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Evaluation of Cell Proliferation in Rat Tissues with BrdU, PCNA, Ki-67(MIB-5) Immunohistochemistry and In Situ Hybridization for Histone mRNA
Abstract
The standard method for assessment of cell proliferation in paraffin-embedded tissue sections is 5-bromodeoxyuridine (BrdU) immunohistochemistry (IHC). BrdU can be administered to laboratory animals via IP injections, is readily incorporated into nuclei during the DNA synthetic phase of the cell cycle, and is detected with an anti-BrdU antibody. This method has several disadvantages, and an accurate method for evaluation of proliferative activity that can substitute for BrdU IHC, when necessary, is of great interest to investigators. Alternative methods for detection of proliferating cells in tissue sections are proliferating cell nuclear antigen (PCNA) IHC, Ki-67 IHC, and in situ hybridization (ISH) for histone mRNA. To determine the optimal choice, we analyzed the correlation of anti-PCNA, anti-Ki-67(MIB-5), and histone mRNA labeling indices (LIs) with anti-BrdU LI in rat highly replicative (renewing) tissues. The correlation between anti-BrdU and histone mRNA LIs, as well as the correlation between anti-BrdU and anti-Ki-67 LIs, was statistically significant. There was no significant correlation between anti-BrdU and anti-PCNA LIs. These results suggest that both ISH for histone mRNA and IHC with MIB-5 are preferable techniques for assessment of cell proliferation in rat paraffin-embedded renewing tissues compared to PCNA IHC. They can substitute for BrdU IHC when necessary.
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Cites
Gerdes, J., Lemke, H., Baisch, H., Wacker, H. H., Schwab, U., & Stein, H. (1984) · The Journal of Immunology · doi:10.4049/jimmunol.133.4.1710 · Cited by 4,173
Research works printed but not linked: 58
- 10.1177/096032719501401201 · doi:10.1177/096032719501401201
- 10.1177/42.12.7983360 · doi:10.1177/42.12.7983360
- 10.1016/S0002-9440(10)64670-6 · DOI
- 10.1038/326515a0 · DOI · Paper page
- 10.1083/jcb.105.4.1549 · DOI · Paper page
- 10.1111/j.1365-2184.1992.tb01435.x · DOI · Paper page
- Budke H, 1994, Mod Pathol, 7, 860
- 10.1016/0014-5793(86)81127-9 · DOI · Paper page
- 10.1177/39.1.1670579 · DOI · Paper page
- 10.1177/153537020122601114 · DOI
- Dervan PA, 1992, Am J Clin Pathol, 97, S21, 10.1093/ajcp/97.1.21 · DOI
- Dijkman HB, 1996, Nonradioactive In Situ Hybridization Application Manual, 152
- 10.1006/faat.1996.0119 · DOI
- 10.1007/s005340170025 · doi:10.1007/s005340170025
- 10.1038/sj.leu.2402540 · DOI
- Fedrowitz M, 2002, Cancer Res, 62, 1356
- 10.1289/ehp.93101s5199 · DOI
- 10.1016/S0304-3835(99)00191-3 · DOI
- Gerlach C, 1997, Lab Invest, 77, 697
- 10.1002/hep.510260307 · DOI
- 10.1097/00000478-199501000-00009 · DOI
- Goldsworthy TL, 1991, Chemically-induced Cell Proliferation: Implications for Risk Assessment, 253
- 10.1177/44.3.8648081 · doi:10.1177/44.3.8648081
- 10.1126/science.7123245 · DOI · Paper page
- 10.1002/path.1711620403 · DOI · Paper page
- 10.1111/j.1349-7006.2001.tb01109.x · doi:10.1111/j.1349-7006.2001.tb01109.x
- 10.1128/MCB.3.4.539 · DOI · Paper page
- 10.1007/s004180050321 · doi:10.1007/s004180050321
- 10.1136/jcp.44.8.655 · doi:10.1136/jcp.44.8.655
- Kato T, 2002, Anticancer Res, 22, 1097
- Komminoth P, 1996, Nonradioactive In Situ Hybridization Application Manual, 126
