CoChat
Sign Up
Cited by 318Bacterial biofilms and quorum sensingVibrio bacteria research studiesAquaculture disease management and microbiota

The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.

Cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in Vibrio spp. by decreasing the DNA-binding activity of the quorum sensing response regulator LuxR

Open access
Brackman, Gilles, Defoirdt, Tom, Miyamoto, Carol, Bossier, Peter, Van Calenbergh, Serge, Nelis, Hans, Coenye, Tom·2008·BMC Microbiology
DOI 10.1186/1471-2180-8-149PMC PMC2551610
Open this paper in CoChat — free accountOpen PDF

Abstract

BACKGROUND: To date, only few compounds targeting the AI-2 based quorum sensing (QS) system are known. In the present study, we screened cinnamaldehyde and substituted cinnamaldehydes for their ability to interfere with AI-2 based QS. The mechanism of QS inhibition was elucidated by measuring the effect on bioluminescence in several Vibrio harveyi mutants. We also studied in vitro the ability of these compounds to interfere with biofilm formation, stress response and virulence of Vibrio spp. The compounds were also evaluated in an in vivo assay measuring the reduction of Vibrio harveyi virulence towards Artemia shrimp. RESULTS: Our results indicate that cinnamaldehyde and several substituted derivatives interfere with AI-2 based QS without inhibiting bacterial growth. The active compounds neither interfered with the bioluminescence system as such, nor with the production of AI-2. Study of the effect in various mutants suggested that the target protein is LuxR. Mobility shift assays revealed a decreased DNA-binding ability of LuxR. The compounds were further shown to (i) inhibit biofilm formation in several Vibrio spp., (ii) result in a reduced ability to survive starvation and antibiotic treatment, (iii) reduce pigment and protease production in Vibrio anguillarum and (iv) protect gnotobiotic Artemia shrimp against virulent Vibrio harveyi BB120. CONCLUSION: Cinnamaldehyde and cinnamaldehyde derivatives interfere with AI-2 based QS in various Vibrio spp. by decreasing the DNA-binding ability of LuxR. The use of these compounds resulted in several marked phenotypic changes, including reduced virulence and increased susceptibility to stress. Since inhibitors of AI-2 based quorum sensing are rare, and considering the role of AI-2 in several processes these compounds may be useful leads towards antipathogenic drugs.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1186/1471-2180-8-149

