Calcineurin Orchestrates Lateral Transfer of Aspergillus fumigatus during Macrophage Cell Death
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Rationale: Pulmonary aspergillosis is a lethal mold infection in the immunocompromised host. Understanding initial control of infection and how this is altered in the immunocompromised host are key goals for comprehension of the pathogenesis of pulmonary aspergillosis. Objectives: To characterize the outcome of human macrophage infection with Aspergillus fumigatus and how this is altered in transplant recipients on calcineurin inhibitor immunosuppressants. Methods: We defined the outcome of human macrophage infection with A. fumigatus, as well as the impact of calcineurin inhibitors, through a combination of single-cell fluorescence imaging, transcriptomics, proteomics, and in vivo studies. Measurements and Main Results: Macrophage phagocytosis of A. fumigatus enabled control of 90% of fungal germination. However, fungal germination in the late phagosome led to macrophage necrosis. During programmed necroptosis, we observed frequent cell–cell transfer of A. fumigatus between macrophages, which assists subsequent control of germination in recipient macrophages. Lateral transfer occurred through actin-dependent exocytosis of the late endosome in a vasodilator-stimulated phosphoprotein envelope. Its relevance to the control of fungal germination was also shown by direct visualization in our zebrafish aspergillosis model in vivo. The calcineurin inhibitor FK506 (tacrolimus) reduced cell death and lateral transfer in vitro by 50%. This resulted in uncontrolled fungal germination in macrophages and also resulted in hyphal escape. Conclusions: These observations identify programmed, necrosis-dependent lateral transfer of A. fumigatus between macrophages as an important host strategy for controlling fungal germination. This process is critically dependent on calcineurin. Our studies provide fundamental insights into the pathogenesis of pulmonary aspergillosis in the immunocompromised host.
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Ca2+-calcineurin signaling orchestrates a novel process of lateral transfer of germinating conidia between macrophages via an actin-dependent process.
“Ca2+-calcineurin signaling also orchestrates a novel process of lateral transfer of germinating conidia between macrophages via an actin-dependent process (201).”
From Aspergillus fumigatus and Aspergillosis in 2019 (2019) · cites this paper as [201]
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Kim, D., Pertea, G., Trapnell, C., Pimentel, H., Kelley, R., & Salzberg, S. L. (2013) · Genome Biology · doi:10.1186/gb-2013-14-4-r36 · Cited by 12,668 · see citing works
Trapnell, C., Roberts, A., Goff, L. A., Pertea, G. M., Kim, D., Kelley, D. R., Pimentel, H., Salzberg, S. L., Rinn, J. L., & Pachter, L. S. (2012) · Nature Protocols · doi:10.1038/nprot.2012.016 · Cited by 11,930 · see citing works
DAVID: Database for Annotation, Visualization, and Integrated Discovery
Dennis, G., Sherman, B. T., Hosack, D. A., Yang, J., Gao, W., Lane, H. C., & Lempicki, R. A. (2003) · Genome Biology · doi:10.1186/gb-2003-4-5-p3 · Cited by 9,327 · see citing works
An automated method for finding molecular complexes in large protein interaction networks
Bader, G. D., & Hogue, C. W. V. (2003) · BMC Bioinformatics · doi:10.1186/1471-2105-4-2 · Cited by 6,112 · see citing works
Maere, S., Heymans, K., & Kuiper, M. (2005) · Bioinform. · doi:10.1093/bioinformatics/bti551 · Cited by 4,251 · see citing works
STRING 8--a global view on proteins and their functional interactions in 630 organisms