- 10.1016/0014-4827(86)90520-3 · DOI · Paper page
- 10.1016/S0940-2993(11)80029-1 · doi:10.1016/s0940-2993(11)80029-1
- 10.1136/jcp.45.5.416 · DOI · Paper page
- 10.1177/002215540205001002 · DOI
- 10.1007/BF02899522 · DOI
- Lucas R, 1998, Blood, 92, 4730, 10.1182/blood.V92.10.3977 · doi:10.1182/blood.v92.10.3977
- Miyachi K, 1978, J Immunol, 121, 2228, 10.4049/jimmunol.121.6.2228 · DOI · Paper page
- 10.1016/S0021-9258(18)80079-5 · DOI · Paper page
- 10.1111/j.1699-0463.1999.tb01503.x · doi:10.1111/j.1699-0463.1999.tb01503.x
- 10.1097/00004347-199601000-00004 · doi:10.1097/00004347-199601000-00004
- 10.1046/j.1365-2559.1998.00323.x · doi:10.1046/j.1365-2559.1998.00323.x
- 10.1007/BF01082445 · doi:10.1007/bf01082445
- Sakamoto S, 1999, Oncology Rep, 6, 1353
- 10.1177/030098589503200120 · doi:10.1177/030098589503200120
- 10.1083/jcb.123.3.513 · DOI · Paper page
- 10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9 · DOI · Paper page
- Schwarting R, 1993, Lab Invest, 68, 597
- 10.1002/path.1711650213 · DOI · Paper page
- 10.1016/0092-8674(92)90416-A · DOI · Paper page
- Snedecor GW, 1967, Statistical Methods
- Thomas ME, 2002, Am J Physiol, 283, F640
- 10.1083/jcb.107.5.1623 · DOI · Paper page
- 10.1093/ajcp/110.1.24 · DOI
- 10.1002/path.1711680103 · DOI
- 10.1016/S0003-9969(98)00031-4 · doi:10.1016/s0003-9969(98)00031-4
- 10.1093/carcin/18.4.605 · DOI · Paper page
- 10.1111/j.1365-2559.1991.tb00891.x · doi:10.1111/j.1365-2559.1991.tb00891.x
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Neural stem cell proliferation is decreased in schizophrenia, but not in depression
Reif, A., Fritzen, S., Finger, M., Strobel, A., Lauer, M., Schmitt, A., & Lesch, K.-P. (2006) · Molecular Psychiatry · doi:10.1038/sj.mp.4001791 · Cited by 630
Teekakirikul, P., Eminaga, S., Toka, O., Alcalai, R., Wang, L., Wakimoto, H., Nayor, M., Konno, T., Gorham, J. M., Wolf, C. M., et al. (2010) · Journal of Clinical Investigation · doi:10.1172/jci42028 · Cited by 448
A critical developmental switch defines the kinetics of kidney cyst formation after loss of Pkd1
Piontek, K., Menezes, L. F., Garcia-Gonzalez, M. A., Huso, D. L., & Germino, G. G. (2007) · Nature Medicine · doi:10.1038/nm1675 · Cited by 425
Linville, R. M., DeStefano, J. G., Sklar, M. B., Xu, Z., Farrell, A. M., Bogorad, M. I., Chu, C., Walczak, P., Cheng, L., Mahairaki, V., et al. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2018.10.023 · Cited by 199
Ye, W., Mairet‐Coello, G., Pasoreck, E., & DiCicco‐Bloom, E. (2009) · Developmental Neurobiology · doi:10.1002/dneu.20680 · Cited by 43
Metadata
- Authors
- Muskhelishvili, L., Latendresse, J. R., Kodell, R. L., & Henderson, E. B.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 2003-12
- Volume
- 51
- Issue
- 12
- Pages
- 1681-1688
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2164543039
- Cited by
- 236
- References
- 1
- FWCI
- 2.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- journals.sagepub.com/page/policies/text-and-data-mining-license (tdm)
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- Muskhelishvili, L., Latendresse, J. R., Kodell, R. L., & Henderson, E. B. (2003). Evaluation of Cell Proliferation in Rat Tissues with BrdU, PCNA, Ki-67(MIB-5) Immunohistochemistry and In Situ Hybridization for Histone mRNA. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/002215540305101212
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