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1 Cinnamaldehyde and cinnamaldehyde derivatives used in this study.
Figure 1 Cinnamaldehyde and cinnamaldehyde derivatives used in this study.CC BY · PubMed Central
Figure 2 Effect of cinnamaldehyde and cinnamaldehyde derivatives on AI-2 based QS. Bioluminescence in Vibrio harveyi BB170 as a function of the concentration of cinnamaldehyde and cinnamaldehyde derivatives. Bioluminescence measurements were performed 6 h after the addition of the compounds. Bioluminescence of the control (without addition of compound) was set at 100% and the responses for other samples were normalised accordingly. The error bars represent the standard deviation.
Figure 2 Effect of cinnamaldehyde and cinnamaldehyde derivatives on AI-2 based QS. Bioluminescence in Vibrio harveyi BB170 as a function of the concentration of cinnamaldehyde and cinnamaldehyde derivatives. Bioluminescence measurements were performed 6 h after the addition of the compounds. Bioluminescence of the control (without addition of compound) was set at 100% and the responses for other samples were normalised accordingly. The error bars represent the standard deviation.CC BY · PubMed Central
Figure 3 Effect of cinnamaldehyde and 2-NO2-cinnamaldehyde on the bioluminescence of wild type Vibrio harveyi BB120 and the different Vibrio harveyi QS mutants. The percentage of bioluminescence of the Vibrio harveyi wild type BB120 and the mutants MM30, JAF553, JAF483 and BNL258 with 100 μM cinnamaldehyde (white bars) or 100 μM 2-NO2-cinnamaldehyde (black bars) are presented. Measurements were performed 6 h after the addition of the compounds. Bioluminescence of the control (without addition of compound) was set at 100% and the response for the other samples were normalised accordingly. The error bars represent the standard deviation.
Figure 3 Effect of cinnamaldehyde and 2-NO2-cinnamaldehyde on the bioluminescence of wild type Vibrio harveyi BB120 and the different Vibrio harveyi QS mutants. The percentage of bioluminescence of the Vibrio harveyi wild type BB120 and the mutants MM30, JAF553, JAF483 and BNL258 with 100 μM cinnamaldehyde (white bars) or 100 μM 2-NO2-cinnamaldehyde (black bars) are presented. Measurements were performed 6 h after the addition of the compounds. Bioluminescence of the control (without addition of compound) was set at 100% and the response for the other samples were normalised accordingly. The error bars represent the standard deviation.CC BY · PubMed Central
Figure 4 LuxR DNA-binding as determined by mobility shifts and LuxR protein levels as determined by SDS-PAGE. A. Autoradiograph after 5% polacrylamide gel electrophoresis of 32P-labelled LuxR promoter DNA containing the LuxR binding sites, mixed with purified LuxR in the presence (0.19, 0.75 and 1.9 mM) and absence of cinnamaldehyde. B. SDS-PAGE of purified LuxR protein in the presence (0.19, 0.75 and 1.9 mM) or absence of cinnamaldehyde.
Figure 4 LuxR DNA-binding as determined by mobility shifts and LuxR protein levels as determined by SDS-PAGE. A. Autoradiograph after 5% polacrylamide gel electrophoresis of 32P-labelled LuxR promoter DNA containing the LuxR binding sites, mixed with purified LuxR in the presence (0.19, 0.75 and 1.9 mM) and absence of cinnamaldehyde. B. SDS-PAGE of purified LuxR protein in the presence (0.19, 0.75 and 1.9 mM) or absence of cinnamaldehyde.CC BY · PubMed Central
Figure 5 Effect of cinnamaldehyde and cinnamaldehyde derivatives on the protease activity of Vibrio anguillarum LMG 4411. Cinnamaldehyde and cinnamaldehyde derivatives were tested at 100 μM, except 4-NO2-cinnamaldehyde (25 μM). The effect of cinnamaldehyde or cinnamaldehyde derivatives on protease activity was compared to an untreated control. The error bars represent the standard deviation. *: Signal significantly different from the control (p < 0.05).
Figure 5 Effect of cinnamaldehyde and cinnamaldehyde derivatives on the protease activity of Vibrio anguillarum LMG 4411. Cinnamaldehyde and cinnamaldehyde derivatives were tested at 100 μM, except 4-NO2-cinnamaldehyde (25 μM). The effect of cinnamaldehyde or cinnamaldehyde derivatives on protease activity was compared to an untreated control. The error bars represent the standard deviation. *: Signal significantly different from the control (p < 0.05).CC BY · PubMed Central
Figure 6 Effect of cinnamaldehyde and 2-NO2-cinnamaldehyde on the pigment production of Vibrio anguillarum LMG 4411. Cinnamaldehyde and 2-NO2-cinnamaldehyde were tested at 100 μM. Vibrio anguillarum LMG 4411 was allowed to produce pigment in the absence (solid symbol) or presence of cinnamaldehyde (open symbol, square) or 2-NO2-cinnamaldehyde (open symbol, triangle). Three ml samples were taken at multiple time points. The effect of cinnamaldehyde or 2-NO2-cinnamaldehyde on pigment production was estimated by measuring the absorbance at 405 nm. The error bars represent the standard deviation.
Figure 6 Effect of cinnamaldehyde and 2-NO2-cinnamaldehyde on the pigment production of Vibrio anguillarum LMG 4411. Cinnamaldehyde and 2-NO2-cinnamaldehyde were tested at 100 μM. Vibrio anguillarum LMG 4411 was allowed to produce pigment in the absence (solid symbol) or presence of cinnamaldehyde (open symbol, square) or 2-NO2-cinnamaldehyde (open symbol, triangle). Three ml samples were taken at multiple time points. The effect of cinnamaldehyde or 2-NO2-cinnamaldehyde on pigment production was estimated by measuring the absorbance at 405 nm. The error bars represent the standard deviation.CC BY · PubMed Central
Figure 7 Effect of cinnamaldehyde and cinnamaldehyde derivatives on Vibrio spp. biofilms. Biomass was quantified through CV staining. Cell-viability was quantified through CTB staining. CV signals are presented as a percentage compared to 100% control not receiving treatment (black bars = Vibrio vulnificus; white bars = Vibrio anguillarum). CTB signals are presented as a percentage compared to a 100% control not receiving treatment (vertical striped bars = Vibrio vulnificus; horizontal striped bars = Vibrio anguillarum). *: Signal significantly different compared to 100% control (p < 0.05).
Figure 7 Effect of cinnamaldehyde and cinnamaldehyde derivatives on Vibrio spp. biofilms. Biomass was quantified through CV staining. Cell-viability was quantified through CTB staining. CV signals are presented as a percentage compared to 100% control not receiving treatment (black bars = Vibrio vulnificus; white bars = Vibrio anguillarum). CTB signals are presented as a percentage compared to a 100% control not receiving treatment (vertical striped bars = Vibrio vulnificus; horizontal striped bars = Vibrio anguillarum). *: Signal significantly different compared to 100% control (p < 0.05).CC BY · PubMed Central
Figure 8 Effect of cinnamaldehyde and 2-NO2-cinnamaldehyde on the survival of Artemia. White bars represent the survival of Artemia without challenge with Vibrio harveyi BB120. Black bars represent the percentage survival of Artemia after challenge with Vibrio harveyi BB120 in untreated conditions. Striped bars represent the percentage of survival of Artemia after challenge with Vibrio harveyi BB120 when treated with cinnamaldehyde or 2-NO2-cinnamaldehyde (horizontal: 100 μM stripes; vertical stripes: 150 μM, respectively). *: Survival significantly different from the treatment with pathogen alone (p < 0.01).
Figure 8 Effect of cinnamaldehyde and 2-NO2-cinnamaldehyde on the survival of Artemia. White bars represent the survival of Artemia without challenge with Vibrio harveyi BB120. Black bars represent the percentage survival of Artemia after challenge with Vibrio harveyi BB120 in untreated conditions. Striped bars represent the percentage of survival of Artemia after challenge with Vibrio harveyi BB120 when treated with cinnamaldehyde or 2-NO2-cinnamaldehyde (horizontal: 100 μM stripes; vertical stripes: 150 μM, respectively). *: Survival significantly different from the treatment with pathogen alone (p < 0.01).CC BY · PubMed Central
Figure 9 Effect of cinnamaldehyde on Vibrio spp. starvation response. The cells were allowed to starve in the presence (horizontal striped bars = Vibrio vulnificus; vertical striped bars = Vibrio anguillarum) and absence (black bars = Vibrio vulnificus; white bars = Vibrio anguillarum) of cinnamaldehyde. The number of CFU/ml was determined after 24 h and 48 h on TSA plates containing 2% NaCl. Data are presented as a percentage of the initial count. Error bars represent standard deviations.
Figure 9 Effect of cinnamaldehyde on Vibrio spp. starvation response. The cells were allowed to starve in the presence (horizontal striped bars = Vibrio vulnificus; vertical striped bars = Vibrio anguillarum) and absence (black bars = Vibrio vulnificus; white bars = Vibrio anguillarum) of cinnamaldehyde. The number of CFU/ml was determined after 24 h and 48 h on TSA plates containing 2% NaCl. Data are presented as a percentage of the initial count. Error bars represent standard deviations.CC BY · PubMed Central
Figure 10 Effect of cinnamaldehyde on antibiotic susceptibility of Vibrio vulnificus LMG 16867. Effects of chloramphenicol (squares) and doxycycline (triangles) on the growth of Vibrio vulnificus LMG16867 in the presence (open symbols) and absence (solid symbols) of cinnamaldehyde (100 μM) are presented. The absorbance at 590 nm was measured after 24 h of growth. Error bars represent standard deviations.
Figure 10 Effect of cinnamaldehyde on antibiotic susceptibility of Vibrio vulnificus LMG 16867. Effects of chloramphenicol (squares) and doxycycline (triangles) on the growth of Vibrio vulnificus LMG16867 in the presence (open symbols) and absence (solid symbols) of cinnamaldehyde (100 μM) are presented. The absorbance at 590 nm was measured after 24 h of growth. Error bars represent standard deviations.CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 49 references

