The DOI matches its Crossref record; 30 of the 31 references it cites were found in a registry or an index.
Hydroxysteroid (17-β) Dehydrogenase 1 Is Dysregulated by Mir-210 and Mir-518c That Are Aberrantly Expressed in Preeclamptic Placentas: A Novel Marker for Predicting Preeclampsia
Abstract
In this study, to search for novel preeclampsia (PE) biomarkers, we focused on microRNA expression and function in the human placenta complicated with PE. By comprehensive analyses of microRNA expression, we identified 22 microRNAs significantly upregulated in preeclamptic placentas, 5 of which were predicted in silico to commonly target the mRNA encoding hydroxysteroid (17-β) dehydrogenase 1 (HSD17B1), a steroidogenetic enzyme expressed predominantly in the placenta. In vivo HSD17B1 expression, at both the mRNA and protein levels, was significantly decreased in preeclamptic placentas. Of these microRNAs, miR-210 and miR-518c were experimentally validated to target HSD17B1 by luciferase assay, real-time PCR, and ELISA. Furthermore, we found that plasma HSD17B1 protein levels in preeclamptic pregnant women reflected the decrease of its placental expression. Moreover, a prospective cohort study of plasma HSD17B1 revealed a significant reduction of plasma HSD17B1 levels in pregnant women at 20 to 23 and 27 to 30 weeks of gestation before PE onset compared with those with normal pregnancies. The sensitivities/specificities for predicting PE at 20 to 23 and 27 to 30 weeks of gestation were 0.75/0.67 (cutoff value=21.9 ng/mL) and 0.88/0.51 (cutoff value=30.5 ng/mL), and the odds ratios were 6.09 (95% CI: 2.35–15.77) and 7.83 (95% CI: 1.70–36.14), respectively. We conclude that HSD17B1 is dysregulated by miR-210 and miR-518c that are aberrantly expressed in preeclamptic placenta and that reducing plasma level of HSD17B1 precedes the onset of PE and is a potential prognostic factor for PE.
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In the citing paper
“It is increased in normal placentas from pregnancies at high altitude (121), and in placentas from pregnancies complicated by pre-eclampsia (272, 351, 401, 611).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [272]
In the citing paper
“Besides mitochondria, other targets identified for miR-210 include the steroidogenic enzyme hydroxysteroid (17-ß) dehydrogenase 1 (272), ephrin-A3 and homeobox-A9 which are involved in trophoblast cell migration and vascular remodeling (611), and thrombospondin type 1 domain containing 7A in the placental vasculature (351).”
From Placental Origins of Chronic Disease (2016) · cites this paper as [272]
Citations
Cites
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A Mammalian microRNA Expression Atlas Based on Small RNA Library Sequencing
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Steegers, E. A., von Dadelszen, P., Duvekot, J. J., & Pijnenborg, R. (2010) · The Lancet · doi:10.1016/s0140-6736(10)60279-6 · Cited by 2,850
Identification of hundreds of conserved and nonconserved human microRNAs
Bentwich, I., Avniel, A., Karov, Y., Aharonov, R., Gilad, S., Barad, O., Barzilai, A., Einat, P., Einav, U., Meiri, E., et al. (2005) · Nature Genetics · doi:10.1038/ng1590 · Cited by 1,841
Soluble Endoglin and Other Circulating Antiangiogenic Factors in Preeclampsia
Levine, R. J., Lam, C., Qian, C., Yu, K. F., Maynard, S. E., Sachs, B. P., Sibai, B. M., Epstein, F. H., Romero, R. J., Thadhani, R., et al. (2006) · New England Journal of Medicine · doi:10.1056/nejmoa055352 · Cited by 1,823
Placental Origins of Preeclampsia
Huppertz, B. (2008) · Hypertension · doi:10.1161/hypertensionaha.107.107607 · Cited by 899
Peltier, H. J., & Latham, G. J. (2008) · RNA · doi:10.1261/rna.939908 · Cited by 820
Preeclampsia: The Role of Angiogenic Factors in Its Pathogenesis
Wang, A., Rana, S., & Karumanchi, S. A. (2009) · Physiology · doi:10.1152/physiol.00043.2008 · Cited by 561
Luo, S.-S., Ishibashi, O., Ishikawa, G., Ishikawa, T., Katayama, A., Mishima, T., Takizawa, T., Shigihara, T., Goto, T., Izumi, A., et al. (2009) · Biology of Reproduction · doi:10.1095/biolreprod.108.075481 · Cited by 489
Self-citationPineles, B. L., Romero, R., Montenegro, D., Tarca, A. L., Han, Y. M., Kim, Y. M., Draghici, S., Espinoza, J., Kusanovic, J. P., Mittal, P., et al. (2007) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2007.01.008 · Cited by 450
Cited by
Sorted by citations
Placental Origins of Chronic Disease
Burton, G. J., Fowden, A. L., & Thornburg, K. L. (2016) · Physiological Reviews · doi:10.1152/physrev.00029.2015 · Cited by 717
Ji, L., Brkić, J., Liu, M., Fu, G., Peng, C., & Wang, Y. (2012) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2012.12.008 · Cited by 395
MicroRNAs in Human Placental Development and Pregnancy Complications
Fu, G., Brkić, J., Hayder, H., & Peng, C. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms14035519 · Cited by 293
Chatuphonprasert, W., Jarukamjorn, K., & Ellinger, I. (2018) · Frontiers in Pharmacology · doi:10.3389/fphar.2018.01027 · Cited by 286
Acestor, N., Goett, J., Lee, A. M., Herrick, T. M., Engelbrecht, S. M., Harner-Jay, C. M., Howell, B. J., & Weigl, B. H. (2015) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2015-0069 · Cited by 270
Metadata
- Authors
- Ishibashi, O., Ohkuchi, A., Ali, M. M., Kurashina, R., Luo, S.-S., Ishikawa, T., Takizawa, T., Hirashima, C., Takahashi, K., Migita, M., Ishikawa, G., Yoneyama, K., Asakura, H., Izumi, A., Matsubara, S., Takeshita, T., & Takizawa, T.
- Affiliations
- Jichi Medical University (JP)
- ORCID
- Venue
- Hypertension
- Published
- 2012-02
- Volume
- 59
- Issue
- 2
- Pages
- 265-273
- ISSN
- 0194-911X (ISSN-L)
- 1524-4563
- OpenAlex
- W1987627565
- Cited by
- 173
- References
- 31
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 7 · 2023: 4 · 2022: 11 · 2021: 15 · 2020: 11 · 2019: 11 · 2018: 17 · 2017: 13 · 2016: 16 · 2015: 19 · 2014: 14 · 2013: 18 · 2012: 5
- FWCI
- 15.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Hypertension (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Jichi Medical University
- China Medical University
- Japan Society for the Promotion of Science (22791554, 20390437, 23390386, 23592419)
- RIKEN
- Topics
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Clinical Neurology › Neurological Complications and Syndromes
- Keywords
- preeclampsia, HSD17B1, miR-210, microRNA, placenta, preeclampsia biomarkers, steroidogenesis, prognostic factors, microRNA target regulation
- MeSH terms
- Adult, Hypoxia, Cells, Cultured, Estradiol Dehydrogenases, Female, Humans, Placenta, Pre-Eclampsia, Predictive Value of Tests, Pregnancy, Pregnancy Complications, Cardiovascular, Pregnancy Trimester, Second, Pregnancy Trimester, Third, Prognosis, Prospective Studies, ROC Curve, Trophoblasts, Cohort Studies, Biomarkers, MicroRNAs
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