The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
The earliest subpopulation of mouse thymocytes contains potent T, significant macrophage, and natural killer cell but no B-lymphocyte potential
Abstract
The B-lymphocyte potential of progenitor thymocytes and whether the thymus is colonized by common lymphocyte progenitor cells have been subjects of considerable debate. Herein, we have used limiting dilution analysis to determine the lineage potential of phenotypically defined subpopulations of CD4–CD8– double-negative thymocytes. Culture systems used showed single-hit kinetics and had a high plating efficiency for B-, myeloid, and natural killer cell development. The T-cell potential of sorted cells was confirmed by transferring cells to fetal thymus organ cultures. Our results indicate that the earliest population of CD117+ double-negative cells, although containing potent T-cell developmental potential and significant myeloid and natural killer potential, does not have any residual B-cell potential. Gene transcription analysis also indicated that these double-negative cells contained abundant T and myeloid, but not B cell–specific transcripts. The implications of these results within the context of current models of thymocyte development are discussed.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Whereas ETP and DN2 cells can generate myeloid cells if removed from the thymus, pro-T cells that have differentiated past the stage of PU.1 expression in the thymus (i.e., from the DN2 stage to the DN3 stage or later) do not make myeloid cells under these conditions”
From Mechanisms of Action of Hematopoietic Transcription Factor PU.1 in Initiation of T-Cell Development (2019) · cites this paper as [54]
Citations
Cites
Identification of Clonogenic Common Lymphoid Progenitors in Mouse Bone Marrow
Kondo, M., Weissman, I. L., & Akashi, K. (1997) · Cell · doi:10.1016/s0092-8674(00)80453-5 · Cited by 2,253
IMMUNOLOGICAL FUNCTION OF THE THYMUS
MILLER, J. (1961) · The Lancet · doi:10.1016/s0140-6736(61)90693-6 · Cited by 1,396
Deficient T Cell Fate Specification in Mice with an Induced Inactivation of Notch1
Radtke, F., Wilson, A., Stark, G., Bauer, M., van Meerwijk, J. P. M., MacDonald, H. R., & Aguet, M. (1999) · Immunity · doi:10.1016/s1074-7613(00)80054-0 · Cited by 1,393
Induction of T Cell Development from Hematopoietic Progenitor Cells by Delta-like-1 In Vitro
Schmitt, T. M., & Zúñiga-Pflücker, J. C. (2002) · Immunity · doi:10.1016/s1074-7613(02)00474-0 · Cited by 1,120
Karasuyama, H., & Melchers, F. (1988) · European Journal of Immunology · doi:10.1002/eji.1830180115 · Cited by 1,090
The ikaros gene is required for the development of all lymphoid lineages
Georgopoulos, K., Bigby, M., Wang, J.-H., Molnar, A., Wu, P., Winandy, S., & Sharpe, A. (1994) · Cell · doi:10.1016/0092-8674(94)90407-3 · Cited by 985
Notch1 Expression in Early Lymphopoiesis Influences B versus T Lineage Determination
Pui, J. C., Allman, D., Xu, L., DeRocco, S. E., Karnell, F. G., Bakkour, S., Lee, J. Y., Kadesch, T. R., Hardy, R. R., Aster, J. C., et al. (1999) · Immunity · doi:10.1016/s1074-7613(00)80105-3 · Cited by 943
Generation of Lymphohematopoietic Cells from Embryonic Stem Cells in Culture
Nakano, T., Kodama, H., & Honjo, T. (1994) · Science · doi:10.1126/science.8066449 · Cited by 866
Multilineage gene expression precedes commitment in the hemopoietic system.Preprint
Hu, M., Krause, D. S., Greaves, M. F., Sharkis, S. J., Dexter, M., Heyworth, C. M., & Enver, T. (1997) · Genes & Development · doi:10.1101/gad.11.6.774 · Cited by 719
Ardavı́n, C., Wu, L., Li, C., & Shortman, K. (1993) · Nature · doi:10.1038/362761a0 · Cited by 657
Cited by
Sorted by citations
Items 101–158 of 158
Speed and navigation control of thymocyte development by the fetal T‐cell gene regulatory network
MacNabb, B. W., & Rothenberg, E. V. (2023) · Immunological Reviews · doi:10.1111/imr.13190 · Cited by 16
Holland, A. M., Zakrzewski, J. L., Goldberg, G. L., Ghosh, A., & van den Brink, M. R. M. (2008) · Seminars in Immunopathology · doi:10.1007/s00281-008-0138-z · Cited by 16
Regulatory factors for initial T lymphocyte lineage specification
Rothenberg, E. V. (2007) · Current Opinion in Hematology · doi:10.1097/moh.0b013e3281de72a8 · Cited by 16
