CoChat
Sign Up
Cited by 118Immunotherapy and Immune ResponsesT-cell and B-cell ImmunologyCancer Immunotherapy and Biomarkers

Heavy chain ferritin activates regulatory T cells by induction of changes in dendritic cells

Gray, Christian P., Arosio, Paolo, Hersey, Peter·2002·Blood
DOI 10.1182/blood.v99.9.3326
Open this paper in CoChat — free account

Abstract

Heavy chain ferritin (H-ferritin) is a component of the iron-binding protein, ferritin. We have previously shown that H-ferritin inhibits anti-CD3-stimulated lymphocyte proliferation and that this was due to increased production of interleukin-10 (IL-10). In the present study we have shown that induction of IL-10 production was due to effects of H-ferritin on adherent antigen-presenting cells (APCs) in blood and monocyte-derived dendritic cells (MoDCs). IL-10 was produced by a subpopulation of CD4 T cells, which expressed the CD25 component of the IL-2 receptor and the CTLA-4 receptor characteristic of regulatory T cells. The changes induced in MoDCs were compared with those induced by CD40L and their significance tested by inhibition with monoclonal antibodies. These studies indicated that H-ferritin induced relatively greater expression of CD86 and B7-H1 on MoDCs and that monoclonal antibodies against their receptors, CTLA-4 and programmed death receptor-1 (PD-1), inhibited IL-10 production from the regulatory T cells. H-ferritin did not appear to induce direct production of the cytokines IL-2, IL-4, IL-6, IL-10, IL-12, or interferon-gamma from the DCs. These results are consistent with the thesis that H-ferritin induces B7-H1 and CD86 (B7-2) on APCs, which in turn induce IL-10 production from regulatory T cells. This is possibly one mechanism by which melanoma cells may induce changes in APCs in the vicinity of the tumor and result in suppression of immune responses by induction of regulatory T cells.

Integrity signals

Registry

Crossref · 10.1182/blood.v99.9.3326

Cites

60 of 60 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • FTH is secreted by melanoma cells and circulates in plasma.

    “FTH is secreted by melanoma cells and circulates in the plasma.”

    From Iron in infection and immunity (2020) · cites this paper as Gray et al., 2002

  • FTH acts on antigen-presenting cells and induces expression of CD86 and B7-H1.

    “FTH then acts on antigen-presenting cells (APC) and induces the expression of CD86 and B7-H1 (Gray et al., 2002(Gray et al., , 2003)).”

    From Iron in infection and immunity (2020) · cites this paper as Gray et al., 2002

  • Iron present in FTH affects the function of Tregs, as demonstrated in malignant melanoma patients.

    “Indirectly, iron present in FTH affects the function of T regs as demonstrated in malignant melanoma patients.”

    From Iron in infection and immunity (2020) · cites this paper as Gray et al., 2002

  • Iron binding and release by Tf are coordinated by a conformational change in which the two subdomains of each lobe open, and the N1, N2, C1, and C2 domains twist.

    “Iron binding and release are coordinated by a conformational change in which the two subdomains of each lobe open, and the N1, N2, C1, and C2 domains twist (114, 115).”

    From Intracellular iron transport and storage: from molecular mechanisms to health implications (2008) · cites this paper as [114]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Gray, C. P., Arosio, P., & Hersey, P.
Affiliations
University of Brescia (IT)
ORCID
Venue
Blood
Publisher
American Society of Hematology
Published
2002-05-01
Volume
99
Issue
9
Pages
3326-3334
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
DOI
10.1182/blood.v99.9.3326
OpenAlex
W2012723575
Cited by
118
References
60
Citations by year
2026: 2 · 2025: 4 · 2024: 1 · 2023: 7 · 2022: 2 · 2021: 2 · 2020: 10 · 2019: 6 · 2018: 5 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 2 · 2012: 5
FWCI
2.41
Type
journal article
Language
en
Open access
closed
Locations
  • Blood (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Research Information System (Università degli Studi di Brescia) (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Health Sciences › Medicine › Oncology › Cancer Immunotherapy and Biomarkers
Keywords
heavy chain ferritin, regulatory T cells, dendritic cells, monocyte-derived dendritic cells, IL-10, antigen-presenting cells, CD86, PD-L1, CTLA-4, PD-1, CD25, CD40 ligand, melanoma, immunosuppression, iron-binding proteins
MeSH terms
Cell Communication, Cell Differentiation, Dendritic Cells, Ferritins, Humans, Lymphocyte Activation, T-Lymphocytes, Receptors, Interleukin-2, CD4-Positive T-Lymphocytes, Cytokines, Interleukin-10, CD40 Ligand

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review