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Cited by 122Bacterial biofilms and quorum sensingBurkholderia infections and melioidosisCystic Fibrosis Research Advances

Iron Availability Influences Aggregation, Biofilm, Adhesion and Invasion of Pseudomonas Aeruginosa and Burkholderia Cenocepacia

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Berlutti, F., Morea, C., Battistoni, A., Sarli, S., Cipriani, P., Superti, F., Ammendolia, M.G., Valenti, P.·2005·International Journal of Immunopathology and Pharmacology
DOI 10.1177/039463200501800407
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Abstract

Pseudomonas aeruginosa and Burkholderia cenocepacia are predominant opportunistic pathogens in cystic fibrosis (CF) patients. In healthy humans the lower respiratory tract as well as all mucosa, contains a very low free iron concentration (10(-18) M), while in CF patients sputum iron concentration is very high, showing a median value of 63x10(-6) M. Accumulation of catalytic reactive iron heavily contributes to subsequent clinical complications in the lung disorders by the production of reactive oxygen species and increases bacterial growth and virulence. The data reported in this study indicate that low iron concentration (Fe3+ 1 microM)induced free-living forms and motility both in P. aeruginosa and B. cenocepacia, while high iron concentrations (Fe3+ 10 and 100 microM) stimulated aggregation and biofilm formation already in the fluid phases, so demonstrating that aggregation and biofilm formation are positively iron-modulated in these bacteria. Moreover, the different morphological forms (free-living, aggregates and biofilm) showed different capabilities of adhering and invading the bronchial cell line A549. P. aeruginosa PAO1 aggregates, and mostly biofilm, exerted the highest adhesion efficiency, while B. cenocepacia PV1 aggregates or biofilm the lowest. A significant reduction in invasion efficiency by P. aeruginosa biofilm and a significant increase in cell internalization by B. cenocepacia biofilm has been reported. Therefore, the iron availability is an important signal to which P. aeruginosa and B. cenocepacia counteract by leaving the motile free-living forms and entering into a new lifestyle, i.e. biofilm. These data could contribute to explain that the iron-overload of the sputum of CF patients, inducing nonmotile forms, aggregates and biofilm, may facilitate penetration of host epithelial barriers contributing to the establishment of infection, colonization, persistence and systemic spread of these opportunistic pathogens.

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  • In cystic fibrosis patients, high bronchoalveolar iron shifts the balance between apo- and holo-forms of LF towards the latter, compromising the antibiofilm activity of LF.

    “This shifts the balance between apoand holo-forms of LF towards the latter, thus compromising the antibiofilm activity of LF (Berlutti et al., 2005;Rogan et al., 2004;Stites et al., 1999).”

    From Iron in infection and immunity (2020) · cites this paper as Berlutti et al., 2005

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Authors
Berlutti, F., Morea, C., Battistoni, A., Sarli, S., Cipriani, P., Superti, F., Ammendolia, M. G., & Valenti, P.
Affiliations
  • Sapienza University of Rome (IT)
  • University of Rome Tor Vergata (IT)
  • Istituto Superiore di Sanità (IT)
  • Department of Experimental Medicine II University of Naples; ITALY
Corresponding author
Valenti, P.
ORCID
Venue
International Journal of Immunopathology and Pharmacology
Publisher
SAGE Publications
Published
2005-10
Volume
18
Issue
4
Pages
661-670
ISSN
  • 0394-6320 (ISSN-L)
  • 2058-7384
DOI
10.1177/039463200501800407
OpenAlex
W1591767079
Cited by
122
References
27
Citations by year
2026: 4 · 2025: 8 · 2024: 3 · 2023: 11 · 2022: 5 · 2021: 6 · 2020: 12 · 2019: 6 · 2018: 6 · 2017: 6 · 2016: 7 · 2015: 5 · 2014: 6 · 2013: 4 · 2012: 9
FWCI
1.25
Type
journal article
Language
en
Open access
Licence
Locations
  • International Journal of Immunopathology and Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
  • Cineca Institutional Research Information System (Tor Vergata University) (submittedVersion, cc-by-nc-nd)
  • IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Health Sciences › Medicine › Epidemiology › Burkholderia infections and melioidosis
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
Keywords
iron availability, Pseudomonas aeruginosa, Burkholderia cenocepacia, biofilm formation, bacterial aggregation, bacterial adhesion, host cell invasion, cystic fibrosis, bacterial motility, opportunistic pathogens, A549 cells
MeSH terms
Bronchi, Cell Adhesion, Fluorescent Dyes, Humans, Iron, Microscopy, Electron, Scanning, Pseudomonas aeruginosa, Burkholderia cepacia, Biofilms, Cell Line, Tumor

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