Endometrial gene expression in early pregnancy: Lessons from human ectopic pregnancy
Open accessAbstract
Human endometrium undergoes modifications in preparation for embryonic implantation. This study investigated in vivo the endocrine effects of pregnancy on the endometrium, using the model of ectopic pregnancy. Endometrial biopsies from 9 subjects with ectopic pregnancy (Preg) were compared with 8 and 6 samples of mid and late secretory endometrium, respectively. After hybridizing with Affymetrix HGU133 Plus 2 chips, data were analyzed using GeneSpring GX and Ingenuity Pathways Analysis. From 54,675 genes, 3021 genes were significantly differentiated when mid-secretory endometrium was compared with the Preg (Volcano plot; P < .05, >or=2-fold change).The complement and coagulation cascade, phospholid degradation, glycosphingolipid biosynthesis (globoseries), retinol metabolism, antigen presentation pathway, glycosphingolipid biosynthesis, and O-glycan biosynthesis were main significant canonical pathways found in Preg samples. Validation was done with reverse transcriptase polymerase chain reaction. In conclusion, the ectopic embryo has a significant impact, by an endocrine mechanism, on endometrium, when compared with the window of implantation.
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Overexpression of the RHOJ gene has been shown in ectopic endometrium.
“Overexpression of the RHOJ gene has been shown in ectopic endometrium [191].”
From Endometriosis: Epidemiology, Classification, Pathogenesis, Treatment and Genetics (Review of Literature) (2021) · cites this paper as [191]
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- Authors
- Savaris, R. F., Hamilton, A. E., Lessey, B. A., & Giudice, L. C.
- Affiliations
- Universidade Federal do Rio Grande do Sul (BR)
- University of California, San Francisco (US)
- Prisma Health (US)
- Corresponding author
- Giudice, Linda C.
- Venue
- Reproductive Sciences
- Published
- 2008-10
- Volume
- 15
- Issue
- 8
- Pages
- 797-816
- ISSN
- 1933-7191 (ISSN-L)
- 1933-7205
- PMC
- PMC2882188
- OpenAlex
- W1971435240
- Cited by
- 37
- References
- 69
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 1 · 2013: 2 · 2012: 1
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Reproductive Sciences (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (U54-HD-35041, U54HD055764, U54HD035041)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Ectopic Pregnancy Diagnosis and Management
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Keywords
- endometrial gene expression, ectopic pregnancy, secretory endometrium, early pregnancy, complement and coagulation cascade, phospholipid degradation, glycosphingolipid biosynthesis, vitamin A metabolism, antigen presentation, O-glycan biosynthesis, embryo-endometrium interaction, implantation window
- MeSH terms
- Adult, Endometrium, Female, Humans, Middle Aged, Pregnancy, Pregnancy, Ectopic, RNA, Messenger, Transcription, Genetic, Gene Expression, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Young Adult
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