The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
A New Approach to Drug Discovery: High-Throughput Screening of Microbial Natural Extracts against Aspergillus fumigatus Using Resazurin
Open accessAbstract
Natural products are an inexhaustible source for drug discovery. However, the validation and selection of primary screening assays are vital to guarantee a selection of extracts or molecules with relevant pharmacological action and worthy of following up. The assay must be rapid, simple, easy to implement, and produce quick results and preferably at a low cost. In this work, we developed and validated a colorimetric microtiter assay using the resazurin viability dye. The parameters of the resazurin method for high-throughput screening (HTS) using natural extracts against Aspergillus fumigatus were optimized and set up. The extracts plus RPMI-1640 modified medium containing the spores and 0.002% resazurin were added per well. The fluorescence was read after 24 to 30 h of incubation. The resazurin proved to be as suitable as Alamar Blue for determining the minimal inhibitory concentration of different antifungals against A. fumigatus and effective to analyze fungicidal and fungistatic compounds. An HTS of 12 000 microbial extracts was carried out against two A. fumigatus strains, and 2.7% of the extracts displayed antifungal activity. Our group has been the first to use this methodology for screening a collection of natural extracts to identify compounds with antifungal activity against the medically important human pathogen A. fumigatus.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Tetrazolium salts, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 2,3-bis {2-methoxy-4-nitro-5-[(sulfenylamino) carbonyl]-2H-tetrazolium-hydroxide} (XTT), are often used in the MIC endpoint determination for both antifungal and antibacterial microdilution assays [57], [58], [59], [60].”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [59]
Citations
Cites
Zhang, J.-H., Chung, T. D. Y., & Oldenburg, K. R. (1999) · SLAS Discovery · doi:10.1177/108705719900400206 · Cited by 6,847
Aspergillus fumigatus and Aspergillosis
Latgé, J.-P. (1999) · Clinical Microbiology Reviews · doi:10.1128/cmr.12.2.310 · Cited by 2,307
Self-citationda Silva Ferreira, M. E., Kress, M. R. V. Z., Savoldi, M., Goldman, M. H. S., Härtl, A., Heinekamp, T., Brakhage, A. A., & Goldman, G. H. (2006) · Eukaryotic Cell · doi:10.1128/ec.5.1.207-211.2006 · Cited by 403
Self-citationPulmonary aspergillosis: a clinical update
Zmeili, O. S., & Soubani, A. (2007) · QJM · doi:10.1093/qjmed/hcm035 · Cited by 276
Update onin vitrocytotoxicity assays for drug development
Niles, A. L., Moravec, R. A., & Riss, T. (2008) · Expert Opinion on Drug Discovery · doi:10.1517/17460441.3.6.655 · Cited by 157
Larson, E. M., Doughman, D. J., Gregerson, D. S., & Obritsch, W. F. (1997) · PubMed · Cited by 126
Manavathu, E. K. (2004) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkh066 · Cited by 76
Martín‐Mazuelos, E. (2003) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkg384 · Cited by 71
A rapid resazurin bioassay for assessing the toxicity of fungicides
Fai, P. B. A., & Grant, A. (2009) · Chemosphere · doi:10.1016/j.chemosphere.2008.11.078 · Cited by 71
Screening for Antifungal Peptides and Their Modes of Action in Aspergillus nidulans