Jensen, L. J., Kuhn, M., Stark, M., Chaffron, S., Creevey, C. J., Muller, J., Doerks, T., Julien, P., Roth, A. C., Simonovic, M., et al. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn760 · Cited by 2,475 · see citing works
<i>Aspergillus fumigatus</i> and Aspergillosis
Latgé, J.-P. (1999) · Clinical Microbiology Reviews · doi:10.1128/cmr.12.2.310 · Cited by 2,307 · see citing works
Pappas, P. G., Alexander, B. D., Andes, D. R., Hadley, S., Kauffman, C. A., Freifeld, A., Anaissie, E. J., Brumble, L. M., Herwaldt, L., Ito, J., et al. (2010) · Clinical Infectious Diseases · doi:10.1086/651262 · Cited by 1,570 · see citing works
Dephosphorylation by calcineurin regulates translocation of Drp1 to mitochondria
Cereghetti, G. M., Stangherlin, A., de Brito, O. M., Chang, C. R., Blackstone, C., Bernardi, P., & Scorrano, L. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0808249105 · Cited by 1,129 · see citing works
Linkermann, A., & Green, D. R. (2014) · New England Journal of Medicine · doi:10.1056/nejmra1310050 · Cited by 1,103 · see citing works
The clinical spectrum of pulmonary aspergillosis
Kosmidis, C., & Denning, D. W. (2015) · Thorax · doi:10.1136/thoraxjnl-2014-206291 · Cited by 850 · see citing works
Ena/VASP Proteins: Regulators of the Actin Cytoskeleton and Cell Migration
Krause, M., Dent, E. W., Bear, J. E., Loureiro, J., & Gertler, F. B. (2003) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.19.050103.103356 · Cited by 682 · see citing works
Mitophagy-dependent necroptosis contributes to the pathogenesis of COPD
Mizumura, K., Cloonan, S. M., Nakahira, K., Bhashyam, A. R., Cervo, M., Kitada, T., Glass, K., Owen, C. A., Mahmood, A., Washko, G. R., et al. (2014) · Journal of Clinical Investigation · doi:10.1172/jci74985 · Cited by 611 · see citing works
The diverse role of RIP kinases in necroptosis and inflammation
Silke, J., Rickard, J. A., & Gerlic, M. (2015) · Nature Immunology · doi:10.1038/ni.3206 · Cited by 522 · see citing works
Denning, D. W., Pleuvry, A., & Cole, D. C. (2013) · Medical Mycology · doi:10.3109/13693786.2012.738312 · Cited by 495 · see citing works
Taylor, A. L., Watson, C. J. E., & Bradley, J. A. (2005) · Critical Reviews in Oncology/Hematology · doi:10.1016/j.critrevonc.2005.03.012 · Cited by 446 · see citing works
Philippe, B., Ibrahim-Granet, O., Prévost, M. C., Gougerot-Pocidalo, M. A., Sanchez Perez, M., Van der Meeren, A., & Latgé, J. P. (2003) · Infection and Immunity · doi:10.1128/iai.71.6.3034-3042.2003 · Cited by 415 · see citing works
Requisite Role for the Dectin-1 β-Glucan Receptor in Pulmonary Defense against Aspergillus fumigatus
Werner, J. L., Metz, A. E., Horn, D., Schoeb, T. R., Hewitt, M. M., Schwiebert, L. M., Faro‐Trindade, I., Brown, G. D., & Steele, C. H. (2009) · The Journal of Immunology · doi:10.4049/jimmunol.0804250 · Cited by 412 · see citing works
Taccone, F. S., den Abeele, A. V., Bulpa, P., Misset, B., Meersseman, W., Cardoso, T., Paiva, J.-A., Blasco-Navalpotro, M. Á., Laere, E. D., Dimοpoulos, G., et al. (2015) · Critical Care · doi:10.1186/s13054-014-0722-7 · Cited by 405 · see citing works
Goodridge, H. S., Simmons, R. M., & Underhill, D. M. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.178.5.3107 · Cited by 359 · see citing works
VASP is a processive actin polymerase that requires monomeric actin for barbed end association
Hansen, S. D., & Mullins, R. D. (2010) · The Journal of Cell Biology · doi:10.1083/jcb.201003014 · Cited by 275 · see citing works
Coppolino, M. G., Krause, M., Hagendorff, P., Monner, D. A., Trimble, W. S., Grinstein, S., Wehland, J., & Sechi, A. S. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.23.4307 · Cited by 200 · see citing works
