Differential effectiveness of Serratia plymuthica IC1270-induced systemic resistance against hemibiotrophic and necrotrophic leaf pathogens in rice
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BACKGROUND: Induced resistance is a state of enhanced defensive capacity developed by a plant reacting to specific biotic or chemical stimuli. Over the years, several forms of induced resistance have been characterized, including systemic acquired resistance, which is induced upon localized infection by an avirulent necrotizing pathogen, and induced systemic resistance (ISR), which is elicited by selected strains of nonpathogenic rhizobacteria. However, contrary to the relative wealth of information on inducible defense responses in dicotyledoneous plants, our understanding of the molecular mechanisms underlying induced resistance phenomena in cereal crops is still in its infancy. Using a combined cytomolecular and pharmacological approach, we analyzed the host defense mechanisms associated with the establishment of ISR in rice by the rhizobacterium Serratia plymuthica IC1270. RESULTS: In a standardized soil-based assay, root treatment with IC1270 rendered foliar tissues more resistant to the hemibiotrophic pathogen Magnaporthe oryzae, causal agent of the devastating rice blast disease. Analysis of the cytological and biochemical alterations associated with restriction of fungal growth in IC1270-induced plants revealed that IC1270 primes rice for enhanced attacker-induced accumulation of reactive oxygen species (ROS) and autofluorescent phenolic compounds in and near epidermal cells displaying dense cytoplasmic granulation. Similar, yet more abundant, phenotypes of hypersensitively dying cells in the vicinity of fungal hyphae were evident in a gene-for-gene interaction with an avirulent M. oryzae strain, suggesting that IC1270-inducible ISR and R protein conditioned effector-triggered immunity (ETI) target similar defense mechanisms. Yet, this IC1270-inducible ISR response seems to act as a double-edged sword within the rice defense network as induced plants displayed an increased vulnerability to the necrotrophic pathogens Rhizoctonia solani and Cochliobolus miyabeanus. Artificial enhancement of ROS levels in inoculated leaves faithfully mimicked the opposite effects of IC1270 bacteria on aforementioned pathogens, suggesting a central role for oxidative events in the IC1270-induced resistance mechanism. CONCLUSION: Besides identifying ROS as modulators of antagonistic defense mechanisms in rice, this work reveals the mechanistic similarities between S. plymuthica-mediated ISR and R protein-dictated ETI and underscores the importance of using appropriate innate defense mechanisms when breeding for broad-spectrum rice disease resistance.
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ISR describes a systemic resistance effect triggered by beneficial root-colonizing rhizobacteria in distal not-challenged plant parts of monocotyledons and dicotyledons.
“ISR describes a systemic resistance effect triggered by beneficial root-colonizing rhizobacteria in distal not-challenged plant parts of monocotyledons and dicotyledons (De Vleesschauwer et al., 2009;Pieterse et al., 2014).”
From Intensify production, transform biomass to energy and novel goods and protect soils in Europe—A vision how to mobilize marginal lands (2018) · cites this paper as De Vleesschauwer et al., 2009
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- Authors
- De Vleesschauwer, D., Chernin, L., & Höfte, M. M.
- Affiliations
- Hebrew University of Jerusalem (IL)
- Ghent University (BE)
- Corresponding author
- Höfte, Monica M
- ORCID
- Höfte, Monica M
- Venue
- BMC Plant Biology
- Published
- 2009-01-22
- Volume
- 9
- Issue
- 1
- Pages
- 9-9
- ISSN
- 1471-2229 (ISSN-L)
- PMC
- PMC2650696
- OpenAlex
- W1963735419
- Cited by
- 77
- References
- 85
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 3 · 2022: 6 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 9 · 2016: 8 · 2015: 3 · 2014: 2 · 2013: 8 · 2012: 13
- FWCI
- 9.26
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- BMC Plant Biology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- Funders
- Universiteit Gent
- Bijzonder Onderzoeksfonds UGent
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Disease Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Keywords
- induced systemic resistance, rice, Magnaporthe oryzae, rice blast, Rhizoctonia solani, Cochliobolus miyabeanus, reactive oxygen species, effector-triggered immunity, R proteins, gene-for-gene interaction, plant defense priming, necrotrophic pathogens, hemibiotrophic pathogens, phenolic compounds
- MeSH terms
- Immunity, Innate, Oxidation-Reduction, Plant Diseases, Rhizoctonia, Oryza, Serratia, Reactive Oxygen Species, Oxidative Stress, Plant Leaves, Plant Roots, Magnaporthe
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