Mania, D., Hilpert, K., Ruden, S., Fischer, R., & Takeshita, N. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.01560-10 · Cited by 54
Cited by
Sorted by citations
Items 101–144 of 144
Huseynzada, A., Mori, M., Meneghetti, F., Israyilova, A., Güney, E., Sayın, K., Chiarelli, L. R., Demiralp, M., Hasanova, U., & Abbasov, V. М. (2022) · Crystals · doi:10.3390/cryst13010052 · Cited by 4
Cebrián, E., Rodrı́guez, M., Álvarez, M., Delgado, J., & Núñez, F. (2023) · LWT · doi:10.1016/j.lwt.2023.115576 · Cited by 4
Barros, I. L. E., Veiga, F. F., Corrêa, J. L., Jarros, I. C., Negri, M., & Svidzinski, T. I. E. (2021) · Acta Scientiarum Biological Sciences · doi:10.4025/actascibiolsci.v43i1.55700 · Cited by 4
Aspergillus fumigatus can display persistence to the fungicidal drug voriconazolePreprint
Scott, J., Valero, C., Mato-López, Á., Donaldson, I. J., Roldán, A., Chown, H., van Rhijn, N., Lobo-Vega, R., Gago, S., Furukawa, T., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.05.16.491816 · Cited by 4
Self-citationLaskaris, P., Vicentefranqueira, R., Helynck, O., Jouvion, G., Calera, J. A., du Merle, L., Suzenet, F., Buron, F., de Sousa, R. A., Mansuy, D., et al. (2018) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.02510-17 · Cited by 3
Self-citationGuo, F., Li, M., Wang, Y.-X., Fang, H., Qu, T., Zhang, B., Li, S., Xiao, L., & Du, F. (2025) · Pest Management Science · doi:10.1002/ps.70418 · Cited by 3
Naamidine A reveals a promising zinc-binding strategy for topical antifungal therapy
Puumala, E., Nabeela, S., Thornburg, C. C., Grkovic, T., Uppuluri, P., Whitesell, L. J., O’Keefe, B. R., Robbins, N., & Cowen, L. E. (2024) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01194-24 · Cited by 3
Tackling microbial threats in agriculture with integrative imaging and computational approaches
Singh, N. K., Dutta, A., Puccetti, G., & Croll, D. (2020) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2020.12.018 · Cited by 4
Patel, J. A., Patel, N. B., Maisuriya, P. K., Tiwari, M. R., & Purohit, A. C. (2021) · Letters in Organic Chemistry · doi:10.2174/1570178618666210521150011 · Cited by 3
Nogueira-Lima, S. H. C., Gomes, P. W. P., Navegantes-Lima, K. C., Reis, J. D. E., Carvalho, A. R. V., Pamplona, S. D. G. S. R., Muribeca, A. D. J. B., Silva, M., Monteiro, M. C., & Silva, C. Y. Y. E. (2022) · Metabolites · doi:10.3390/metabo12111083 · Cited by 3
Self-citationPatel, N. B., Maisuria, P. N., Gujarati, A. V., & Patel, D. K. (2023) · Current Bioactive Compounds · doi:10.2174/0115734072265828231010050909 · Cited by 3
Miyazawa, K., Umeyama, T., Takatsuka, S., Muraosa, Y., Hoshino, Y., Yano, S., Abe, K., & Miyazaki, Y. (2024) · Medical Mycology · doi:10.1093/mmy/myae011 · Cited by 3
Smith, T. M., Richie, D. L., & Tao, J. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-3673-1_11 · Cited by 3
Fernández-Pastor, I., González-Menéndez, V., Martínez, K. A., Serrano, R., Mackenzie, T. A., Benitez, G. A., Casares-Porcel, M., Genilloud, O., & Reyes, F. (2023) · Journal of Fungi · doi:10.3390/jof9090887 · Cited by 3
Self-citationSerna-Cardona, N., Zamora-Leiva, L., Sánchez-Carvajal, E., Claverías, F. P., Cumsille, A., Pentón, K. A., Vivanco, B., Tietze, A. A., Tessini, C., & Cámara, B. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1463911 · Cited by 2
Highly Concentrated Linear Guanidine Amides from the Marine Sipunculid Phascolosoma granulatum
