Attenuated release of biologically active luteinizing hormone in healthy aging men.
Open accessAbstract
To examine the biological quality and physiologically pulsatile mode of endogenous luteinizing hormone release in active, healthy aging men, we used the rat interstitial-cell testosterone in vitro bioassay to probe LH bioactivity in response to (a) endogenous gonadotropin-releasing hormone (GnRH) action (basal pulsatile bioactive LH secretion); (b) exogenous GnRH stimulation (10 micrograms IV pulses); and (c) inhibition of endogenous estrogen negative feedback (treatment with a nonsteroidal antiestrogen, tamoxifen). Basally, some healthy older men exhibited evidence of neuroendocrine dysfunction, reflected by irregular bursts of bioactive LH release followed by transiently low plasma bio:immuno (B:I) LH ratios. However, mean basal plasma bioactive LH concentrations, B:I ratios, and spontaneous LH pulse properties (peak frequency, amplitude, duration, and enhanced B:I ratios within LH peaks) were not altered in older men. On the other hand, augmentation of bioactive LH secretion and enhancement of plasma B:I ratios by pulsed injections of exogenous GnRH were either significantly reduced or absent in older men. In addition, although tamoxifen increased bioactive LH pulse frequency in both age groups and facilitated exogenous GnRH action in some subjects, older men increased their 12-h mean bioactive LH concentrations, B:I ratios, and bioactive LH peak amplitudes to a significantly lesser degree than young men. In summary, young and older healthy men exhibit similar mean basal plasma bioactive LH concentrations and spontaneous LH pulse properties. However, pituitary bioactive LH reserve is markedly attenuated in older men challenged with either exogenous GnRH or antiestrogen. Accordingly, we conclude that healthy aging men manifest an impaired secretory reserve for biologically active LH release.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Experimental studies demonstrate reduced LH and testosterone secretion in response to SERMs or AIs in older compared to young men.
“Experimental studies demonstrate reduced LH and testosterone secretion in response to SERMs 27,72 or AIs 107 in older compared to young men,”
From Hypogonadism and male obesity: Focus on unresolved questions (2018) · cites this paper as [27]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Cluster analysis: a simple, versatile, and robust algorithm for endocrine pulse detection
Veldhuis, J. D., & Johnson, M. L. (1986) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.1986.250.4.e486 · Cited by 855 · see citing works
Self-citationVeldhuis, J. D., Carlson, M. L., & Johnson, M. L. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7686 · Cited by 556 · see citing works
Self-citationHormonal Changes and Sexual Function in Aging Men*
Davidson, J. M., Chen, J. J., CRAPO, L., Gray, G. D., GREENLEAF, W. J., & Catania, J. A. (1983) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-57-1-71 · Cited by 377 · see citing works
CHANGES IN THE PITUITARY‐TESTICULAR SYSTEM WITH AGE
Baker, H. W. G., Burger, H., KRETSER, D. M. D., Hudson, B., O'connor, S., Wang, C.-L., MIROVICS, A., Court, J. M., Dunlop, M., & RENNIE, G. C. (1976) · Clinical Endocrinology · doi:10.1111/j.1365-2265.1976.tb01964.x · Cited by 305 · see citing works
InVitroBioassay of LH in Human Serum: The Rat Interstitial Cell Testosterone (RICT) Assay
Dufau, M. L., POCK, R., NEUBAUER, A., & Catt, K. J. (1976) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-42-5-958 · Cited by 174 · see citing works
Self-citationDeslypère, J. P., Kaufman, J., VERMEULEN, T., Vogelaers, D. P., Vandalem, J. L., & Vermeulen, A. (1987) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-64-1-68 · Cited by 159 · see citing works
Zumoff, B., Strain, G. W., Kream, J., O'CONNOR, J., Rosenfeld, R. S., Levin, J., & Fukushima, D. K. (1982) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-54-3-534 · Cited by 143 · see citing works
Metabolic clearance of biologically active luteinizing hormone in man.
