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Cited by 172Mitochondrial Function and PathologyChronic Obstructive Pulmonary Disease (COPD) ResearchMuscle Physiology and Disorders

Abnormal Mitochondrial Function in Locomotor and Respiratory Muscles of COPD Patients

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Puente-Maestu, L., Pérez-Parra, J., Godoy, R., Moreno, N., Tejedor, A., González-Aragoneses, F., Bravo, J-L., Villar Álvarez, F. +2 more·2009·European Respiratory Journal
DOI 10.1183/09031936.00112408
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Abstract

Several cellular and molecular alterations have been described in skeletal and respiratory muscles of patients with chronic obstructive pulmonary disease (COPD), but information on potential abnormalities of mitochondrial function is scarce. The aim of the present study was to investigate mitochondrial function in the vastus lateralis (VL) and external intercostalis (EI) of COPD patients. Biopsies from VL and EI were obtained during surgery for lung cancer in 13 patients with mild to moderate COPD (age 68±6 yrs, forced expiratory volume in one second (FEV 1) 66±15% predicted) and 19 control subjects (age 67±9 yrs, FEV 1 95±18% pred). State 3 and 4 mitochondrial oxygen consumption (V′O 2,m), ATP synthesis, citrate synthase, cytochrome oxidase (COX) and complex I–III activities, as well as reactive oxygen species (ROS) production, were determined. In COPD patients, in both muscles, COX activity (VL: COPD 3.0±0.8 versus control 2.0±0.8; EI: 3.7±1.6 versus 2.4±0.9 μmol·min−1·mg−1) and ROS production (VL: 1,643±290 versus 1,285±468; EI: 1,033±210 versus 848±288 arbitrary units) were increased, whereas state 3 V′O 2,m was reduced (VL: 2.9±0.3 versus 3.6±0.4; EI: 3.6±0.3 versus 4.1±0.4 mmol·min−1·kg−1). Skeletal muscle mitochondria of patients with chronic obstructive pulmonary disease show electron transport chain blockade and excessive production of reactive oxygen species. The concurrent involvement of both vastus lateralis and external intercostalis suggests a systemic (rather than a local) mechanism(s) already occurring in relatively early stages (Global Initiative for Chronic Obstructive Lung Disease stage II) of the disease.

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  • Some authors have found increased COX activity in COPD patients with resting hypoxemia and with exercise hypoxemia, while others have not found any significant increase.

    “While some authors have found an increased COX activity in COPD patients with resting hypoxemia [96] and with exercise hypoxemia [97], others have not found any significant increase [98].”

    From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [97]

    The citation [98] supports the clause saying that others found no significant increase in COX activity. In the Mitochondrial function section, this paper instead reports significantly higher COX activity in COPD patients than in controls in both muscles studied; the Previous studies section says its results agree with an earlier report of increased COX activity. The supplied passages do not give page numbers. (LLM, conf=0.93)

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Authors
Puente-Maestu, L., Pérez-Parra, J., Godoy, R., Moreno, N., Tejedor, A., González-Aragoneses, F., Bravo, J.-L., Villar Álvarez, F., Camaño, S., & Agustí, A.
Affiliations
  • Hospital Universitario Son Dureta (ES)
  • Hospital General Universitario Gregorio Marañón (ES)
  • Instituto de Salud Carlos III (ES)
ORCID
Venue
European Respiratory Journal
Publisher
European Respiratory Society (ERS)
Published
2009-01-07
Volume
33
Issue
5
Pages
1045-1052
ISSN
  • 0903-1936 (ISSN-L)
  • 1399-3003
DOI
10.1183/09031936.00112408
OpenAlex
W2118581168
Cited by
172
References
5
Citations by year
2026: 3 · 2025: 8 · 2024: 8 · 2023: 4 · 2022: 8 · 2021: 10 · 2020: 15 · 2019: 11 · 2018: 9 · 2017: 12 · 2016: 10 · 2015: 8 · 2014: 14 · 2013: 13 · 2012: 13
FWCI
5.21
Type
journal article
Language
en
Open access
Locations
  • European Respiratory Journal (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
Keywords
chronic obstructive pulmonary disease, mitochondrial function, skeletal muscle, respiratory muscles, vastus lateralis, cytochrome c oxidase, reactive oxygen species, mitochondrial oxygen consumption, electron transport chain, ATP production, citrate synthase
MeSH terms
Adenosine Triphosphate, Aged, Biopsy, Citrate (si)-Synthase, Electron Transport Complex IV, Female, Forced Expiratory Volume, Humans, Male, Mitochondria, Muscle, Oxygen Consumption, Respiratory Muscles, Spirometry, Succinate Cytochrome c Oxidoreductase, Reactive Oxygen Species, Muscle, Skeletal, Pulmonary Disease, Chronic Obstructive

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