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Cited by 360

The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.

Inhibition of Ca(2+)-dependent K+ transport and cell dehydration in sickle erythrocytes by clotrimazole and other imidazole derivatives.

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Brugnara, C, de Franceschi, L, Alper, S L·1993·Journal of Clinical Investigation
DOI 10.1172/jci116597
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Abstract

We have investigated the interaction of clotrimazole (CLT) and related compounds with the erythroid Ca(2+)-activated K+ channel, a mediator of sickle cell dehydration. We measured K+ transport, membrane potential, and cell volume upon activation of this pathway in sickle erythrocytes. CLT blocked almost completely Ca(2+)-activated K+ transport in homozygous hemoglobin S cells, with IC50 values of 29 +/- 15 nM in isotonic 20 mM salt solution and 51 +/- 15 nM in normal saline (n = 3). The inhibition of K+ transport by CLT was caused by a specific interaction with the Ca(2+)-activated K+ channel of human red cells, since it displaced bound 125I-Charybdotoxin, a specific ligand of the Gardos channel, with an IC50 (12 +/- 4 nM in isotonic 20 mM) similar to the IC50 values for flux inhibition. When homozygous hemoglobin S cells were dehydrated by incubation in the presence of 100 microM CaCl2 and the ionophore A23187, or by exposure to cycles of oxygenation and deoxygenation, CLT effectively inhibited cell dehydration and K+ loss. The IC50 of CLT for inhibition of Ca(2+)-activated K+ transport in sickle cells is significantly lower than plasma concentrations of CLT achievable after nontoxic oral doses. We therefore propose that oral administration of CLT may prevent red cell dehydration in patients with sickle cell anemia.

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Metadata

Authors
Brugnara, C., de Franceschi, L., & Alper, S. L.
Venue
Journal of Clinical Investigation
Publisher
American Society for Clinical Investigation
Published
1993-07-01
Volume
92
Issue
1
Pages
520-526
ISSN
0021-9738 (ISSN-L)
DOI
10.1172/jci116597
OpenAlex
W1976466808
Cited by
360
References
17
FWCI
6.60
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Inhibition of Ca(2+)-dependent K+ transport and cell dehydration in sickle erythrocytes by clotrimazole and other imidazole derivatives. https://app.cochat.ai/papers/10.1172/jci116597
The paper
Brugnara, C., de Franceschi, L., & Alper, S. L. (1993). Inhibition of Ca(2+)-dependent K+ transport and cell dehydration in sickle erythrocytes by clotrimazole and other imidazole derivatives. Journal of Clinical Investigation. https://doi.org/10.1172/jci116597

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.