Jennings, L. K., Kaur, N., Ramos, M. C., Reyes, F., Reddy, M. M., & Thomas, O. (2024) · Journal of Natural Products · doi:10.1021/acs.jnatprod.3c01186 · Cited by 2
Girão, M., Murillo-Alba, J., Martı́n, J., Pérez‐Victoria, I., Leite, R. B., Urbatzka, R., Leão, P. N., Carvalho, M. F., & Reyes, F. (2024) · Marine Drugs · doi:10.3390/md22060268 · Cited by 2
Multivalent inhibition of the Aspergillus fumigatus KDNase
Scalabrini, M., Loquet, D., Rochard, C., Marie, M. B., Assailly, C., Brissonnet, Y., Daligault, F., Saumonneau, A., Lambert, A., Grandjean, C., et al. (2024) · Organic & Biomolecular Chemistry · doi:10.1039/d4ob00601a · Cited by 2
Nagpal, K., Rani, N., Sharma, P., Verma, I., Dubey, A., Handa, U., Kansal, V., & Dhankhar, S. (2024) · Current Traditional Medicine · doi:10.2174/0122150838261497231005115943 · Cited by 2
Hartuis, S., Robert, M., Chouaki, T., Morio, F., Álvarez, C., Pape, P. L., & Lavergne, R. (2025) · Medical Mycology · doi:10.1093/mmy/myaf110 · Cited by 2
Tiwari, M. S., Patil, P. D., & Deshmukh, G. (2020) · Environmental and microbial biotechnology · doi:10.1007/978-981-15-7098-8_8 · Cited by 2
Talas, F., Stapley, J., & McDonald, B. A. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.08.07.606886 · Cited by 2
Escuzarmycins A–D, Potent Biofungicides to Control Septoria tritici Blotch
Fernández-Pastor, I., González-Menéndez, V., González, I., Serrano, R., Mackenzie, T. A., Benitez, G. A., Casares-Porcel, M., Genilloud, O., & Reyes, F. (2024) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.4c01303 · Cited by 1
Self-citationNejatie, A., Proceviat, C., Gros, C., Steves, E., Moore, M. M., Vocadlo, D. J., & Bennet, A. J. (2024) · Glycobiology · doi:10.1093/glycob/cwae094 · Cited by 1
Jennings, L. K., Kaur, N., Ramos, M. C., de la Cruz, M., Reddy, M. M., Reyes, F., & Thomas, O. (2025) · Organic & Biomolecular Chemistry · doi:10.1039/d5ob01206f · Cited by 1
Mhlongo, J. K., Beukes, D. R., & Trindade, M. (2017) · Springer eBooks · doi:10.1007/978-3-319-60408-4_20 · Cited by 1
Incidences of Aspergillus sp infections in Brazil
Gomes, F. J. F., & Motta, R. R. (2023) · Contribuciones a las Ciencias Sociales · doi:10.55905/revconv.16n.12-004 · Cited by 1
Rajakulasooriya, R. S. R., Fernando, S., Gunasekara, C. P., Jayaweera, P. M., Kumarasinghe, K. G. U. R., Thabrew, H., Chan, E., Buddhika, R. B. J., Weerasinghe, G. G. Y. H., & Karunarathna, K. A. A. U. (2025) · BMC Pharmacology and Toxicology · doi:10.1186/s40360-025-00850-1 · Cited by 1
Anooja, V. V., Neelima, S., Athira, P. P., Anju, M., V, Archana, K., Mohan, A. S., Revathy, M. R., Kesavan, D., & Philip, R. (2025) · Microbial Pathogenesis · doi:10.1016/j.micpath.2025.107518 · Cited by 1
Avaliação do uso da resazurina em teste de suscetibilidade in vitro frente a Sporothrix brasiliensis
Roca, B. M., Poester, V. R., Mattei, A. S., Klafke, G. B., Ramis, I. B., & Xavier, M. O. (2019) · VITTALLE - Revista de Ciências da Saúde · doi:10.14295/vittalle.v31i2.8907 · Cited by 1
Caerulines A and B, Flavonol Diacylglycosides from Persea caerulea
Álvarez-Caballero, J. M., Suárez, L. E. C., Coy-Barrera, E. D., Carrasco‐Pancorbo, A., Olmo‐García, L., Martı́n, J., de la Cruz, M., Pérez‐Victoria, I., & Reyes, F. (2021) · ACS Omega · doi:10.1021/acsomega.1c04255 · Cited by 1
Cell-based bioluminescent high-throughput screening methods in antibacterial drug discovery
