The DOI matches its Crossref record; 47 of the 48 references it cites were found in a registry or an index.
Conjugated linoleic acid or omega 3 fatty acids increase mitochondrial biosynthesis and metabolism in skeletal muscle cells
Open accessAbstract
BACKGROUND: Polyunsaturated fatty acids are popular dietary supplements advertised to contribute to weight loss by increasing fat metabolism in liver, but the effects on overall muscle metabolism are less established. We evaluated the effects of conjugated linoleic acid (CLA) or combination omega 3 on metabolic characteristics in muscle cells. METHODS: Human rhabdomyosarcoma cells were treated with either DMSO control, or CLA or combination omega 3 for 24 or 48 hours. RNA was determined using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Mitochondrial content was determined using flow cytometry and immunohistochemistry. Metabolism was quantified by measuring extracellular acidification and oxygen consumption rates. RESULTS: Omega 3 significantly induced metabolic genes as well as oxidative metabolism (oxygen consumption), glycolytic capacity (extracellular acidification), and metabolic rate compared with control. Both treatments significantly increased mitochondrial content. CONCLUSION: Omega 3 fatty acids appear to enhance glycolytic, oxidative, and total metabolism. Moreover, both omega 3 and CLA treatment significantly increase mitochondrial content compared with control.
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“Similarly, a human cell line study also reported that omega-3 treatment significantly induced both glycolytic and oxidative metabolism and increased mitochondrial content [92].”
From Mitochondrial Adaptation in Skeletal Muscle: Impact of Obesity, Caloric Restriction, and Dietary Compounds (2024) · cites this paper as [92]
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“Furthermore, a combination of EPA and DHA in rhabdomyosarcoma cells reduced the ratio between the oxygen consumption rate: extracellular acidification rate indicating a shift towards an increase on glycolytic reliance [94].”
From Omega-3 Fatty Acids and Skeletal Muscle Health (2015) · cites this paper as [94]
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“Multiple studies have observed an increase in GLUT4 expression [93,94]”
From Omega-3 Fatty Acids and Skeletal Muscle Health (2015) · cites this paper as [94]
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- Duda MK, Xu WH, Tintinu A, O'Shea KM, Stanley WC: Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) Supplementation, but not alpha-Linolenic Acid, Elevates Plasma Adiponectin Concentration and Prevents Pressure Overload Induced Ventricular Dysfunction and Remodeling. Circulation. 2008, 118: S542-S542.
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Production of Conjugated-Linoleic Acids Using Rumen and Probiotic Bacteria
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Nardi, F. (2015) · Discovery Research Portal (University of Dundee)
Metadata
- Authors
- Vaughan, R. A., Garcia-Smith, R., Bisoffi, M., Conn, C. A., & Trujillo, K. A.
- Affiliations
- University of New Mexico (US)
- Corresponding author
- Vaughan, Roger A
- Venue
- Lipids in Health and Disease
- Published
- 2012-10-30
- Volume
- 11
- Issue
- 1
- Pages
- 142-142
- ISSN
- 1476-511X (ISSN-L)
- PMC
- PMC3515476
- OpenAlex
- W2117258334
- Cited by
- 112
- References
- 48
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 4 · 2023: 9 · 2022: 8 · 2021: 6 · 2020: 11 · 2019: 5 · 2018: 9 · 2017: 10 · 2016: 13 · 2015: 12 · 2014: 10 · 2013: 6 · 2012: 1
- FWCI
- 8.66
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Lipids in Health and Disease (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Fatty Acid Research and Health
- Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- conjugated linoleic acid, omega-3 fatty acids, mitochondrial biogenesis, skeletal muscle metabolism, rhabdomyosarcoma cells, glycolysis, oxidative metabolism, extracellular acidification, oxygen consumption, polyunsaturated fatty acids, dietary supplements
- MeSH terms
- Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Base Sequence, DNA, Fibronectins, Glycolysis, Heat-Shock Proteins, Humans, Mitochondria, Muscle, Oxygen Consumption, Transcription Factors, Tumor Cells, Cultured, Fatty Acids, Omega-3, Gene Expression, Muscle Fibers, Skeletal, Dietary Supplements, Linoleic Acids, Conjugated, Glucose Transporter Type 4
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