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Cited by 42Muscle Physiology and DisordersChronic Obstructive Pulmonary Disease (COPD) ResearchMitochondrial Function and Pathology

Uncoupling Protein-3 Content Is Decreased in Peripheral Skeletal Muscle of Patients with COPD

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Gosker, H.R., Schrauwen, P., Hesselink, M.K.C., Schaart, G., van der Vusse, G.J., Wouters, E.F.M., Schols, A.M.W.J.·2003·European Respiratory Journal
DOI 10.1183/09031936.03.00089802
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Abstract

Mechanical efficiency is reduced in patients with chronic obstructive pulmonary disease (COPD). Furthermore, altered fibre-type distribution and metabolic profile has been observed in peripheral skeletal muscle of COPD patients. Since skeletal muscular uncoupling protein‐3 (UCP3) has been implicated in the regulation of energy metabolism, the aim of this study was to assess UCP3 in peripheral skeletal muscle of COPD patients and healthy controls. A total of 16 COPD patients and 11 healthy age-matched control subjects were studied. Mechanical efficiency was measured by means of cycle ergometry. Biopsies were taken from the vastus lateralis, and UCP3 and cytochrome c(as a marker for mitochondrial content) levels were assessed by Western blotting. Muscle fibre types and metabolic profile were examined histochemically. UCP3 levels were markedly decreased in COPD compared to controls. In COPD patients, there was a positive correlation between UCP3 content and the forced expiratory volume in one second. UCP3 content was not related to mechanical efficiency, or other muscular data such as fibre types, markers of oxidative/glycolytic energy metabolism or cytochrome c. The authors of this study conclude that uncoupling protein‐3 content is decreased in peripheral skeletal muscle of patients with chronic obstructive pulmonary disease and is related to disease severity, but not to mechanical efficiency. The low uncoupling protein‐3 content is independent of the loss of oxidative capacity observed in these patients.

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Crossref · 10.1183/09031936.03.00089802

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  • Increased lipid peroxidation has been observed in COPD muscle, possibly favored by reduced UCP3 levels.

    “Increased lipid peroxidation has also been observed, possibly favored by reduced UCP3 levels [112].”

    From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [112]

    The Discussion (page unavailable) reports reduced UCP3 in COPD muscle and cites observations of an imbalance between oxygen species and antioxidant status. It does not report increased lipid peroxidation, and says whether reduced UCP3 is involved in defence against reactive oxygen species needs further examination. (LLM, conf=0.85)

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  • Two studies failed to report increased caspase-3 expression in COPD patients.

    “Noteworthy, these two studies failed to report an increased caspase-3 expression in COPD patients [112,113].”

    From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [112]

    The Methods and Results describe measurements of UCP3, cytochrome c, muscle fibre characteristics, and exercise-related outcomes, but do not report caspase-3 expression. A search of our copy for caspase-3 and apoptosis found no relevant result, so this paper cannot substantiate a finding of no increase in caspase-3. (LLM, conf=0.76)

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  • Agusti and colleagues observed increased myonuclear fragmentation in muscle cells of COPD patients, whereas Gosker and colleagues observed unchanged myonuclear fragmentation.

    “Since the early reports of Agusti and colleagues and Gosker and colleagues, who observed, respectively, an increased and unchanged myonuclear fragmentation in muscle cells [111,112]”

    From Main Pathogenic Mechanisms and Recent Advances in COPD Peripheral Skeletal Muscle Wasting (2023) · cites this paper as [112]

    The Methods describe muscle-fibre staining and protein measurements, while the Results report fibre types and UCP3 content; neither section reports myonuclear fragmentation. Searches for myonuclear fragmentation and apoptosis-related nuclear measures found no passage addressing whether fragmentation was unchanged in COPD patients. Page numbers were unavailable in our copy. (LLM, conf=0.76)

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Research works printed but not linked: 3

  1. Bergstrom L. Muscle electrolytes in man. Determination by neutron activation analysis on needle biopsy specimens. A study on normal subjects, kidney patients, and patients with chronic diarrhea. Scand J Clin Lab Invest 1962; 68: 1-110.
  2. Gosker HR, van Mameren H, van Dijk PJ, et al. Skeletal muscle fibre type shifting and metabolic profile in patients with COPD. Eur Respir J 2002; 19: 617-626.
  3. Pouw EM, Schols AMWJ, Vusse van der GJ, Wouters EFM. Elevated inosine monophosphate levels in resting muscle of patients with stable COPD. Am J Respir Crit Care Med 1998; 157: 453-457.

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Authors
Gosker, H. R., Schrauwen, P., Hesselink, M. K. C., Schaart, G., van der Vusse, G. J., Wouters, E. F. M., & Schols, A. M. W. J.
Affiliations
Maastricht University (NL)
ORCID
Venue
European Respiratory Journal
Publisher
European Respiratory Society (ERS)
Published
2003-07
Volume
22
Issue
1
Pages
88-93
ISSN
  • 0903-1936 (ISSN-L)
  • 1399-3003
DOI
10.1183/09031936.03.00089802
OpenAlex
W2143924994
Cited by
42
References
49
Citations by year
2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 2
FWCI
1.96
Type
journal article
Language
en
Open access
Locations
  • European Respiratory Journal (publishedVersion)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion, other-oa)
  • Research Publications (Maastricht University) (submittedVersion, other-oa)
Funders
  • Eli Lilly and Company
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
Keywords
UCP3, chronic obstructive pulmonary disease, skeletal muscle, mechanical efficiency, muscle fiber type, oxidative metabolism, cytochrome c, mitochondrial content, disease severity, vastus lateralis, energy metabolism
MeSH terms
Uncoupling Protein 3, Aged, Analysis of Variance, Carrier Proteins, Energy Metabolism, Exercise Test, Female, Humans, Immunohistochemistry, Ion Channels, Male, Middle Aged, Blotting, Western, Case-Control Studies, Muscle, Skeletal, Mitochondrial Proteins, Pulmonary Disease, Chronic Obstructive

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