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Acetylated microtubules are required for fusion of autophagosomes with lysosomes

Xie, Rui, Nguyen, Susan, McKeehan, Wallace L, Liu, Leyuan·2010·BMC Cell Biology
DOI 10.1186/1471-2121-11-89PMC PMC2995476
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Abstract

Background: Autophagy is a dynamic process during which isolation membranes package substrates to form autophagosomes that are fused with lysosomes to form autolysosomes for degradation. Although it is agreed that the LC3II-associated mature autophagosomes move along microtubular tracks, it is still in dispute if the conversion of LC3I to LC3II before autophagosomes are fully mature and subsequent fusion of mature autophagosomes with lysosomes require microtubules. Results: We use biochemical markers of autophagy and a collection of microtubule interfering reagents to test the question. Results show that interruption of microtubules with either microtubule stabilizing paclitaxel or destabilizing nocodazole similarly impairs the conversion of LC3I to LC3II, but does not block the degradation of LC3II-associated autophagosomes. Acetylation of microtubules renders them resistant to nocodazole treatment. Treatment with vinblastine that causes depolymerization of both non-acetylated and acetylated microtubules results in impairment of both LC3I-LC3II conversion and LC3II-associated autophagosome fusion with lysosomes. Conclusions: Acetylated microtubules are required for fusion of autophagosomes with lysosomes to form autolysosomes.

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Metadata

Authors
Xie, R., Nguyen, S., McKeehan, W. L., & Liu, L.
Venue
BMC Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2010-11-22
Volume
11
Issue
1
ISSN
1471-2121
DOI
10.1186/1471-2121-11-89
PMC
PMC2995476
Cited by
195
References
11
Language
en

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