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Cited by 929

The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.

Epigenetic Signatures of Cigarette Smoking

Open access
Joehanes, Roby, Just, Allan C., Marioni, Riccardo E., Pilling, Luke C., Reynolds, Lindsay M., Mandaviya, Pooja R., Guan, Weihua, Xu, Tao, Elks, Cathy E., Aslibekyan, Stella, Moreno-Macias, Hortensia, Smith, Jennifer A., Brody, Jennifer A., Dhingra, Radhika, Yousefi, Paul, Pankow, James S., Kunze, Sonja, Shah, Sonia H., McRae, Allan F., Lohman, Kurt, Sha, Jin, Absher, Devin M., Ferrucci, Luigi, Zhao, Wei, Demerath, Ellen W., Bressler, Jan, Grove, Megan L., Huan, Tianxiao, Liu, Chunyu, Mendelson, Michael M., Yao, Chen, Kiel, Douglas P., Peters, Annette, Wang-Sattler, Rui, Visscher, Peter M., Wray, Naomi R., Starr, John M., Ding, Jingzhong, Rodriguez, Carlos J., Wareham, Nicholas J., Irvin, Marguerite R., Zhi, Degui, Barrdahl, Myrto, Vineis, Paolo, Ambatipudi, Srikant, Uitterlinden, André G., Hofman, Albert, Schwartz, Joel, Colicino, Elena, Hou, Lifang, Vokonas, Pantel S., Hernandez, Dena G., Singleton, Andrew B., Bandinelli, Stefania, Turner, Stephen T., Ware, Erin B., Smith, Alicia K., Klengel, Torsten, Binder, Elisabeth B., Psaty, Bruce M., Taylor, Kent D., Gharib, Sina A., Swenson, Brenton R., Liang, Liming, DeMeo, Dawn L., O’Connor, George T., Herceg, Zdenko, Ressler, Kerry J., Conneely, Karen N., Sotoodehnia, Nona, Kardia, Sharon L. R., Melzer, David, Baccarelli, Andrea A., van Meurs, Joyce B. J., Romieu, Isabelle, Arnett, Donna K., Ong, Ken K., Liu, Yongmei, Waldenberger, Melanie, Deary, Ian J., Fornage, Myriam, Levy, Daniel, London, Stephanie J.·2016·Circulation: Cardiovascular Genetics
DOI 10.1161/circgenetics.116.001506
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Abstract

Background—: DNA methylation leaves a long-term signature of smoking exposure and is one potential mechanism by which tobacco exposure predisposes to adverse health outcomes, such as cancers, osteoporosis, lung, and cardiovascular disorders. Methods and Results—: To comprehensively determine the association between cigarette smoking and DNA methylation, we conducted a meta-analysis of genome-wide DNA methylation assessed using the Illumina BeadChip 450K array on 15 907 blood-derived DNA samples from participants in 16 cohorts (including 2433 current, 6518 former, and 6956 never smokers). Comparing current versus never smokers, 2623 cytosine–phosphate–guanine sites (CpGs), annotated to 1405 genes, were statistically significantly differentially methylated at Bonferroni threshold of P <1×10 −7 (18 760 CpGs at false discovery rate <0.05). Genes annotated to these CpGs were enriched for associations with several smoking-related traits in genome-wide studies including pulmonary function, cancers, inflammatory diseases, and heart disease. Comparing former versus never smokers, 185 of the CpGs that differed between current and never smokers were significant P <1×10 −7 (2623 CpGs at false discovery rate <0.05), indicating a pattern of persistent altered methylation, with attenuation, after smoking cessation. Transcriptomic integration identified effects on gene expression at many differentially methylated CpGs. Conclusions—: Cigarette smoking has a broad impact on genome-wide methylation that, at many loci, persists many years after smoking cessation. Many of the differentially methylated genes were novel genes with respect to biological effects of smoking and might represent therapeutic targets for prevention or treatment of tobacco-related diseases. Methylation at these sites could also serve as sensitive and stable biomarkers of lifetime exposure to tobacco smoke.

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Authors
Joehanes, R., Just, A. C., Marioni, R. E., Pilling, L. C., Reynolds, L. M., Mandaviya, P. R., Guan, W., Xu, T., Elks, C. E., Aslibekyan, S., Moreno-Macias, H., Smith, J. A., Brody, J. A., Dhingra, R., Yousefi, P., Pankow, J. S., Kunze, S., Shah, S. H., McRae, A. F., Lohman, K., Sha, J., Absher, D. M., Ferrucci, L., Zhao, W., Demerath, E. W., Bressler, J., Grove, M. L., Huan, T., Liu, C., Mendelson, M. M., Yao, C., Kiel, D. P., Peters, A., Wang-Sattler, R., Visscher, P. M., Wray, N. R., Starr, J. M., Ding, J., Rodriguez, C. J., Wareham, N. J., Irvin, M. R., Zhi, D., Barrdahl, M., Vineis, P., Ambatipudi, S., Uitterlinden, A. G., Hofman, A., Schwartz, J., Colicino, E., Hou, L., Vokonas, P. S., Hernandez, D. G., Singleton, A. B., Bandinelli, S., Turner, S. T., Ware, E. B., Smith, A. K., Klengel, T., Binder, E. B., Psaty, B. M., Taylor, K. D., Gharib, S. A., Swenson, B. R., Liang, L., DeMeo, D. L., O’Connor, G. T., Herceg, Z., Ressler, K. J., Conneely, K. N., Sotoodehnia, N., Kardia, S. L. R., Melzer, D., Baccarelli, A. A., van Meurs, J. B. J., Romieu, I., Arnett, D. K., Ong, K. K., Liu, Y., Waldenberger, M., Deary, I. J., Fornage, M., Levy, D., & London, S. J.
Venue
Circulation: Cardiovascular Genetics
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2016-10
Volume
9
Issue
5
Pages
436-447
ISSN
  • 1942-325X (ISSN-L)
  • 1942-3268
DOI
10.1161/circgenetics.116.001506
OpenAlex
W2523285199
Cited by
929
References
65
FWCI
36.26
Type
journal article
Language
en
Open access
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CoChat. (2026). Citation check: Epigenetic Signatures of Cigarette Smoking. https://app.cochat.ai/papers/10.1161/circgenetics.116.001506
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Joehanes, R., Just, A. C., Marioni, R. E., Pilling, L. C., Reynolds, L. M., Mandaviya, P. R., Guan, W., Xu, T., Elks, C. E., Aslibekyan, S., Moreno-Macias, H., Smith, J. A., Brody, J. A., Dhingra, R., Yousefi, P., Pankow, J. S., Kunze, S., Shah, S. H., McRae, A. F., . . . London, S. J. (2016). Epigenetic Signatures of Cigarette Smoking. Circulation: Cardiovascular Genetics. https://doi.org/10.1161/circgenetics.116.001506

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

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