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Induction of Endothelial NO Synthase by Hydrogen Peroxide via a Ca 2+ /Calmodulin-Dependent Protein Kinase II/Janus Kinase 2–Dependent Pathway
Open accessAbstract
We have recently demonstrated that hydrogen peroxide (H 2 O 2 ) is an extremely potent stimulus of endothelial NO synthase (eNOS) gene expression. The present study was designed to identify the signaling mechanisms mediating this response. Induction of eNOS expression by H 2 O 2 was found to be Ca 2+ dependent, inasmuch as it was blocked by BAPTA-AM. Further studies have indicated that Ca 2+ /calmodulin-dependent protein kinase II (CaM kinase II) plays a critical role in mediating this response. Immunocytochemical staining with an anti-CaM kinase II antibody confirmed the expression of CaM kinase II in cultured bovine aortic endothelial cells. H 2 O 2 induced autophosphorylation of CaM kinase II and increased the activity of the enzyme, as assessed by an in-gel kinase assay. A specific inhibitor for CaM kinase II, KN93, and a calmodulin antagonist, W-7, attenuated eNOS induction by H 2 O 2 . Further studies have indicated that janus kinase 2 is important in mediating increased eNOS expression in response to H 2 O 2 and likely is downstream from CaM kinase II. In conclusion, these data provide the first evidence that CaM kinase II plays a critical role in endothelial redox signaling. Regulation of eNOS via this pathway may represent an important vascular adaptation to oxidant stress.
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“The signaling mechanisms of hydrogen peroxide in ECs (941) lead to the increased activity of eNOS and other enzymes (121, 141, 193, 251, 815, 940).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [141]
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Metadata
- Authors
- Cai, H., Davis, M. E., Drummond, G. R., & Harrison, D. G.
- Affiliations
- Emory University (US)
- Venue
- Arteriosclerosis, Thrombosis, and Vascular Biology
- Published
- 2001-10
- Volume
- 21
- Issue
- 10
- Pages
- 1571-1576
- ISSN
- 1079-5642 (ISSN-L)
- 1524-4636
- OpenAlex
- W2040801242
- Cited by
- 160
- References
- 34
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 8 · 2023: 4 · 2022: 3 · 2021: 5 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 8 · 2015: 6 · 2014: 5 · 2013: 8 · 2012: 14
- FWCI
- 6.88
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Arteriosclerosis Thrombosis and Vascular Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Veterans Affairs
- National Institutes of Health (5r01hl059248-04)
- National Heart, Lung, and Blood Institute (HL 58000, HL 59248, HL 39000 6, P01HL058000)
- Topics
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Keywords
- endothelial nitric oxide synthase, hydrogen peroxide, calcium/calmodulin-dependent protein kinase II, JAK2, oxidative stress, eNOS gene expression, autophosphorylation, calmodulin, KN-93, W-7, bovine aortic endothelial cells
- MeSH terms
- Benzenesulfonamides, Animals, Benzylamines, Calcium, Cattle, Cells, Cultured, Endothelium, Vascular, Enzyme Inhibitors, Hydrogen Peroxide, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, RNA, Messenger, Sulfonamides, Signal Transduction, Transcriptional Activation, Calcium-Calmodulin-Dependent Protein Kinases, Nitric Oxide Synthase, Nitric Oxide Synthase Type III, Janus Kinase 2, Calcium-Calmodulin-Dependent Protein Kinase Type 2
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