The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Maturation of Embryonic Stem Cells Into Endothelial Cells in an In Vitro Model of Vasculogenesis
Abstract
A primitive vascular plexus is formed through coordinated regulation of differentiation, proliferation, migration, and cell-cell adhesion of endothelial cell (EC) progenitors. In this study, a culture system was devised to investigate the behavior of purified EC progenitors in vitro. Because Flk-1+ cells derived from ES cells did not initially express other EC markers, they were sorted and used as EC progenitors. Their in vitro differentiation into ECs, via vascular endothelial-cadherin (VE-cadherin)+ platelet-endothelial cell adhesion molecule-1 (PECAM-1)+ CD34−to VE-cadherin+ PECAM-1+CD34+ stage, occurred without exogenous factors, whereas their proliferation, particularly at low cell density, required OP9 feeder cells. On OP9 feeder layer, EC progenitors gave rise to sheet-like clusters of Flk-1+ cells, with VE-cadherin concentrated at the cell-cell junction. The growth was suppressed by Flt-1-IgG1 chimeric protein and dependent on vascular endothelial growth factor (VEGF) but not placenta growth factor (PIGF). Further addition of VEGF resulted in cell dispersion, indicating the role of VEGF in the migration of ECs as well as their proliferation. Cell-cell adhesion of ECs in this culture system was mediated by VE-cadherin. Thus, the culture system described here is useful in dissecting the cellular events of EC progenitors that occur during vasculogenesis and in investigating the molecular mechanisms underlying these processes.
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Francis, S. E., Goh, K. L., Hodivala-Dilke, K., Bader, B. L., Stark, M., Davidson, D., & Hynes, R. O. (2002) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000016045.93313.f2 · Cited by 282
Hedgehog is required for murine yolk sac angiogenesis
Byrd, N., Becker, S., Maye, P., Narasimhaiah, R., St-Jacques, B., Zhang, X., McMahon, J., McMahon, A., & Grabel, L. (2002) · Development · doi:10.1242/dev.129.2.361 · Cited by 257
Tissue engineering of blood vessel
Zhang, W. J., Liu, W., Cui, L., & Cao, Y. (2007) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2007.00099.x · Cited by 217
Requirement of Runx1/AML1/PEBP2αB for the generation of haematopoietic cells from endothelial cells
Yokomizo, T., Ogawa, M., Osato, M., Kanno, T., Yoshida, H., Fujimoto, T., Fraser, S., Nishikawa, S., Okada, H., Satake, M., et al. (2001) · Genes to Cells · doi:10.1046/j.1365-2443.2001.00393.x · Cited by 176
Self-citation
Metadata
- Authors
- Hirashima, M., Kataoka, H., Nishikawa, S., Matsuyoshi, N., & Nishikawa, S.-I.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1999-02-15
- Volume
- 93
- Issue
- 4
- Pages
- 1253-1263
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1606473945
- Cited by
- 276
- References
- 55
- FWCI
- 5.98
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hirashima, M., Kataoka, H., Nishikawa, S., Matsuyoshi, N., & Nishikawa, S.-I. (1999). Maturation of Embryonic Stem Cells Into Endothelial Cells in an In Vitro Model of Vasculogenesis. Blood. https://doi.org/10.1182/blood.v93.4.1253
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