological specimens during long-term imaging. Finally, localized excitation can be used for photolysis of caged compounds in femtoliter volumes and for diffusion measurements by two-photon fluorescence photobleaching recovery. This review aims to provide an overview of the use of two-photon excitation microscopy. Selected applications of this technique will illustrate its excellent suitability to assess cellular and subcellular events in intact, strongly scattering tissue. In particular, its capability to resolve differences in calcium dynamics between individual cardiomyocytes deep within intact, buffer-perfused hearts is demonstrated. Potential applications of two-photon laser scanning microscopy as applied to integrative cardiac physiology are pointed out.
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Cites
Two-photon laser scanning fluorescence microscopy
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Nonlinear magic: multiphoton microscopy in the biosciences
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Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
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Calcium Sparks: Elementary Events Underlying Excitation-Contraction Coupling in Heart Muscle
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Two-Photon Imaging of Lymphocyte Motility and Antigen Response in Intact Lymph Node
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Thiol Reactive Probes and Chemosensors
Peng, H., Chen, W., Cheng, Y., Hakuna, L., Strongin, R., & Wang, B. (2012) · Sensors · doi:10.3390/s121115907 · Cited by 275
A global view of protein expression in human cells, tissues, and organs
Pontén, F., Gry, M., Fagerberg, L., Lundberg, E., Asplund, A., Berglund, L., Oksvold, P., Björling, E., Hober, S., Kampf, C., et al. (2009) · Molecular Systems Biology · doi:10.1038/msb.2009.93 · Cited by 222
A structural remedy toward bright dipolar fluorophores in aqueous media
Singha, S., Kim, D., Roy, B., Sambasivan, S., Moon, H., Rao, A. S., Kim, J. Y., Joo, T., Park, J. W., Rhee, Y. M., et al. (2015) · Chemical Science · doi:10.1039/c5sc01076d · Cited by 170
Two-Photon Absorption: An Open Door to the NIR-II Biological Window?
Shaw, P. A., Forsyth, E., Haseeb, F., Yang, S., Bradley, M., & Klausen, M. (2022) · Frontiers in Chemistry · doi:10.3389/fchem.2022.921354 · Cited by 75
Metadata
- Authors
- Rubart, M.
- Venue
- Circulation Research
- Published
- 2004-12-10
- Volume
- 95
- Issue
- 12
- Pages
- 1154-1166
- ISSN
- 0009-7330 (ISSN-L)
- 1524-4571
- OpenAlex
- W2114135413
- Cited by
- 339
- References
- 65
- FWCI
- 9.48
- Type
- journal article
- Language
- en
- Open access
- bronze
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