Mostowy, S., Boucontet, L., Mazón, M. J., Sirianni, A., Boudinot, P., Hollinshead, M. S., Cossart, P., Herbomel, P., Levraud, J., & Colucci‐Guyon, E. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003588 · Cited by 189 · see citing works
Self-citationWu, Y., Tan, H.-L., Huang, Q., Xj, S., Zhu, X., & Shen, H.-M. (2010) · Cell Death and Differentiation · doi:10.1038/cdd.2010.72 · Cited by 176 · see citing works
Maturu, V. N., & Agarwal, R. (2015) · Clinical & Experimental Allergy · doi:10.1111/cea.12595 · Cited by 172 · see citing works
Herbst, S., Shah, A., Mazón, M. J., Marzola, V., Jensen, B., Reed, A. K., Birrell, M. A., Saijo, S., Mostowy, S., Shaunak, S., et al. (2015) · EMBO Molecular Medicine · doi:10.15252/emmm.201404556 · Cited by 172 · see citing works
Self-citationInflammatory Monocytes Orchestrate Innate Antifungal Immunity in the Lung
Espinosa, V., Jhingran, A., Dutta, O., Kasahara, S., Donnelly, R., Du, P., Rosenfeld, J., Leiner, I., Chen, C.-C., Ron, Y., et al. (2014) · PLoS Pathogens · doi:10.1371/journal.ppat.1003940 · Cited by 169 · see citing works
Caspase blockade induces RIP3-mediated programmed necrosis in Toll-like receptor-activated microglia
Kim, S. J., & Li, J. (2013) · Cell Death and Disease · doi:10.1038/cddis.2013.238 · Cited by 146 · see citing works
Ader, F., Nseir, S., Berre, R. L., Leroy, S., Tillie‐Leblond, I., Marquette, C., & Durocher, A. (2005) · Clinical Microbiology and Infection · doi:10.1111/j.1469-0691.2005.01143.x · Cited by 145 · see citing works
Johnston, S. A., & May, R. C. (2010) · PLoS Pathogens · doi:10.1371/journal.ppat.1001041 · Cited by 141 · see citing works
Bhatia, S., Fei, M., Yarlagadda, M., Qi, Z., Akira, S., Saijo, S., Iwakura, Y., van Rooijen, N., Gibson, G. A., Croix, C. M. S., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0015943 · Cited by 119 · see citing works
Virulent Burkholderia Species Mimic Host Actin Polymerases to Drive Actin-Based Motility
Benanti, E. L., Nguyen, C. M., & Welch, M. D. (2015) · Cell · doi:10.1016/j.cell.2015.02.044 · Cited by 113 · see citing works
Allergic Bronchopulmonary Aspergillosis and Related Allergic Syndromes
Hogan, C., & Denning, D. W. (2011) · Seminars in Respiratory and Critical Care Medicine · doi:10.1055/s-0031-1295716 · Cited by 89 · see citing works
Nuclear Factor of Activated T Cells c Is a Target of p38 Mitogen-Activated Protein Kinase in T Cells
Wu, C., Hsu, S., Shih, H., & Lai, M. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.18.6442-6454.2003 · Cited by 86 · see citing works
Calcium-dependent interaction of calcineurin with bcl-2 in neuronal tissue
Erin, N., Bronson, S. K., & Billingsley, M. L. (2003) · Neuroscience · doi:10.1016/s0306-4522(02)00933-8 · Cited by 70 · see citing works
Catching Fire: Candida albicans, Macrophages, and Pyroptosis
Krysan, D. J., Sutterwala, F. S., & Wellington, M. A. (2014) · PLoS Pathogens · doi:10.1371/journal.ppat.1004139 · Cited by 68 · see citing works
The Phosphorylation-Dependent Regulation of Mitochondrial Proteins in Stress Responses
Kanamaru, Y., Sekine, S., Ichijo, H., & Takeda, K. (2012) · Journal of Signal Transduction · doi:10.1155/2012/931215 · Cited by 56 · see citing works
Tourneur, E., Mkaddem, S. B., Chassin, C., Bens, M., Goujon, J., Charles, N., Pellefigues, C., Aloulou, M., Hertig, A., Monteiro, R. C., et al. (2013) · PLoS Pathogens · doi:10.1371/journal.ppat.1003152 · Cited by 49 · see citing works
Regulation of Cofilin Activity by CaMKII and Calcineurin
Zhao, J.-W., Gao, Z., Zhang, H., Ji, Q., Wang, H., Yang, Y., Xing, F., Meng, L.-J., & Wang, Y. (2012) · The American Journal of the Medical Sciences · doi:10.1097/maj.0b013e318244745b · Cited by 45 · see citing works
