The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Order and specificity of the Plasmodium falciparum hemoglobin degradation pathway.
Open accessAbstract
The human malaria parasite, Plasmodium falciparum, degrades nearly all its host cell hemoglobin during a short segment of its intraerythrocytic development. This massive catabolic process occurs in an acidic organelle, the digestive vacuole. Aspartic and cysteine proteases have been implicated in this pathway. We have isolated three vacuolar proteases that account for most of the globin-degrading activity of the digestive vacuole. One is the previously described aspartic hemoglobinase that initiates hemoglobin degradation. A second aspartic protease is capable of cleaving hemoglobin with an overlapping specificity, but seems to prefer acid-denatured globin. The third is a cysteine protease that does not recognize native hemoglobin but readily cleaves denatured globin. It is synergistic with the aspartic hemoglobinase, both by in vitro assay of hemoglobin degradation, and by isobologram analysis of protease inhibitor-treated parasites in culture. The cysteine protease is highly sensitive to chloroquine-heme complex, suggesting a possible mechanism of 4-aminoquinoline antimalarial action. The data suggest an ordered pathway of hemoglobin catabolism that presents an excellent target for chemotherapy.
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Cites
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Cited by
Laing, R., Kikuchi, T., Martinelli, A., Tsai, I. J., Beech, R. N., Redman, E., Holroyd, N., Bartley, D. J., Beasley, H., Britton, C., et al. (2013) · Genome Biology · doi:10.1186/gb-2013-14-8-r88 · Cited by 373
Aspartic proteases of Plasmodium falciparum and other parasitic protozoa as drug targets
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Self-citationAn Antiviral Mechanism of Nitric Oxide
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A Multi-enzyme Cascade of Hemoglobin Proteolysis in the Intestine of Blood-feeding Hookworms
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Metadata
- Authors
- Gluzman, I. Y., Francis, S. E., Oksman, A., Smith, C. E., Duffin, K. L., & Goldberg, D. E.
- Venue
- Journal of Clinical Investigation
- Published
- 1994-04-01
- Volume
- 93
- Issue
- 4
- Pages
- 1602-1608
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2031796366
- Cited by
- 306
- References
- 32
- FWCI
- 20.22
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Gluzman, I. Y., Francis, S. E., Oksman, A., Smith, C. E., Duffin, K. L., & Goldberg, D. E. (1994). Order and specificity of the Plasmodium falciparum hemoglobin degradation pathway. Journal of Clinical Investigation. https://doi.org/10.1172/jci117140
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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