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Identification of the transcription factor MAZ as a regulator of erythropoiesis

Deen, Darya, Butter, Falk, Daniels, Deborah E., Ferrer-Vicens, Ivan, Ferguson, Daniel C. J., Holland, Michelle L., Samara, Vasiliki, Sloane-Stanley, Jacqueline A., Ayyub, Helena, Mann, Matthias, Frayne, Jan, Garrick, David, Vernimmen, Douglas·2021·Blood Advances
DOI 10.1182/bloodadvances.2021004609
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Abstract

Erythropoiesis requires a combination of ubiquitous and tissue-specific transcription factors (TFs). Here, through DNA affinity purification followed by mass spectrometry, we have identified the widely expressed protein MAZ (Myc-associated zinc finger) as a TF that binds to the promoter of the erythroid-specific human α-globin gene. Genome-wide mapping in primary human erythroid cells revealed that MAZ also occupies active promoters as well as GATA1-bound enhancer elements of key erythroid genes. Consistent with an important role during erythropoiesis, knockdown of MAZ reduces α-globin expression in K562 cells and impairs differentiation in primary human erythroid cells. Genetic variants in the MAZ locus are associated with changes in clinically important human erythroid traits. Taken together, these findings reveal the zinc-finger TF MAZ to be a previously unrecognized regulator of the erythroid differentiation program.

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Cited by

Metadata

Authors
Deen, D., Butter, F., Daniels, D. E., Ferrer-Vicens, I., Ferguson, D. C. J., Holland, M. L., Samara, V., Sloane-Stanley, J. A., Ayyub, H., Mann, M., Frayne, J., Garrick, D., & Vernimmen, D.
Venue
Blood Advances
Publisher
American Society of Hematology
Published
2021-08-05
Volume
5
Issue
15
Pages
3002-3015
ISSN
  • 2473-9529
  • 2473-9537
DOI
10.1182/bloodadvances.2021004609
Cited by
15
References
4
Language
en
Update notices
erratum (2022-03-16)

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