Genetic and Epigenetic Control of Early Lymphocyte Development
Ikawa, T. (2014) · Current topics in microbiology and immunology · doi:10.1007/82_2014_370 · Cited by 15
The transcription factor Duxbl mediates elimination of pre-T cells that fail β-selection
Klein, F., Mitrovic, M., Roux, J., Engdahl, C., von Muenchow, L., Servera, L. A., Fehling, H. J., Pelczar, P., Rolink, A. G., & Tsapogas, P. (2019) · The Journal of Experimental Medicine · doi:10.1084/jem.20181444 · Cited by 15
Self-citationE2a/Pbx1 oncogene inhibits terminal differentiation but not myeloid potential of pro-T cells
Bourette, R. P., Grasset, M.-F., & Mouchiroud, G. (2006) · Oncogene · doi:10.1038/sj.onc.1209777 · Cited by 15
Immunophenotyping parameters as prognostic factors in T-acute leukemia patients
Babušíková, O., Stevulová, L., & FAJTOVA, M. (2009) · Neoplasma · doi:10.4149/neo_2009_06_508 · Cited by 14
Gautreau‐Rolland, L., Boudil, A., Pasqualetto, V., Skhiri, L., Grandin, L., Monteiro, M., Jaïs, J., & Ezine, S. (2010) · The Journal of Immunology · doi:10.4049/jimmunol.0902184 · Cited by 14
Klein, F., von Muenchow, L., Capoferri, G., Heiler, S., Servera, L. A., Rolink, H., Engdahl, C., Rolink, M., Mitrovic, M., Cvijetic, G., et al. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02258 · Cited by 14
Self-citationSmeets, M. F. M. A., Wiest, D. L., & Izon, D. J. (2014) · European Journal of Immunology · doi:10.1002/eji.201444442 · Cited by 13
Rothenberg, E. V., Champhekar, A. S., Damle, S. S., Real, M. M. D., Kueh, H. Y., Li, L., & Yui, M. A. (2013) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.2013.78.020271 · Cited by 13
Oncogenes and the Origins of Leukemias
Brown, G. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23042293 · Cited by 15
Is lineage decision-making restricted during tumoral reprograming of haematopoietic stem cells?
Brown, G., & Sánchez-Garcı́a, I. (2015) · Oncotarget · doi:10.18632/oncotarget.6145 · Cited by 12
The Route of Early T Cell Development: Crosstalk between Epigenetic and Transcription Factors
Chiara, V. D., Daxinger, L., & Staal, F. J. T. (2021) · Cells · doi:10.3390/cells10051074 · Cited by 11
Logical modeling of cell fate specification—Application to T cell commitment
Cacace, E., Collombet, S., & Thieffry, D. (2020) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2020.02.008 · Cited by 11
Hu, Z., Gu, W., Yang, W., Liu, G., Wu, S., & Liu, T. (2019) · Scientific Reports · doi:10.1038/s41598-018-37811-0 · Cited by 8
Kundu, S., Park, E. S., Chung, Y. J., Walker, R. L., Zhu, Y. J., Negi, V., Meltzer, P. S., & Aplan, P. D. (2019) · Scientific Reports · doi:10.1038/s41598-019-53610-7 · Cited by 8
Introduction and Classification of Leukemias
Brown, G. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-0810-4_1 · Cited by 9
TCR‐β chains derived from peripheral γδ T cells can take part in αβ T‐cell development
Bosco, N., Engdahl, C., Bénard, A., Rolink, J., Ceredig, R. H., & Rolink, A. G. (2008) · European Journal of Immunology · doi:10.1002/eji.200838668 · Cited by 8
Self-citationThe versatility of haematopoietic stem cells: implications for leukaemia
Brown, G., Hughes, P., Michell, R. H., & Ceredig, R. (2010) · Critical Reviews in Clinical Laboratory Sciences · doi:10.3109/10408363.2010.530150 · Cited by 7
Self-citationThe Social Norm of Hematopoietic Stem Cells and Dysregulation in Leukemia
Brown, G. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23095063 · Cited by 6
Brown, G., Hughes, P., Ceredig, R., & Michell, R. H. (2011) · Leukemia Research · doi:10.1016/j.leukres.2011.10.010 · Cited by 6
Self-citationLineage Decision-Making within Normal Haematopoietic and Leukemic Stem Cells
Brown, G., Sánchez, L., & Sánchez-Garcı́a, I. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21062247 · Cited by 5
Hematopoietic Stem Cells: Nature and Niche Nurture
Brown, G. (2021) · Bioengineering · doi:10.3390/bioengineering8050067 · Cited by 4
The versatile landscape of haematopoiesis: Are leukaemia stem cells as versatile?
Brown, G., Hughes, P., & Ceredig, R. H. (2012) · Critical Reviews in Clinical Laboratory Sciences · doi:10.3109/10408363.2012.742487 · Cited by 3
Self-citationAre Leukaemic Stem Cells Restricted to a Single Cell Lineage?