The biology of pulmonary aspergillus infections
Warris, A. (2014) · Journal of Infection · doi:10.1016/j.jinf.2014.07.011 · Cited by 44 · see citing works
Chen, G.-H., Teitz‐Tennenbaum, S., Neal, L. M., Murdock, B. J., Malachowski, A. N., Dils, A. J., Olszewski, M. A., & Osterholzer, J. J. (2016) · The Journal of Immunology · doi:10.4049/jimmunol.1501512 · Cited by 43 · see citing works
A new and clinically relevant murine model of solid-organ transplant aspergillosis
Herbst, S., Shah, A., Carby, M. R., Chusney, G. D., Naresh, K. N., Dorling, A., Bignell, E., Shaunak, S., & Armstrong-James, D. P. H. (2012) · Disease Models & Mechanisms · doi:10.1242/dmm.010330 · Cited by 40 · see citing works
Self-citationHuang, C.-C., Wang, J.-M., Kikkawa, U., Mukai, H., Shen, M.-R., Morita, I., Chen, B.-K., & Chang, W.-C. (2007) · Oncogene · doi:10.1038/sj.onc.1210888 · Cited by 38 · see citing works
Fueling the Flames: Mammalian Programmed Necrosis in Inflammatory DiseasesPreprint
Chan, F. K.-M. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a008805 · Cited by 37 · see citing works
Specific calcineurin targeting in macrophages confers resistance to inflammation via MKP‐1 and p38
Escolano, A., Martı́nez-Martı́nez, S., Alfranca, A., Urso, K., Izquierdo, H. M., Delgado, M., Martı́n, F., Sabio, G., Sancho, D., Arco, P. G., et al. (2014) · The EMBO Journal · doi:10.1002/embj.201386369 · Cited by 31 · see citing works
Szumowski, S. C., Estes, K. A., Popovich, J. J., Botts, M. R., Sek, G., & Troemel, E. R. (2015) · Cellular Microbiology · doi:10.1111/cmi.12481 · Cited by 29 · see citing works
Renal Allograft Recipients Fail to Increase Interferon-γ During Invasive Fungal Diseases
Armstrong-James, D. P. H., Teo, I. A., Herbst, S., Petrou, M., Shiu, K. Y., McLean, A., Taube, D. H., Dorling, A., & Shaunak, S. (2012) · American Journal of Transplantation · doi:10.1111/j.1600-6143.2012.04254.x · Cited by 17 · see citing works
Self-citationMittal, A., Gahlaut, A., Sharma, G. L., & Dabur, R. (2013) · Europe PMC (PubMed Central) · Cited by 4 · see citing works
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Latgé, J.-P., & Chamilos, G. (2019) · Clinical Microbiology Reviews · doi:10.1128/cmr.00140-18 · Cited by 1,400 · see citing works
Aspergillus fumigatus morphology and dynamic host interactions
van de Veerdonk, F. L., Gresnigt, M. S., Romani, L., Netea, M. G., & Latgé, J.-P. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2017.90 · Cited by 490 · see citing works
Roberts, M. B., & Fishman, J. A. (2020) · Clinical Infectious Diseases · doi:10.1093/cid/ciaa1189 · Cited by 283 · see citing works
Latgé, J.-P., Beauvais, A., & Chamilos, G. (2017) · Annual Review of Microbiology · doi:10.1146/annurev-micro-030117-020406 · Cited by 207 · see citing works
Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species
Andrianaki, A. M., Kyrmizi, I., Thanopoulou, K., Baldin, C., Drakos, E., Soliman, S. S. M., Shetty, A. C., McCracken, C. L., Akoumianaki, T., Stylianou, K., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-05820-2 · Cited by 133 · see citing works
Feys, S., Gonçalves, S. M., Khan, M., Choi, S., Boeckx, B., Chatelain, D., Cunha, C., Debaveye, Y. A., Hermans, G., Hertoghs, M., et al. (2022) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(22)00259-4 · Cited by 125 · see citing works
Bercusson, A., Colley, T., Shah, A., Warris, A., & Armstrong-James, D. P. H. (2018) · Blood · doi:10.1182/blood-2017-12-823393 · Cited by 116 · see citing works
Self-citationMacrophages inhibit Aspergillus fumigatus germination and neutrophil-mediated fungal killing
