The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Toll-Like Receptors in Normal and Cystic Fibrosis Airway Epithelial Cells
Abstract
Toll-like receptors (TLRs) mediate cellular responses to diverse microbial ligands. The distribution and function of TLRs in airway cells were studied to identify which are available to signal the presence of inhaled pathogens and to establish if differences in TLR expression are associated with the increased proinflammatory responses seen in cystic fibrosis (CF). Isogenic, polarized CF and control bronchial epithelial cell lines, human airway cells in primary culture, and cftr null and wild-type mice were compared. TLRs 1-10, MD2, and MyD88 were expressed in CF and normal cells. Only TLR2 transcription was modestly increased in CF as compared with normal epithelial cells following bacterial stimulation. TLR2 was predominantly at the apical surface of airway cells and was mobilized to cell surface in response to bacteria. TLR4 was present in a more basolateral distribution in airway cells, but appeared to have a limited role in epithelial responses. Lipopolysaccharide failed to activate nuclear factor-kappaB in these cells, and TLR2 dominant negative but not TLR4 dominant negative mutants inhibited activation by both Gram-negative and Gram-positive bacteria. Increased availability of TLR2 at the apical surfaces of CF epithelial cells is consistent with the increased proinflammatory responses seen in CF airways and suggests a selective participation of TLRs in the airway mucosa.
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Cites
Toll-like receptors: critical proteins linking innate and acquired immunity
Akira, S., Takeda, K., & Kaisho, T. (2001) · Nature Immunology · doi:10.1038/90609 · Cited by 4,777
The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5
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Toll-like receptors in the induction of the innate immune response
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Human TLR9 confers responsiveness to bacterial DNA via species-specific CpG motif recognition
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Cited by
TLR4 and CD14 trafficking and its influence on LPS-induced pro-inflammatory signaling
Ciesielska, A., Matyjek, M., & Kwiatkowska, K. (2021) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-020-03656-y · Cited by 1,420
Rowe, S. M., Miller, S., & Sorscher, E. J. (2005) · New England Journal of Medicine · doi:10.1056/nejmra043184 · Cited by 1,398
The airway epithelium: soldier in the fight against respiratory viruses
Vareille, M., Kieninger, E., Edwards, M. R., & Regamey, N. (2011) · Clinical Microbiology Reviews · doi:10.1128/cmr.00014-10 · Cited by 655
The role of the microbiome in exacerbations of chronic lung diseases
Dickson, R. P., Martinez, F. J., & Huffnagle, G. B. (2014) · The Lancet · doi:10.1016/s0140-6736(14)61136-3 · Cited by 495
Bacterial flagellins: mediators of pathogenicity and host immune responses in mucosa
Ramos, H. C., Rumbo, M., & Sirard, J.-C. (2004) · Trends in Microbiology · doi:10.1016/j.tim.2004.09.002 · Cited by 485
Metadata
- Authors
- Muir, A., Soong, G., Sokol, S., Reddy, B., Gomez, M. I., van Heeckeren, A., & Prince, A.
- Venue
- American Journal of Respiratory Cell and Molecular Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-06
- Volume
- 30
- Issue
- 6
- Pages
- 777-783
- ISSN
- 1044-1549 (ISSN-L)
- 1535-4989
- OpenAlex
- W2000229886
- Cited by
- 259
- References
- 43
- FWCI
- 5.72
- Type
- journal article
- Language
- en
- Open access
- closed
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- Muir, A., Soong, G., Sokol, S., Reddy, B., Gomez, M. I., van Heeckeren, A., & Prince, A. (2004). Toll-Like Receptors in Normal and Cystic Fibrosis Airway Epithelial Cells. American Journal of Respiratory Cell and Molecular Biology. https://doi.org/10.1165/rcmb.2003-0329oc
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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