Nybond, S. (2015) · Työväentutkimus Vuosikirja
Investigating the effect of particle size on the antibacterial activity of gold nanoparticles
Maphasa, R. E. (2017) · University of the Western Cape Electronic Theses and Dissertations Repository (University of the Western Cape)
Avaliação da atividade antimicrobiana de extratos de própolis em diferente maturação
Chada, F. J. G. (2013) · Americanae (AECID Library)
Willaert, R. (2018) · MDPI eBooks · doi:10.3390/books978-3-03897-432-1
Advances in Marine Natural Product Characterisation and Separation Methodologies
Urban, S. (2020) · MDPI eBooks · doi:10.3390/books978-3-03943-129-8
Beattie, S. R., & Krysan, D. J. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-3155-3_3
Patel, J. A., Patel, N. B., Tople, M. S., & Rajani, D. P. (2023) · Indian Journal of Pharmaceutical Sciences · doi:10.36468/pharmaceutical-sciences.1170
A novel pan-fungal screening platform for antifungal drug discovery: proof of principle study
Inman, R., Warris, A., & Bignell, E. (2025) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01328-24 · Cited by 1
Talas, F., Stapley, J., & McDonald, B. A. (2026) · Pest Management Science · doi:10.1002/ps.70483
Correa, K. C. S., Ribeiro, G. H., Bueno, O. C., Colnago, L. A., Malavazi, I., & de Souza, D. H. F. (2026) · Polymers · doi:10.3390/polym18030402
Discovery of tRNA-dependent ergosterol aminoacylation in fungi
Yakobov, N. (2020) · theses.fr (ABES) · doi:10.70675/54f07229z7a9bz4a0fz8263z43817d7f7c3c
dos Reis, T. F., Malavazi, I., & Goldman, G. H. (2026) · Methods in molecular biology · doi:10.1007/978-1-0716-5222-0_16
Assessing Sensitivity to Antifungal Plant Compounds or Agrochemicals
Muralidhar, S. H., Fleißner, A., & van Kan, J. A. L. (2026) · Methods in molecular biology · doi:10.1007/978-1-0716-5551-1_13
Metadata
- Authors
- Monteiro, M. C., de la Cruz, M., Cantizani, J., Moreno, C., Tormo, J. R., Mellado, E., De Lucas, J. R., Asensio, F., Valiante, V., Brakhage, A. A., Latgé, J.-P., Genilloud, O., & Vicente, F.
- Affiliations
- Fundación Medina (ES)
- Instituto de Salud Carlos III (ES)
- Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI) (DE)
- Institut Pasteur (FR)
- Corresponding author
- Monteiro, Maria Cândida
- ORCID
- Venue
- SLAS Discovery
- Publisher
- Elsevier BV
- Published
- 2012-04
- Volume
- 17
- Issue
- 4
- Pages
- 542-549
- ISSN
- 2472-5552 (ISSN-L)
- OpenAlex
- W2012818739
- Cited by
- 144
- References
- 15
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 17 · 2023: 17 · 2022: 11 · 2021: 19 · 2020: 16 · 2019: 6 · 2018: 11 · 2017: 7 · 2016: 8 · 2015: 9 · 2014: 7 · 2013: 6
- FWCI
- 4.88
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- SLAS DISCOVERY (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Universidad de Granada
- Fundación MEDINA
- Junta de Andalucía
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Microbial Natural Products and Biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Keywords
- Aspergillus fumigatus, resazurin, high-throughput screening, antifungal activity, minimum inhibitory concentration, drug discovery
- MeSH terms
- Antifungal Agents, Aspergillus fumigatus, Fungi, Humans, Indicators and Reagents, Microbial Sensitivity Tests, Oxazines, Xanthenes, Complex Mixtures, Drug Discovery, High-Throughput Screening Assays
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review