Rosowski, E. E., Raffa, N., Knox, B. P., Golenberg, N., Keller, N. P., & Anna Huttenlocher, A. (2018) · PLoS Pathogens · doi:10.1371/journal.ppat.1007229 · Cited by 113 · see citing works
Pathogenicity and virulence of Aspergillus fumigatus
Earle, K., Valero, C., Conn, D. P., Vere, G., Cook, P. C., Bromley, M., Bowyer, P., & Gago, S. (2023) · Virulence · doi:10.1080/21505594.2023.2172264 · Cited by 117 · see citing works
Phagosomal removal of fungal melanin reprograms macrophage metabolism to promote antifungal immunity
Gonçalves, S. M., Duarte‐Oliveira, C., Campos, C. F., Aimanianda, V., ter Horst, R., Leite, L., Mercier, T., Pereira, P. N. G., Fernández-García, M., Antunes, D., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-16120-z · Cited by 107 · see citing works
Types of necroinflammation, the effect of cell death modalities on sterile inflammation
Mázló, A., Jenei, V., Sára, B., Molnár, T., Bácsi, A., & Koncz, G. (2022) · Cell Death and Disease · doi:10.1038/s41419-022-04883-w · Cited by 68 · see citing works
Bendíčková, K., Tidu, F., & Frič, J. (2017) · EMBO Molecular Medicine · doi:10.15252/emmm.201707698 · Cited by 65 · see citing works
Interleukin 1α Is Critical for Resistance against Highly Virulent Aspergillus fumigatus Isolates
Caffrey-Carr, A. K., Kowalski, C. H., Beattie, S. R., Blaseg, N. A., Upshaw, C. R., Thammahong, A., Lust, H. E., Tang, Y., Hohl, T. M., Cramer, R. A., et al. (2017) · Infection and Immunity · doi:10.1128/iai.00661-17 · Cited by 62 · see citing works
Involvement of the Actin Machinery in Programmed Cell Death
Ren, W., Zhao, W., Cao, L., & Huang, J. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.634849 · Cited by 64 · see citing works
Ellett, F. E., Pazhakh, V., Pase, L. B., Benard, E. L., Weerasinghe, H., Azabdaftari, D., Alasmari, S., Andrianopoulos, A., & Lieschke, G. J. (2018) · PLoS Pathogens · doi:10.1371/journal.ppat.1007063 · Cited by 64 · see citing works
The Zebrafish as a Model Host for Invasive Fungal Infections
Rosowski, E. E., Knox, B. P., Archambault, L. S., Anna Huttenlocher, A., Keller, N. P., Wheeler, R. T., & Davis, J. M. (2018) · Journal of Fungi · doi:10.3390/jof4040136 · Cited by 61 · see citing works
Aspergillus fumigatus biology, immunopathogenicity and drug resistance
van de Veerdonk, F. L., Carvalho, A., Wauters, J., Chamilos, G., & Verweij, P. E. (2025) · Nature Reviews Microbiology · doi:10.1038/s41579-025-01180-z · Cited by 58 · see citing works
Novohradská, S., Ferling, I., & Hillmann, F. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00497 · Cited by 52 · see citing works
Programmed Cell Death: Central Player in Fungal Infections
Camilli, G., Blagojevic, M., Naglik, J. R., & Richardson, J. P. (2020) · Trends in Cell Biology · doi:10.1016/j.tcb.2020.11.005 · Cited by 53 · see citing works
Jia, L., Rafiq, M., Radosa, L., Hortschansky, P., Cunha, C., Cseresnyés, Z., Krüger, T., Schmidt, F., Heinekamp, T., Straßburger, M., et al. (2022) · Cell Host & Microbe · doi:10.1016/j.chom.2023.02.002 · Cited by 42 · see citing works
Linnerz, T., & Hall, C. J. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.01094 · Cited by 41 · see citing works
A Fun-Guide to Innate Immune Responses to Fungal Infections
Burgess, T. B., Condliffe, A. M., & Elks, P. M. (2022) · Journal of Fungi · doi:10.3390/jof8080805 · Cited by 43 · see citing works
Seldeslachts, L., Staels, F., Gkountzinopoulou, M., Jacobs, C., Tielemans, B., Vanhoffelen, E., Reséndiz-Sharpe, A., Herdt, L. D., Haughton, J., Prezzemolo, T., et al. (2024) · EBioMedicine · doi:10.1016/j.ebiom.2024.105347 · Cited by 37 · see citing works
