The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Macrophage-specific gene functions in Spi1-directed innate immunity
Abstract
The Spi1/Pu.1 transcription factor plays a crucial role in myeloid cell development in vertebrates. Despite extensive studies of Spi1, the controlled gene group remains largely unknown. To identify genes dependent on Spi1, we used a microarray strategy using a knockdown approach in zebrafish embryos combined with fluorescence-activated cell sorting of myeloid cells from transgenic embryos. This approach of using knockdowns with specific green fluorescent protein-marked cell types was highly successful in identifying macrophage-specific genes in Spi1-directed innate immunity. We found a gene group down-regulated on spi1 knockdown, which is also enriched in fluorescence-activated cell-sorted embryonic myeloid cells of a spi1:GFP transgenic line. This gene group, representing putative myeloid-specific Spi1 target genes, contained all 5 previously identified Spi1-dependent zebrafish genes as well as a large set of novel immune-related genes. Colocalization studies with neutrophil and macrophage markers revealed that genes cxcr3.2, mpeg1, ptpn6, and mfap4 were expressed specifically in early embryonic macrophages. In a functional approach, we demonstrated that gene cxcr3.2, coding for chemokine receptor 3.2, is involved in macrophage migration to the site of bacterial infection. Therefore, based on our combined transcriptome analyses, we discovered novel early macrophage-specific marker genes, including a signal transducer pivotal for macrophage migration in the innate immune response.
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Citations
Cites
Regulation of B lymphocyte and macrophage development by graded expression of PU.1
DeKoter, R. P., & Singh, H. (2000) · Science · doi:10.1126/science.288.5470.1439 · Cited by 749
Negative cross-talk between hematopoietic regulators: GATA proteins repress PU.1
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The ‘definitive’ (and ‘primitive’) guide to zebrafish hematopoiesis
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PU.1 inhibits GATA-1 function and erythroid differentiation by blocking GATA-1 DNA binding
Zhang, P., Zhang, X., Iwama, A., Yu, C., Smith, K. A., Mueller, B. U., Narravula, S., Torbett, B. E., Orkin, S. H., & Tenen, D. G. (2000) · Blood · doi:10.1182/blood.v96.8.2641 · Cited by 368
Essential role of Jun family transcription factors in PU.1 knockdown–induced leukemic stem cells
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Potential Autoregulation of Transcription Factor PU.1 by an Upstream Regulatory Element
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A novel modulator domain of Ets transcription factors.
Wasylyk, C., Kerckaert, J. P., & Wasylyk, B. (1992) · Genes & Development · doi:10.1101/gad.6.6.965 · Cited by 140
PU.1 Activates Transcription of SHP-1 Gene in Hematopoietic Cells
Wlodarski, P., Zhang, Q., Liu, X., Kasprzycka, M., Marzec, M., & Wasik, M. A. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m607526200 · Cited by 29
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Macrophages: An Inflammatory Link Between Angiogenesis and Lymphangiogenesis
Corliss, B. A., Azimi, M. S., Munson, J. M., Peirce, S. M., & Murfee, W. L. (2016) · Microcirculation · doi:10.1111/micc.12259 · Cited by 356
Holland, C. H., Tanevski, J., Perales-Patón, J., Gleixner, J., Kumar, M. P., Mereu, E., Joughin, B. A., Stegle, O., Lauffenburger, D. A., Heyn, H., et al. (2020) · Genome Biology · doi:10.1186/s13059-020-1949-z · Cited by 323
Buchrieser, J., James, W., & Moore, M. D. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2016.12.020 · Cited by 215
A comparative atlas of single-cell chromatin accessibility in the human brain
Li, Y. E., Preissl, S., Miller, M., Johnson, N. D., Wang, Z., Jiao, H., Zhu, C., Wang, Z., Xie, Y., Poirion, O., et al. (2023) · Science · doi:10.1126/science.adf7044 · Cited by 173
Single-cell multiomics analysis reveals regulatory programs in clear cell renal cell carcinoma
Long, Z., Sun, C., Tang, M., Wang, Y., Ma, J., Yu, J., Wei, J., Ma, J., Wang, B., Xie, Q., et al. (2022) · Cell Discovery · doi:10.1038/s41421-022-00415-0 · Cited by 109
Metadata
- Authors
- Zakrzewska, A., Cui, C., Stockhammer, O. W., Benard, E. L., Spaink, H. P., & Meijer, A. H.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2010-07-22
- Volume
- 116
- Issue
- 3
- Pages
- e1-e11
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1980353886
- Cited by
- 199
- References
- 9
- FWCI
- 4.18
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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