The DOI matches its Crossref record.
Molecular cloning, chromosomal localization, and functional characterization of a human liver Na+/bile acid cotransporter.
Open accessAbstract
We have used a cDNA probe from a cloned rat liver Na+/taurocholate cotransporting polypeptide (Ntcp) to screen a human liver cDNA library. A 1,599-bp cDNA clone that encodes a human Na+/taurocholate cotransporting polypeptide (NTCP) was isolated. The human NTCP consists of 349 amino acids (calculated molecular mass of 38 kD) and exhibits 77% amino acid homology with the rat Ntcp. In vitro translation experiments indicate that the protein is glycosylated and has a molecular weight similar to the rat Ntcp. Injection of in vitro transcribed cRNA into Xenopus laevis oocytes resulted in the expression of Na(+)-dependent taurocholate uptake. Saturation kinetics indicated that the human NTCP has a higher affinity for taurocholate (apparent Km = 6 microM) than the previously cloned rat protein (apparent Km = 25 microM). NTCP-mediated taurocholate uptake into oocytes was inhibited by all major bile acid derivatives (100 microM), bumetanide (500 microM), and bromosulphophthalein (100 microM). Southern blot analysis of genomic DNA from a panel of human/hamster somatic cell hybrids mapped the human NTCP gene to chromosome 14.
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Metadata
- Authors
- Hagenbuch, B., & Meier, P. J.
- Venue
- Journal of Clinical Investigation
- Published
- 1994-03-01
- Volume
- 93
- Issue
- 3
- Pages
- 1326-1331
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2105517464
- Cited by
- 464
- References
- 0
- FWCI
- 3.80
- Type
- journal article
- Language
- en
- Open access
- bronze
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