Polymeric nanocarriers for non-viral gene delivery
Abstract
Gene therapy holds great promise for the treatment of acquired and inherited diseases. Non-viral gene delivery systems are gaining recognition as alternatives to viral gene vectors due to their safety and low immunogenicity. The effective delivery of nucleic acids requires overcoming many biological barriers that would otherwise hinder transfection efficiency. Developing safe and efficient vectors that can overcome these obstacles is at the heart of current gene delivery research. Polymeric nanocarriers, such as polyethylenimine (PEI), poly(L-lysine) (PLL), poly[2-(dimethylamino) ethyl methacrylate] (PDMAEMA), polyamidoamine (PAMAM), chitosan and poly(amino-co-ester)s (PAEs), play an important role in gene delivery. In this review, we first describe the current understanding of the biological barriers to non-viral gene delivery and recent progress in addressing these barriers. Several of the most widely used non-viral polymeric nanocarriers are discussed, and detailed strategies that have accumulated from nearly two decades of research aimed at overcoming biological barriers and improving gene transfer are highlighted.
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Cationic non-viral vectors used to condense negatively charged CRISPR RNPs and plasmids are prone to interact with plasma proteins and physiological salt, resulting in recognition and clearance by RES.
“On the other hand, cationic non-viral vectors used to condense negatively charged CRISPR RNPs and plasmids are prone to interact with plasma proteins and the physiological salt in physiological conditions, resulting in the recognition and clearance by the reticuloendothelial system (RES) [141–143].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [141]
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Lee, J. R., Kim, Y., Kim, E., et al. (2024) · Biomaterials Research · doi:10.34133/bmr.0087 · Cited by 2 · see citing works
“Nonviral gene delivery systems are typically composed of genes condensed into nanoparticles pre pared with cationic polymers such as polylysine, polyethylenei mine (PEI), polyamidoamine, chitosan, and dendrimers [13,14].”
BackgroundPolypiperazine-Based Micelles of Mixed Composition for Gene Delivery
Stancheva, R., Haladjova, E., Petrova, M., Ugrinova, I., Dimitrov, I. V., & Rangelov, S. (2024) · Polymers · doi:10.3390/polym16213100 · Cited by 2 · see citing works
Dendrimer-based nanomedicines for cancer immunotherapy
Azghandi, M. V., Mollazadeh, S., Ghaemi, A., Ramezani, M., & Alibolandi, M. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-18770-4.00003-2 · Cited by 2 · see citing works
Halagali, P., Seenivasan, R., Sreedevi, A., Ligade, V. S., Bugadannavar, P., Sharma, H., Nand, S., & Tippavajhala, V. K. (2026) · 3 Biotech · doi:10.1007/s13205-026-05016-2 · Cited by 2 · see citing works
“Yet, achieving a balance between killing the immune system and delivering the gene remains a complex task (Zhou et al. 2004; Hosseinkhani et al. 2015; Li et al. 2015).”
- Authors
- Li, L., Wei, Y., & Gong, C.
- Venue
- Journal of Biomedical Nanotechnology
- Publisher
- American Scientific Publishers
- Published
- 2015-05-01
- Volume
- 11
- Issue
- 5
- Pages
- 739-770
- ISSN
- 1550-7033 (ISSN-L)
- OpenAlex
- W2054359043
- Cited by
- 106
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 8 · 2023: 7 · 2022: 8 · 2021: 15 · 2020: 9 · 2019: 11 · 2018: 12 · 2017: 11 · 2016: 16 · 2015: 6
- FWCI
- 4.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Biomedical Nanotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- non-viral gene delivery, polymeric nanocarriers, polyethyleneimine, poly-L-lysine, PDMAEMA, PAMAM, chitosan, gene transfer efficiency, gene therapy vectors
- MeSH terms
- Animals, Humans, Nucleic Acids, Polymers, Transfection, Genetic Therapy, Chitosan, Nanoparticles
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