Veldhuis, J. D., FRAIOLI, F., Rogol, A. D., & Dufau, M. L. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112411 · Cited by 130 · see citing works
Self-citationHarman, S. M., Tsitouras, P. D., Costa, P. T., & Blackman, M. R. (1982) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-54-3-547 · Cited by 116 · see citing works
Warner, B. A., Worgul, T. J., Drago, J. R., et al. (1983) · Journal of Clinical Investigation · doi:10.1172/jci110940 · Cited by 105 · see citing works
Dufau, M. L., Beitins, I. Z., McArthur, J. W., & Catt, K. J. (1976) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-43-3-658 · Cited by 104 · see citing works
Self-citationVeldhuis, J. D., Beitins, I. Z., Johnson, M. L., et al. (1984) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-58-6-1050 · Cited by 99 · see citing works
Self-citationWarner, B. A., Dufau, M. L., & Santen, R. J. (1985) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-60-2-263 · Cited by 90 · see citing works
Self-citationDufau, M. L., HODGEN, S. D., Goodman, A. L., & Catt, K. J. (1977) · Endocrinology · doi:10.1210/endo-100-6-1557 · Cited by 89 · see citing works
Self-citationEstradiol modulates the pulsatile secretion of biologically active luteinizing hormone in man.
Veldhuis, J. D., & Dufau, M. L. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113115 · Cited by 81 · see citing works
Self-citationVeldhuis, J. D., Rogol, A. D., & Johnson, M. L. (1983) · Journal of Clinical Investigation · doi:10.1172/jci111168 · Cited by 80 · see citing works
Self-citationMode of Secretion of Bioactive Luteinizing Hormone in Man*
Dufau, M. L., Veldhuis, J. D., FRAIOLI, F., Johnson, M. L., & Beitins, I. Z. (1983) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-57-5-993 · Cited by 80 · see citing works
Self-citationPathophysiology of Male Hypogonadism Associated with Endogenous Hyperestrogenism
Veldhuis, J. D., Sowers, J. R., Rogol, A. D., Klein, F. A., Miller, N. E., & Dufau, M. L. (1985) · New England Journal of Medicine · doi:10.1056/nejm198505233122107 · Cited by 78 · see citing works
Self-citationDufau, M. L., & Veldhuis, J. D. (1987) · Baillière s Clinical Endocrinology and Metabolism · doi:10.1016/s0950-351x(87)80057-5 · Cited by 76 · see citing works
Self-citationDiminished Luteinizing Hormone Pulse Frequency and Amplitude with Aging in the Male Rat*
Karpas, A. E., Bremner, W. J., Clifton, D. K., et al. (1983) · Endocrinology · doi:10.1210/endo-112-3-788 · Cited by 64 · see citing works
Reduced Pulsatile Luteinizing Hormone and Testosterone Secretion with Aging in the Male Rat 1
Steiner, R. A., Bremner, W. J., Clifton, D. K., et al. (1984) · Biology of Reproduction · doi:10.1095/biolreprod31.2.251 · Cited by 63 · see citing works
Veldhuis, J. D., Johnson, M. L., & Dufau, M. L. (1987) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-64-6-1275 · Cited by 53 · see citing works
Self-citationRelation of neuroendocrine system to the reproductive decline in aging rats and human subjects.
