Metabolomics of supragingival plaque and oral bacteria

Takahashi, N.; Washio, J.; Mayanagi, G.

doi:10.1177/0022034510377792

Cited by 107Oral microbiology and periodontitis researchDental Health and Care UtilizationMetabolomics and Mass Spectrometry Studies

Metabolomics of supragingival plaque and oral bacteria

Takahashi, N., Washio, J., Mayanagi, G.·2010·Journal of Dental Research

DOI 10.1177/0022034510377792

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Abstract

Dental caries is initiated by demineralization of the tooth surface through acid production by sugar metabolism of supragingival plaque microflora. To elucidate the sugar metabolic system, we used CE-MS to perform metabolomics of the central carbon metabolism, the EMP pathway, the pentose-phosphate pathway, and the TCA cycle in supra- gingival plaque and representative oral bacteria, Streptococcus and Actinomyces. Supragingival plaque contained all the targeted metabolites in the central carbon metabolism, except erythrose 4-phosphate in the pentose-phosphate pathway. After glucose rinse, glucose 6-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, dihydroxyacetone phosphate, and pyruvate in the EMP pathway and 6-phosphogluconate, ribulose 5-phosphate, and sedoheptulose 7-phosphate in the pentose-phosphate pathway, and acetyl CoA were increased. Meanwhile, 3-phosphoglycerate and phosphoenolpyruvate in the EMP pathway and succinate, fumarate, and malate in the TCA cycle were decreased. These pathways and changes in metabolites observed in supragingival plaque were similar to the integration of metabolite profiles in Streptococcus and Actinomyces.

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Additional file 5 of Salivary microbiome and metabolome analysis of severe early childhood caries
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Authors: Takahashi, N., Washio, J., & Mayanagi, G.
Affiliations: Tohoku University (JP)
ORCID: Takahashi, N.
Washio, J.
Venue: Journal of Dental Research
Publisher: SAGE Publications
Published: 2010-10-05
Volume: 89
Issue: 12
Pages: 1383-1388
ISSN: 0022-0345 (ISSN-L)
1544-0591
DOI: 10.1177/0022034510377792
OpenAlex: W2039105696
Cited by: 107
References: 33
Citations by year: 2026: 7 · 2025: 4 · 2024: 4 · 2023: 8 · 2022: 12 · 2021: 8 · 2020: 9 · 2019: 4 · 2018: 2 · 2017: 4 · 2016: 8 · 2015: 12 · 2014: 6 · 2013: 5 · 2012: 9
FWCI: 7.05
Type: journal article
Language: en
Open access: closed
Licence: journals.sagepub.com/page/policies/text-and-data-mining-license (tdm)
Locations: Journal of Dental Research (publishedVersion)
PubMed (publishedVersion)
Funders: Japan Society for the Promotion of Science (22792104, 22390399)
Topics: Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Health Sciences › Dentistry › Periodontics › Dental Health and Care Utilization
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords: supragingival plaque, oral bacteria, Streptococcus, Actinomyces, sugar metabolism, dental caries, central carbon metabolism, glycolysis, pentose phosphate pathway, TCA cycle, metabolite profiling, acid production
MeSH terms: Acetyl Coenzyme A, Actinomyces, Adult, Bacteriological Techniques, Carbon, Citric Acid Cycle, Dental Plaque, Dihydroxyacetone Phosphate, Female, Fructosediphosphates, Fructosephosphates, Fumarates, Gluconates, Glucose, Glyceric Acids, Glycolysis, Humans, Malates, Male, Pentose Phosphate Pathway, Phosphoenolpyruvate, Ribulosephosphates, Streptococcus, Streptococcus mutans, Sugar Phosphates, Pyruvic Acid, Glucose-6-Phosphate, Succinic Acid, Metabolomics

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Metabolomics of supragingival plaque and oral bacteria

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