CD146 expression on primary nonhematopoietic bone marrow stem cells is correlated with in situ localization
Open accessAbstract
Nonhematopoietic bone marrow mesenchymal stem cells (BM-MSCs) are of central importance for bone marrow stroma and the hematopoietic environment. However, the exact phenotype and anatomical distribution of specified MSC populations in the marrow are unknown. We characterized the phenotype of primary human BM-MSCs and found that all assayable colony-forming units-fibroblast (CFU-Fs) were highly and exclusively enriched not only in the lin−/CD271+/CD45−/CD146+ stem-cell fraction, but also in lin−/CD271+/CD45−/CD146−/low cells. Both populations, regardless of CD146 expression, shared a similar phenotype and genotype, gave rise to typical cultured stromal cells, and formed bone and hematopoietic stroma in vivo. Interestingly, CD146 was up-regulated in normoxia and down-regulated in hypoxia. This was correlated with in situ localization differences, with CD146 coexpressing reticular cells located in perivascular regions, whereas bone-lining MSCs expressed CD271 alone. In both regions, CD34+ hematopoietic stem/progenitor cells were located in close proximity to MSCs. These novel findings show that the expression of CD146 differentiates between perivascular versus endosteal localization of non-hematopoietic BM-MSC populations, which may be useful for the study of the hematopoietic environment.
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CD146 expression in MSCs plays a key role in the perivascular niche, skeletogenesis, and hematopoietic support in vivo.
“CD146 expression in MSCs plays a key role in the perivascular niche, skeletogenesis, and hematopoietic support in vivo [26,27,28].”
From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [26]
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- Authors
- Tormin, A., Li, O., Brune, J. C., Walsh, S., Schütz, B., Ehinger, M., Ditzel, N., Kassem, M., & Scheding, S.
- Affiliations
- Lund University (SE)
- Skåne University Hospital (SE)
- University of Southern Denmark (DK)
- Corresponding author
- Li, Ou
- Brune, Jan Claas
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2011-05-12
- Volume
- 117
- Issue
- 19
- Pages
- 5067-5077
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- PMC
- PMC3109533
- OpenAlex
- W2118939519
- Cited by
- 460
- References
- 1
- Citations by year
- 2026: 6 · 2025: 13 · 2024: 24 · 2023: 32 · 2022: 17 · 2021: 37 · 2020: 51 · 2019: 34 · 2018: 27 · 2017: 28 · 2016: 40 · 2015: 36 · 2014: 49 · 2013: 30 · 2012: 27
- FWCI
- 18.67
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Lund University Publications (Lund University) (submittedVersion, other-oa)
- University of Southern Denmark Research Portal (University of Southern Denmark) (submittedVersion)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Health Sciences › Medicine › Hematology › Multiple Myeloma Research and Treatments
- Keywords
- CD146, bone marrow mesenchymal stem cells, p75 neurotrophin receptor, perivascular localization, bone marrow stroma, hematopoietic microenvironment, hypoxia, normoxia, hematopoietic stem and progenitor cells, CFU-F, bone lining cells, reticular cells
- MeSH terms
- Animals, Bone Marrow Cells, Cell Differentiation, Cell Separation, Cells, Cultured, Female, Flow Cytometry, Fluorescent Antibody Technique, Humans, Phenotype, Transplantation, Heterologous, Polymerase Chain Reaction, Mice, Inbred NOD, Mice, CD146 Antigen, Mesenchymal Stem Cells
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