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Cited by 721

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-α-activated vascular endothelium under flow

Open access
Yang, Lin, Froio, Richard M., Sciuto, Tracey E., Dvorak, Ann M., Alon, Ronen, Luscinskas, Francis W.·2005·Blood
DOI 10.1182/blood-2004-12-4942
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Abstract

In vivo, leukocyte transendothelial migration (TEM) occurs at endothelial cell junctions (paracellular) and nonjunctional (transcellular) locations, whereas in vitro models report that TEM is mostly paracellular. The mechanisms that control the route of leukocyte TEM remain unknown. Here we tested the hypothesis that elevated intercellular adhesion molecule-1 (ICAM-1) expression regulates the location of polymorphonuclear leukocyte (PMN) TEM. We used an in vitro flow model of tumor necrosis factor-α (TNF-α)-activated human umbilical vein endothelium cells (HUVECs) or an HUVEC cell line transfected with ICAM-1GFP (green fluorescent protein) and live-cell fluorescence microscopy to quantify the location of PMN adhesion and TEM. We observed robust transcellular TEM with TNF-α-activated HUVECs and ICAM-1GFP immortalized HUVECS (iHUVECs). In contrast, primary CD3+ T lymphocytes exclusively used a paracellular route. Endothelial ICAM-1 was identified as essential for both paracellular and transcellular PMN transmigration, and interfering with ICAM-1 cytoplasmic tail function preferentially reduced transcellular TEM. We also found that ICAM-1 surface density and distribution as well as endothelial cell shape contributed to transcellular TEM. In summary, ICAM-1 promotes junctional and nonjunctional TEM across inflamed vascular endothelium via distinct cytoplasmic tail associations. (Blood. 2005;106:584-592)

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Metadata

Authors
Yang, L., Froio, R. M., Sciuto, T. E., Dvorak, A. M., Alon, R., & Luscinskas, F. W.
Venue
Blood
Publisher
American Society of Hematology
Published
2005-07-15
Volume
106
Issue
2
Pages
584-592
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
DOI
10.1182/blood-2004-12-4942
OpenAlex
W2112682556
Cited by
721
References
42
FWCI
15.37
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-α-activated vascular endothelium under flow. https://app.cochat.ai/papers/10.1182/blood-2004-12-4942
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Yang, L., Froio, R. M., Sciuto, T. E., Dvorak, A. M., Alon, R., & Luscinskas, F. W. (2005). ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-α-activated vascular endothelium under flow. Blood. https://doi.org/10.1182/blood-2004-12-4942

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