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Cited by 483

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Differentiation of Tr1 cells by immature dendritic cells requires IL-10 but not CD25+CD4+ Tr cells

Levings, Megan K., Gregori, Silvia, Tresoldi, Eleonora, Cazzaniga, Sabrina, Bonini, Chiara, Roncarolo, Maria Grazia·2005·Blood
DOI 10.1182/blood-2004-03-1211
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Abstract

Dendritic cells (DCs) are specialized antigen-presenting cells that monitor the antigenic environment and activate naive T cells. The role of DCs is not only to sense danger but also to tolerize the immune system to antigens encountered in the absence of maturation/inflammatory stimuli. Indeed, if a naive T cell encounters its antigen on immature DCs (iDCs), it may differentiate into a T-regulatory (Tr) rather than a T-effector cell. However, little is known about the mechanisms by which iDCs differentiate Tr cells. We developed a standardized and highly reproducible protocol to differentiate Tr cells by repetitive exposure of naive peripheral blood CD4+ T cells to allogeneic iDCs. The resultant Tr cells are phenotypically and functionally identical to type 1 Tr (Tr1) cells because their generation requires production of IL-10 by iDCs, and they suppress T-cell responses through an interleukin-10 (IL-10)– and a transforming growth factor β (TGF-β)–dependent mechanism. In addition, Tr1 cells induced by iDCs do not require the presence of CD4+CD25+ Tr cells for their generation, nor do they express high constitutive levels of CD25 or the transcription factor FoxP3. Thus, iDCs can drive the differentiation of Tr1 cells and can be used to generate large numbers of alloantigen-specific Tr1 cells for clinical use as a cellular therapy to restore peripheral tolerance.

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Metadata

Authors
Levings, M. K., Gregori, S., Tresoldi, E., Cazzaniga, S., Bonini, C., & Roncarolo, M. G.
Venue
Blood
Publisher
American Society of Hematology
Published
2005-02-01
Volume
105
Issue
3
Pages
1162-1169
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
DOI
10.1182/blood-2004-03-1211
OpenAlex
W2082992807
Cited by
483
References
2
FWCI
12.68
Type
journal article
Language
en
Open access
closed

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