Brown, G., Sánchez, L., & Sánchez-Garcı́a, I. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms21010045 · Cited by 3
Proliferation and lineage potential in fetal thymic epithelial progenitor cells
Cook, A. (2010) · ERA · Cited by 3
Problems defining DN2 thymocytes
Ceredig, R., Bosco, N., & Rolink, T. (2008) · Immunology and Cell Biology · doi:10.1038/icb.2008.44 · Cited by 3
Self-citationCell Lineage Affiliation During Hematopoiesis
Brown, G. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26073346 · Cited by 2
Shimba, A., Tani‐ichi, S., Masuda, K., Cui, G., Munakata, S., Abe, S., Kitano, S., Miyachi, H., Kawamoto, H., & Ikuta, K. (2024) · The Journal of Immunology · doi:10.4049/jimmunol.2300483 · Cited by 2
Co-option of lineage plasticity as a hallmark of multipotent acute leukemias
Gutierrez, A., & Kentsis, A. (2026) · Blood Neoplasia · doi:10.1016/j.bneo.2026.100231 · Cited by 1
Eibel, H., Winkler, T., & Ceredig, R. H. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02915 · Cited by 1
Self-citationRole of regulatory T cells in immune tolerance
Swee, L. K. (2009) · edoc (University of Basel) · doi:10.5451/unibas-004811234
Molecular Regulation of Early T-Cell Development in the Thymus
Weerkamp, F. (2005) · RePub (Erasmus University Rotterdam)
Immune regulation by selective estrogen receptor modulators
Bernardi, A. I. (2015) · Gothenburg University Publications Electronic Archive (Gothenburg University)
Mechanisms of Notch signaling specificity in lymphocytes and their leukemic counterparts
Gentek, R. (2013) · UvA-DARE (University of Amsterdam)
Mécanisme de leucémogénèse par les oncogènes SCL et LMO1
Tremblay, M. R. (2009) · doi:10.71781/27559
Role of E-proteins in B Lymphocyte Commitment and Thymocyte Selection
Jones, M. E. (2009) · DukeSpace (Duke University)
Study of lymphocyte development from embryonic stem cells in vitro
Tabatabaei-Zavareh, N. (2011) · cIRcle (University of British Columbia) · doi:10.14288/1.0100552
Transcriptional Regulation of T Cell Lineage Commitment
Rothenberg, E. V. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-374279-7.04006-6
Control of Early T Cell Development by Notch and T Cell Receptor Signals
Fahl, S. P., Coffey, F. J., MacCormack, D., & Wiest, D. L. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-374279-7.04010-8
Generation and Regeneration of T Cells
Kawamoto, H., Ikawa, T., Maeda, T., & Masuda, K. (2016) · Springer eBooks · doi:10.1007/978-4-431-56027-2_2
MYB IS Required for B-Selection During Thymopoises
Duley, A. K. (2009) · doi:10.18130/v3x28d
de Groot, C. J. M., Wagemaker, G., van Dongen, J. J. M., Staal, F. J. T., Weerkamp, F., Baert, M. R. M., Brugman, M. H., Dik, W. A., de Haas, E. F., Visser, T. P., et al. (2005)
Potencial leucemogênico do IL-7R mutante in vivo
Euzébio, M. F. (2019) · doi:10.47749/t/unicamp.2019.1254805
Transcriptional Regulation of T-Cell Lineage Commitment
Rothenberg, E. V. (2024) · Elsevier eBooks · doi:10.1016/b978-0-128-24465-4.00018-1
Détection des anomalies génétiques dans les LAL-T : de la biologie à la clinique
Abdelali, R. B. (2011) · theses.fr (ABES) · doi:10.70675/90f2a16fz3cdez41f5z8dfez0848b91eb777
Upregulation of CD90 correlates with T lineage commitment at the DN2a stage of T cell development
Parikh, D., Gu, K., Tomei, S., Weber, T. S., Naik, S. H., & Chong, M. M. W. (2026) · Frontiers in Immunology · doi:10.3389/fimmu.2026.1940310
Mechanisms of NOTCH1 Mediated Leukemogenesis: A Dissertation
Cullion, K. (2009) · eScholarship@UMassChan · doi:10.13028/xy9r-3310
Metadata
- Authors
- Balciunaite, G., Ceredig, R., & Rolink, A. G.
- Affiliations
- From the Developmental and Molecular Immunology, Department of Clinical and Biological Sciences (DKBW), University of Basel, Basel, Switzerland; and U548 INSERM, DRDC, CEA-G, Grenoble, France.
- Inserm (FR)
- ORCID
- Ceredig, Rhodri
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2005-03-01
- Volume
- 105
- Issue
- 5
- Pages
- 1930-1936
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2062319579
- Cited by
- 158
- References
- 55
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 7 · 2020: 3 · 2019: 9 · 2018: 6 · 2017: 2 · 2016: 6 · 2015: 4 · 2014: 5 · 2013: 6 · 2012: 3
- FWCI
- 5.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- T cell development, double-negative thymocytes, CD4+CD8+ thymocytes, macrophage development, natural killer cell development, CD117, fetal thymus organ culture, gene transcription, single-hit kinetics
- MeSH terms
- Animals, B-Lymphocytes, Cells, Cultured, Hematopoietic Stem Cells, Immune System, Killer Cells, Natural, Kinetics, Macrophages, T-Lymphocytes, Thymus Gland, Immunophenotyping, Cell Lineage, Leukopoiesis, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