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Yoshida, N., Frickel, E., & Mostowy, S. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.01703 · Cited by 34 · see citing works
Self-citationVomocytosis: What we know so far
Seoane, P. I., & May, R. C. (2019) · Cellular Microbiology · doi:10.1111/cmi.13145 · Cited by 36 · see citing works
Eades, C. P., & Armstrong-James, D. P. H. (2018) · Medical Mycology · doi:10.1093/mmy/myy136 · Cited by 35 · see citing works
Self-citation“Under Pressure” – How fungi evade, exploit, and modulate cells of the innate immune system
Lange, T., Kasper, L., Gresnigt, M., Brunke, S., & Hube, B. (2023) · Seminars in Immunology · doi:10.1016/j.smim.2023.101738 · Cited by 29 · see citing works
Pazhakh, V., Ellett, F. E., Croker, B. A., O’Donnell, J. A., Pase, L. B., Schulze, K. E., Greulich, R. S., Gupta, A., Reyes‐Aldasoro, C. C., Andrianopoulos, A., et al. (2019) · PLoS Biology · doi:10.1371/journal.pbio.3000113 · Cited by 30 · see citing works
Fungal-Induced Programmed Cell Death
Williams, T. J., Gonzales-Huerta, L. E., & Armstrong-James, D. P. H. (2021) · Journal of Fungi · doi:10.3390/jof7030231 · Cited by 29 · see citing works
Self-citationReal-time visualization of immune cell clearance of Aspergillus fumigatus spores and hyphae
Knox, B. P., Anna Huttenlocher, A., & Keller, N. P. (2017) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2017.05.005 · Cited by 26 · see citing works
Innate Lung Defense during Invasive Aspergillosis: New Mechanisms
Garth, J. M., & Steele, C. H. (2017) · Journal of Innate Immunity · doi:10.1159/000455125 · Cited by 28 · see citing works
Koch, B. E. V., Hajdamowicz, N. H., Lagendijk, E. L., Ram, A. F. J., & Meijer, A. H. (2019) · Scientific Reports · doi:10.1038/s41598-019-49284-w · Cited by 25 · see citing works
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Feldman, M. B., Vyas, J. M., & Mansour, M. K. (2018) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2018.04.008 · Cited by 25 · see citing works
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Targeting necroptosis: a promising avenue for respiratory disease treatment
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Melanin triggers antifungal defences
Casadevall, A. (2018) · Nature · doi:10.1038/d41586-018-02370-x · Cited by 20 · see citing works
Ortiz, S. C., Pennington, K., Thomson, D. D., & Bertuzzi, M. (2022) · Journal of Fungi · doi:10.3390/jof8030264 · Cited by 21 · see citing works
Illuminating Macrophage Contributions to Host-Pathogen Interactions In Vivo : the Power of Zebrafish
Rosowski, E. E. (2020) · Infection and Immunity · doi:10.1128/iai.00906-19 · Cited by 17 · see citing works
Host defense mechanisms against Aspergillus fumigatus lung colonization and invasion
Mackel, J. J., & Steele, C. H. (2019) · Current Opinion in Microbiology · doi:10.1016/j.mib.2019.04.003 · Cited by 17 · see citing works
Pan, H.-Y., Ladd, A. V., Biswal, M. R., & Valapala, M. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22168684 · Cited by 16 · see citing works
“We've got to get out”—Strategies of human pathogenic fungi to escape from phagocytes
Sonnberger, J., Kasper, L., Lange, T., Brunke, S., & Hube, B. (2023) · Molecular Microbiology · doi:10.1111/mmi.15149 · Cited by 14 · see citing works
Calcineurin inhibitors reduce NFAT-dependent expression of antifungal pentraxin-3 by human monocytes
Bendíčková, K., Tidu, F., Zuani, M. D., Kohoutková, M. H., Andrejčinová, I., Pompeiano, A., Bělašková, S., Forte, G., Zelante, T., & Frič, J. (2019) · Journal of Leukocyte Biology · doi:10.1002/jlb.4vma0318-138r · Cited by 14 · see citing works
Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions
Thrikawala, S. U., & Rosowski, E. E. (2020) · Journal of Visualized Experiments · doi:10.3791/61165 · Cited by 14 · see citing works