Cited by

Sorted by citations

Items 201–300 of 318

Related

See all

Metadata

Authors
Brackman, G., Defoirdt, T., Miyamoto, C., Bossier, P., Van Calenbergh, S., Nelis, H., & Coenye, T.
Affiliations
  • Ghent University (BE)
  • McGill University (CA)
Corresponding author
Coenye, Tom
ORCID
Venue
BMC Microbiology
Publisher
Springer Science and Business Media LLC
Published
2008-09-16
Volume
8
Issue
1
Pages
149-149
ISSN
1471-2180 (ISSN-L)
DOI
10.1186/1471-2180-8-149
PMC
PMC2551610
OpenAlex
W2087415918
Cited by
317
References
49
Citations by year
2026: 11 · 2025: 15 · 2024: 12 · 2023: 18 · 2022: 24 · 2021: 22 · 2020: 25 · 2019: 20 · 2018: 25 · 2017: 14 · 2016: 22 · 2015: 25 · 2014: 24 · 2013: 19 · 2012: 10
FWCI
4.25
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • BMC Microbiology (publishedVersion, cc-by)
  • Flanders Marine Institute (Flanders Marine Institute) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Fonds Wetenschappelijk Onderzoek
  • Vlaamse regering
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Immunology and Microbiology › Microbiology › Vibrio bacteria research studies
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
Keywords
cinnamaldehyde, cinnamaldehyde derivatives, quorum sensing inhibition, autoinducer-2, LuxR, Vibrio harveyi, Vibrio anguillarum, virulence reduction, biofilm formation, stress response, DNA-binding activity, bioluminescence
MeSH terms
Acrolein, Animals, Anti-Bacterial Agents, Artemia, DNA, Mutation, Peptide Hydrolases, Pigments, Biological, Repressor Proteins, Vibrio, Virulence, Trans-Activators, Biofilms, Luminescence, Quorum Sensing

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review