Meites, J., Rw, S., & Hh, H. (1980) · PubMed · Cited by 43 · see citing works
Beitins, I. Z., Dufau, M. L., O’Loughlin, K. G., Catt, K. J., & McArthur, J. W. (1977) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-45-3-605 · Cited by 36 · see citing works
Self-citationHypophyseal-gonadal system during male aging
Moroz, E. V., & Verkhratsky, N. S. (1985) · Archives of Gerontology and Geriatrics · doi:10.1016/0167-4943(85)90013-5 · Cited by 28 · see citing works
In VitroRelease of Luteinizing Hormone-Releasing Hormone from the Hypothalamus of Old Male Rats*
ZANISI, M., Messi, E., & Martini, L. (1987) · Endocrinology · doi:10.1210/endo-120-1-49 · Cited by 18 · see citing works
Elevated serum follicle-stimulating hormone levels in men with normal seminal fluid analyses
Karpas, A. E., Matsumoto, A. M., Paulsen, C. A., et al. (1983) · Fertility and Sterility · doi:10.1016/s0015-0282(16)46881-0 · Cited by 16 · see citing works
Celani, M. F., Montanini, V., Baraghini, G. F., Carani, C., & Marrama, P. (1984) · Journal of Endocrinological Investigation · doi:10.1007/bf03349491 · Cited by 12 · see citing works
Peripubertal changes in the nature of LH
Buckingham, J. C., & WILSON, C. A. (1985) · Journal of Endocrinology · doi:10.1677/joe.0.1040173 · Cited by 9 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- H. Burger, editor. W. B. Saunders, Philadelphia. 153-176.
Cited by
Sorted by citations
Andropause: clinical implications of the decline in serum testosterone levels with aging in men
Matsumoto, A. M. (2002) · The Journals of Gerontology Series A: Biological Sciences and Medical Sciences · doi:10.1093/gerona/57.2.m76 · Cited by 490 · see citing works
Fink, G., Sumner, B., Rosie, R., Wilson, H., & McQueen, J. (1999) · Behavioural Brain Research · doi:10.1016/s0166-4328(99)00082-0 · Cited by 198 · see citing works
Declining gonadal function in elderly men
Kaufman, J. M., & Vermeulen, A. (1997) · Baillière s Clinical Endocrinology and Metabolism · doi:10.1016/s0950-351x(97)80302-3 · Cited by 181 · see citing works
Hypogonadism and male obesity: Focus on unresolved questions
Grossmann, M. (2018) · Clinical Endocrinology · doi:10.1111/cen.13723 · Cited by 164 · see citing works
Mulligan, T., Iranmanesh, A., Kerzner, R., Demers, L., & Veldhuis, J. (1999) · European Journal of Endocrinology · doi:10.1530/eje.0.1410257 · Cited by 121 · see citing works
Self-citationGolan, R., Scovell, J. M., & Ramasamy, R. (2015) · The Aging Male · doi:10.3109/13685538.2015.1052392 · Cited by 73 · see citing works
Veldhuis, J. D., Liu, P. Y., Keenan, D. M., & Takahashi, P. Y. (2012) · American Journal of Physiology-Endocrinology and Metabolism · doi:10.1152/ajpendo.00450.2011 · Cited by 48 · see citing works
Self-citationVeldhuis, J. D., & Iranmanesh, A. (2005) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2004-0834 · Cited by 36 · see citing works
Self-citation
- Authors
- Urban, R. J., Veldhuis, J. D., Blizzard, R. M., & Dufau, M. L.
- Affiliations
- University of Virginia Medical Center (US)
- National Institutes of Health (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1988-04-01
- Volume
- 81
- Issue
- 4
- Pages
- 1020-1029
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC329626
- OpenAlex
- W1980911368
- Cited by
- 93
- References
- 29
- Citations by year
- 2025: 1 · 2024: 2 · 2022: 2 · 2021: 1 · 2020: 1 · 2018: 2 · 2017: 2 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 6.11
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Virginia
- National Institutes of Health (5k04hd000634-02, 1 K04 HD 00634)
- National Institute on Aging (AG04303-03)
- National Center for Research Resources (RR 00847)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (K04 HD 00634)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
- Keywords
- luteinizing hormone, LH bioactivity, pulsatile LH secretion, GnRH stimulation, tamoxifen, estrogen negative feedback, neuroendocrine dysfunction
- MeSH terms
- Aged, Aging, Biological Assay, Estradiol, Humans, Luteinizing Hormone, Gonadotropin-Releasing Hormone, Male, Middle Aged, Periodicity, Radioimmunoassay, Secretory Rate, Tamoxifen, Testosterone
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