Ancuceanu, R., Hovaneț, M. V., Maria, C.-T., Anghel, A.-I., & Dinu, M. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232012543 · Cited by 13 · see citing works
From phagocytosis to metaforosis: Calcineurin’s deadly role in innate processing of fungi
Armstrong-James, D. P. H., de Boer, L. L., Bercusson, A., & Shah, A. (2018) · PLoS Pathogens · doi:10.1371/journal.ppat.1006627 · Cited by 13 · see citing works
Self-citationForn‐Cuní, G., Welvaarts, L., Stel, F., van den Hondel, C. A. M. J. J., Arentshorst, M., Ram, A. F. J., & Meijer, A. H. (2022) · Autophagy · doi:10.1080/15548627.2022.2090727 · Cited by 10 · see citing works
Predicting bladder cancer survival with high accuracy: insights from MAPK pathway-related genes
Cheng, G., Zhou, Z., Li, S., Yang, S., Wang, Y., Ye, Z., & Ren, C. (2024) · Scientific Reports · doi:10.1038/s41598-024-61302-0 · Cited by 11 · see citing works
Sahu, P., & Katwala, J. (2022) · Current Fungal Infection Reports · doi:10.1007/s12281-022-00444-y · Cited by 5 · see citing works
Vehreschild, J. J., Koehler, P. A., Lamoth, F., Prattes, J., Rieger, C. T., Rijnders, B. J. A., & Teschner, D. (2020) · Medical Mycology · doi:10.1093/mmy/myaa079 · Cited by 7 · see citing works
Neuroinflammation in fungal infections: from pathogen recognition to pathological manifestations
Dangarembizi, R., Awala, A. N., & de Lange, A. (2025) · Disease Models & Mechanisms · doi:10.1242/dmm.052344 · Cited by 6 · see citing works
Models for Studying Fungal Pathogenesis
Curtis, A., Oladimeji, F., & Kavanagh, K. A. (2025) · Current Clinical Microbiology Reports · doi:10.1007/s40588-025-00249-w · Cited by 4 · see citing works
Bercusson, A., Williams, T. J., Simmonds, N. J., Alton, E. W. F. W., Uta Griesenbach, D., Shah, A., Warris, A., & Armstrong-James, D. P. H. (2025) · PLoS Pathogens · doi:10.1371/journal.ppat.1012784 · Cited by 4 · see citing works
Self-citationSun, Y., Cui, X., & Zhang, Y. (2025) · Journal de Mycologie Médicale · doi:10.1016/j.mycmed.2025.101598 · Cited by 2 · see citing works
Adlakha, A., Williams, T. J., Shou, X., Reed, A. K., Lenhard, B., & Armstrong-James, D. P. H. (2024) · iScience · doi:10.1016/j.isci.2024.111535 · Cited by 2 · see citing works
Self-citationLv, J.-X., Pei, Y.-Y., Yang, C., Liu, X., Ju, M., Holmes, E. C., Chen, Y., Zhu, T., & Zhang, Y. (2025) · PLoS Pathogens · doi:10.1371/journal.ppat.1013667 · Cited by 2 · see citing works
Combined Effect of Platelets and Anidulafungin against Aspergillus fumigatus Infections
Schiefermeier Mach, N., Bellmann, R., Fréalle, É., & Perkhofer, S. (2017) · Journal of Infectious Diseases and Therapy · doi:10.4172/2332-0877.1000334 · Cited by 2 · see citing works
He, H., Cai, L., Liu, Y., Lin, Y., Zhu, X., Xue, X., & Pan, W. (2024) · Discover Life · doi:10.1007/s11084-024-09676-z · Cited by 2 · see citing works
Seyedmousavi, S., & Davis, M. J. (2017) · Virulence · doi:10.1080/21505594.2017.1380143 · Cited by 2 · see citing works
Allergic Bronchopulmonary Aspergillosis
Chan, L., Nwankwo, L., & Shah, A. (2021) · Elsevier eBooks · doi:10.1016/b978-0-08-102723-3.00200-6 · Cited by 2 · see citing works
Self-citationKundu, S., Riegler, A. N., Chelappan, R., Mishra, D. K., Dave, T. V., Leal, S. M., & Roy, S. (2025) · Medical Mycology · doi:10.1093/mmy/myaf104 · Cited by 1 · see citing works
Z-Nucleic Acids: A Regulator of Macrophage PANoptosis and Fungal Biofilm in Fungal Keratitis
Gao, N., Ma, Y., Zhang, M., Cui, M., Li, X., & Yang, R. (2026) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.67.10.6 · Cited by 1 · see citing works
Pulmonary Disease in the Pediatric Patient With Acquired Immunodeficiency States
