Cited by 157Essential Oils and Antimicrobial ActivityAntimicrobial Resistance in StaphylococcusAntibiotic Resistance in Bacteria

Antibacterial activity against β- lactamase producing Methicillin and Ampicillin-resistants Staphylococcus aureus: fractional Inhibitory Concentration Index (FICI) determination

Open access
Konaté, Kiessoun, Mavoungou, Jacques, Lepengué, Alexis, Aworet-Samseny, Raïssa RR, Hilou, Adama, Souza, Alain, Dicko, Mamoudou H, M’Batchi, Bertrand·2012·Annals of Clinical Microbiology and Antimicrobials

Abstract

BACKGROUND: The present study reports the antibacterial capacity of alkaloid compounds in combination with Methicillin and Ampicillin-resistants bacteria isolated from clinical samples. The resistance of different bacteria strains to the current antibacterial agents, their toxicity and the cost of the treatment have led to the development of natural products against the bacteria resistant infections when applied in combination with conventional antimicrobial drugs. METHOD: The antibacterial assays in this study were performed by using inhibition zone diameters, MIC, MBC methods, the time-kill assay and the Fractional Inhibitory Concentration Index (FICI) determination. On the whole, fifteen Gram-positive bacterial strains (MRSA/ARSA) were used. Negative control was prepared using discs impregnated with 10 % DMSO in water and commercially available Methicillin and Ampicillin from Alkom Laboratories LTD were used as positive reference standards for all bacterial strains. RESULTS: We noticed that the highest activities were founded with the combination of alkaloid compounds and conventional antibiotics against all bacteria strains. Then, results showed that after 7 h exposition there was no viable microorganism in the initial inoculums. CONCLUSION: The results of this study showed that alkaloid compounds in combination with conventional antibiotics (Methicillin, Ampicillin) exhibited antimicrobial effects against microorganisms tested. These results validate the ethno-botanical use of Cienfuegosia digitata Cav. (Malvaceae) in Burkina Faso. Moreover, this study demonstrates the potential of this herbaceous as a source of antibacterial agent that could be effectively used for future health care purposes.

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Crossref · 10.1186/1476-0711-11-18

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  • The advantages of disk-diffusion, mainly simplicity and low cost, have contributed to its common use for antimicrobial screening of plant extracts, essential oils and other drugs.

    “The above-mentioned advantages of this method, mainly simplicity and low cost, have contributed to its common use for the antimicrobial screening of plant extracts, essential oils and other drugs [20], [21], [22], [23].”

    From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [21]

    The Methods section (In vitro antibacterial activity; page not provided) describes a disc-diffusion assay using plant-derived alkaloid compounds and antibiotic controls, supporting the method’s use in antimicrobial testing. It does not say the method is simple or inexpensive, establish that those advantages explain its common use, or describe disc-diffusion screening of essential oils. (LLM, conf=0.86)

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  • Generally, the bactericidal effect is obtained with a lethality percentage of 90% for 6 h, which is equivalent to 99.9% of lethality for 24 h.

    “Generally, the bactericidal effect is obtained with a lethality percentage of 90% for 6 h, which is equivalent to 99.9% of lethality for 24 h [21].”

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Research works printed but not linked: 4

  1. Nacoulma OG Medicinal plants and their traditional uses in Burkina Faso 1996 University of Ouagadougou, Ph.D.Thesis 328
  2. Traoré R Contribution has the Study of the Adhesion of Enterobacteries of the Kinds Klebsiella proteus and K serratia with the Human epithelial Cells. Doct thesis science pharmaceutical 1993 Université libre de Bruxelles 158
  3. Ellof JN Which extractant should be used for the screening and isolation of antimicrobial components from plants J Ethnopharmacol 1998 60 1 8 9533426
  4. Bachi BB Rothrer S Factors influencing methicillin resistance in Staphylococci Arch Microbiol 2002 178 165 171 12189417

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Authors
Konaté, K., Mavoungou, J., Lepengué, A., Aworet-Samseny, R. R., Hilou, A., Souza, A., Dicko, M. H., & M’Batchi, B.
Affiliations
  • Université Joseph Ki-Zerbo (BF)
  • Université des Sciences et Techniques de Masuku (GA)
Corresponding author
Konaté, Kiessoun
Venue
Annals of Clinical Microbiology and Antimicrobials
Published
2012
Volume
11
Issue
1
Pages
18
ISSN
1476-0711 (ISSN-L)
OpenAlex
W2142832600
Cited by
155
References
29
Citations by year
2026: 11 · 2025: 18 · 2024: 16 · 2023: 21 · 2022: 17 · 2021: 20 · 2020: 12 · 2019: 10 · 2018: 10 · 2017: 4 · 2016: 5 · 2015: 2 · 2014: 5 · 2013: 4
FWCI
5.41
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Annals of Clinical Microbiology and Antimicrobials (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
Keywords
Staphylococcus aureus, methicillin resistance, ampicillin resistance, beta-lactamase, fractional inhibitory concentration index, combination antibiotic therapy, Malvaceae, antibacterial activity, time-kill assay, ethnobotany, Burkina Faso
MeSH terms
Alkaloids, Ampicillin, Ampicillin Resistance, Anti-Bacterial Agents, Bacterial Proteins, beta-Lactamases, Biological Assay, Drug Synergism, Methicillin, Microbial Sensitivity Tests, Plant Extracts, Methicillin Resistance, Malvaceae, Methicillin-Resistant Staphylococcus aureus, beta-Lactamase Inhibitors

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