The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles
Open accessAbstract
Platelet microparticles are a normal constituent of circulating blood. Several studies have demonstrated positive correlations between thrombotic states and platelet microparticle levels. Yet little is known about the processes by which platelet microparticles are generated in vivo. We now characterize microparticles derived directly from megakaryocytes. Video microscopy of live mouse megakaryocytes demonstrated that microparticles form as submicron beads along the lengths of slender, unbranched micropodia. These microparticles are CD41+, CD42b+, and express surface phosphatidylserine. Megakaryocyte microparticle generation is resistant to inhibition of microtubule assembly, which is critical to platelet formation, and augmented by inhibition of actin polymerization. To determine whether circulating microparticles are derived primarily from activated platelets or megakaryocytes, we identified markers that distinguish between these 2 populations. CD62P and LAMP-1 were found only on mouse microparticles from activated platelets. In contrast, full-length filamin A was found in megakaryocyte-derived microparticles, but not microparticles from activated platelets. Circulating microparticles isolated from mice were CD62P−, LAMP-1− and expressed full-length filamin A, indicating a megakaryocytic origin. Similarly, circulating microparticles isolated from healthy volunteers were CD62P− and expressed full-length filamin A. Cultured human megakaryocytes elaborated microparticles that were CD41+, CD42b+, and express surface phosphatidylserine. These results indicate that direct production by megakaryocytes represents a physiologic means to generate circulating platelet microparticles.
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Self-citationThe Bone Marrow Vascular Niche: Home of HSC Differentiation and Mobilization
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Biological properties of extracellular vesicles and their physiological functions
Yáñez‐Mó, M., Siljander, P. R. ‐., Andreu, Z., Bedina Zavec, A., Borràs, F. E., Buzas, E. I., Buzas, K., Casal, E., Cappello, F., Carvalho, J., et al. (2015) · Journal of Extracellular Vesicles · doi:10.3402/jev.v4.27066 · Cited by 5,926
Shedding microvesicles: artefacts no more
Cocucci, E., Racchetti, G., & Meldolesi, J. (2009) · Trends in Cell Biology · doi:10.1016/j.tcb.2008.11.003 · Cited by 1,742
Turturici, G., Tinnirello, R., Sconzo, G., & Geraci, F. (2014) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00228.2013 · Cited by 483
Isolation and characterization of platelet-derived extracellular vesicles
Aatonen, M. T., Öhman, T., Nyman, T. A., Laitinen, S., Grönholm, M., & Siljander, P. R. ‐. (2014) · Journal of Extracellular Vesicles · doi:10.3402/jev.v3.24692 · Cited by 333
Metadata
- Authors
- Flaumenhaft, R., Dilks, J. R., Richardson, J., Alden, E., Patel-Hett, S. R., Battinelli, E., Klement, G. L., Sola-Visner, M., & Italiano, J. E.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2009-01-29
- Volume
- 113
- Issue
- 5
- Pages
- 1112-1121
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2015445251
- Cited by
- 317
- References
- 48
- FWCI
- 6.68
- Type
- journal article
- Language
- en
- Open access
- green
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- Flaumenhaft, R., Dilks, J. R., Richardson, J., Alden, E., Patel-Hett, S. R., Battinelli, E., Klement, G. L., Sola-Visner, M., & Italiano, J. E. (2009). Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles. Blood. https://doi.org/10.1182/blood-2008-06-163832
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