Spahr, J. E., Weiner, D. J., Stokes, D. C., & Kurland, G. (2019) · Elsevier eBooks · doi:10.1016/b978-0-323-44887-1.00064-x · Cited by 1 · see citing works
Developing novel approaches to treat trauma-associated fungal infections
Trzaska, W. J. (2019) · University of Birmingham Institutional Research Archive (University of Birmingham) · Cited by 1 · see citing works
Host-Induced Stress Response in Human Pathogenic Fungi
Viana, R., Pais, P., Cavalheiro, M., Galocha, M., & Teixeira, M. C. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-819990-9.00035-4 · Cited by 1 · see citing works
Investigating the molecular mechanisms of vomocytosis
Gilbert, A. S. (2017) · University of Birmingham Institutional Research Archive (University of Birmingham)
Bercusson, A. (2019) · Spiral (Imperial College London) · doi:10.25560/82028
Jia, L., Rafiq, M., Radosa, L., Hortschansky, P., Cunha, C., Cseresnyés, Z., Krüger, T., Schmidt, F., Heinekamp, T., Straßburger, M., et al. (2022) · SSRN Electronic Journal · doi:10.2139/ssrn.4158007
Status of Ethiopian durum wheat varieties for the stem rust disease resistance under field condition
Ayehu, H. T., Temtme, M., & Wasihun, L. (2022) · African Journal of Agricultural Research · doi:10.5897/ajar2022.16136
Bercusson, A., Williams, T. J., Simmonds, N. J., Alton, E. W. F. W., Uta Griesenbach, D., Shah, A., Warris, A., & Armstrong-James, D. P. H. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.11.29.625998
Self-citationLi, M., Huang, H., Li, D., Zheng, J., Liu, Y., Chen, Y., Xie, Z., Xi, L., & Liu, H. (2025) · PLoS neglected tropical diseases · doi:10.1371/journal.pntd.0013816
The role of calcium ions and the calcineurin route in fungal virulence: a review
de Andrade, F. P., da Silva, G. R., de Sousa, V. D., & Costa, B. A. (2026) · Revista Colombiana de Ciencias Químico Farmacéuticas · doi:10.15446/rcciquifa.v55n1.125068
- Authors
- Shah, A., Kannambath, S., Herbst, S., Rogers, A., Soresi, S., Carby, M., Reed, A., Mostowy, S., Fisher, M. C., Shaunak, S., & Armstrong-James, D. P.
- Affiliations
- Imperial College London (GB)
- Royal Brompton & Harefield NHS Foundation Trust (GB)
- Corresponding author
- Armstrong-James, Darius P.
- ORCID
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-11-01
- Volume
- 194
- Issue
- 9
- Pages
- 1127-1139
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- PMC
- PMC5114448
- OpenAlex
- W2359912528
- Cited by
- 71
- References
- 48
- Citations by year
- 2026: 2 · 2025: 8 · 2024: 6 · 2023: 4 · 2022: 10 · 2021: 5 · 2020: 7 · 2019: 9 · 2018: 9 · 2017: 11
- FWCI
- 8.87
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- American Journal of Respiratory and Critical Care Medicine (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- St George's Online Research Archive (St George's University of London) (acceptedVersion)
- LSHTM Research Online (London School of Hygiene and Tropical Medicine) (acceptedVersion)
- Spiral (Imperial College London) (submittedVersion)
- Funders
- Medical Research Council (G0902260/1, MR/K002708/1)
- Wellcome Trust Research Career Development Fellowship (WT097411MA)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Aspergillus fumigatus, pulmonary aspergillosis, calcineurin, FK506, tacrolimus, calcineurin inhibitors, lateral transfer, fungal spore germination, phagosome, late endosomes, VASP, immunosuppression, transplant recipients, zebrafish model
- MeSH terms
- Aspergillus fumigatus, Humans, Macrophages, Necrosis, Phagocytosis, Spores, Fungal, Cell Death, Reactive Oxygen Species, Calcineurin, MAP Kinase Signaling System, Pulmonary Aspergillosis
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