The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Pyrosequencing analysis of the Oral Microflora of healthy adults
Abstract
A good definition of commensal microflora and an understanding of its relation to health are essential in preventing and combating disease. We hypothesized that the species richness of human oral microflora is underestimated. Saliva and supragingival plaque were sampled from 71 and 98 healthy adults, respectively. Amplicons from the V6 hypervariable region of the small-subunit ribosomal RNA gene were generated by PCR, pooled into saliva and plaque pools, and sequenced by means of the Genome Sequencer 20 system at 454 Life Sciences. Data were evaluated by taxonomic and rarefaction analyses. The 197,600 sequences generated yielded about 29,000 unique sequences, representing 22 taxonomic phyla. Grouping the sequences in operational taxonomic units (6%) yielded 3621 and 6888 species-level phylotypes in saliva and plaque, respectively. This work gives a radically new insight into the diversity of human oral microflora, which, with an estimated number of 19,000 phylotypes, is considerably higher than previously reported.
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“The reduction in pathogenic periodontal bacteria in biofilms by mechanical instrumentation [13] through nonsurgical periodontal treatment has been shown to have limited long-term benefits [14,15].”
From A Cross-Talk between Diet and the Oral Microbiome: Balance of Nutrition on Inflammation and Immune System’s Response during Periodontitis (2022) · cites this paper as [15]
Citations
Cites
Pruesse, E., Quast, C., Knittel, K., Fuchs, B. M., Ludwig, W., Peplies, J., & Glöckner, F. O. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm864 · Cited by 6,682
Microbial diversity in the deep sea and the underexplored “rare biosphere”
Sogin, M. L., Morrison, H. G., Huber, J. A., Welch, D. B. M., Huse, S. M., Neal, P. R., Arrieta, J. M., & Herndl, G. J. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605127103 · Cited by 3,803
Defining the normal bacterial flora of the oral cavity
Aas, J. A., Paster, B. J., Stokes, L. N., Olsen, I., & Dewhirst, F. E. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.11.5721-5732.2005 · Cited by 3,092
Schloss, P. D., & Handelsman, J. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.3.1501-1506.2005 · Cited by 2,529
Review and re-analysis of domain-specific 16S primers
Baker, G. C., Smith, J. J., & Cowan, D. A. (2003) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2003.08.009 · Cited by 1,934
Pyrosequencing enumerates and contrasts soil microbial diversity
Roesch, L. F. W., Fulthorpe, R. R., Riva, A., Casella, G., Hadwin, A. K. M., Kent, A. D., Daroub, S. H., Camargo, F. A. D. O., Farmerie, W. G., & Triplett, E. W. (2007) · The ISME Journal · doi:10.1038/ismej.2007.53 · Cited by 1,891
A Sequencing Method Based on Real-Time Pyrophosphate
Ronaghi, M., Uhlén, M., & Nyrén, P. (1998) · Science · doi:10.1126/science.281.5375.363 · Cited by 1,630
The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis
Cole, J. R. (2004) · Nucleic Acids Research · doi:10.1093/nar/gki038 · Cited by 1,478
Accuracy and quality of massively parallel DNA pyrosequencing
Huse, S. M., Huber, J. A., Morrison, H. G., Sogin, M. L., & Welch, D. B. M. (2007) · Genome biology · doi:10.1186/gb-2007-8-7-r143 · Cited by 1,280
Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults
Aas, J. A., Griffen, A. L., Dardis, S. R., Lee, A. M., Olsen, I., Dewhirst, F. E., Leys, E. J., & Paster, B. J. (2008) · Journal of Clinical Microbiology · doi:10.1128/jcm.01410-07 · Cited by 952 · cited 1×
“Recent studies where cloning and sequencing approaches of microbial 16S rDNA were applied to the oral microflora have identified still more new species (Aas et al., 2008; Preza et al., 2008; Riggio et al., 2008).”
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Occlusal Caries: Biological Approach for Its Diagnosis and Management
Carvalho, J. C., Dige, I., Mačhiulskienė, V., Qvist, V., Bakhshandeh, A., Parolo, C. C. F., & Maltz, M. (2016) · Caries Research · doi:10.1159/000448662 · Cited by 2,159
The oral microbiome in health and disease
Wade, W. G. (2013) · Pharmacological Research · doi:10.1016/j.phrs.2012.11.006 · Cited by 1,347
Environmental and Gut Bacteroidetes: The Food Connection
Thomas, F., Hehemann, J., Rebuffet, E., Czjzek, M., & Michel, G. (2011) · Frontiers in Microbiology · doi:10.3389/fmicb.2011.00093 · Cited by 1,398
“Bacteroidetes have also been found in the normal microbiota of the oral cavity, either in the saliva or dental plaque (Keijser et al., 2008; Nasidze et al., 2009a,b).”
BackgroundDefining the healthy “core microbiome” of oral microbial communities
Zaura, E., Keijser, B. J., Huse, S. M., & Crielaard, W. (2009) · BMC Microbiology · doi:10.1186/1471-2180-9-259 · Cited by 1,323
“Since saliva is easily and noninvasively accessible it is a popular sample in oral epidemiology and microbiome diversity [4,16] studies.”
InfluentialSelf-citationSegata, N., Haake, S. K., Mannon, P., Lemon, K. P., Waldron, L., Gevers, D., Huttenhower, C., & Izard, J. (2012) · Genome Biology · doi:10.1186/gb-2012-13-6-r42 · Cited by 1,076
“The oral microbiome as revealed in this investigation was generally consistent with earlier studies [11,13,14,22,66,67].”
Result
Metadata
- Authors
- Keijser, B. J. F., Zaura, E., Huse, S. M., van der Vossen, J. M. B. M., Schuren, F. H. J., Montijn, R. C., ten Cate, J. M., & Crielaard, W.
- Affiliations
- Marine Biological Laboratory (US)
- TNO Quality of Life, Business Unit Food and Biotechnology Innovations, Microbial Genomics Group, Zeist, The Netherlands;
- Venue
- Journal of Dental Research
- Publisher
- SAGE Publications
- Published
- 2008-11
- Volume
- 87
- Issue
- 11
- Pages
- 1016-1020
- ISSN
- 0022-0345 (ISSN-L)
- 1544-0591
- OpenAlex
- W2103805072
- Cited by
- 669
- References
- 27
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 33 · 2023: 16 · 2022: 39 · 2021: 26 · 2020: 30 · 2019: 24 · 2018: 47 · 2017: 45 · 2016: 41 · 2015: 42 · 2014: 49 · 2013: 50 · 2012: 65
- FWCI
- 53.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Dental Research (publishedVersion)
- PubMed (publishedVersion)
- UvA-DARE (University of Amsterdam) (publishedVersion)
- UvA-DARE (University of Amsterdam) (submittedVersion)
- VU Research Portal (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- oral microbiota, species richness, saliva, supragingival plaque, 16S rRNA gene, operational taxonomic units, phylotypes, microbial diversity, healthy adults
- MeSH terms
- Adult, Dental Plaque, DNA, Bacterial, Humans, RNA, Bacterial, RNA, Ribosomal, 16S, Saliva, Bacterial Typing Techniques, Sequence Analysis, DNA
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