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Cited by 29Zebrafish Biomedical Research ApplicationsHematopoietic Stem Cell TransplantationDevelopmental Biology and Gene Regulation

Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model

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Martin, Corey S, Moriyama, Akemi, Zon, Leonard I·2011·Genome Medicine
DOI 10.1186/gm299PMC PMC3334548
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Abstract

The zebrafish model is rapidly gaining prominence in the study of development, hematopoiesis, and disease. The zebrafish provides distinct advantages over other vertebrate models during early embryonic development by producing transparent, externally fertilized embryos. Embryonic zebrafish are easily visualized and manipulated through microinjection, chemical treatment, and mutagenesis. These procedures have contributed to large-scale chemical, suppressor, and genetic screens to identify hematopoietic gene mutations. Genomic conservation and local synteny between the human and zebrafish genomes make genome-scale and epigenetic analysis of these mutations (by microarray, chromatin immunoprecipitation sequencing, and RNA sequencing procedures) powerful methods for translational research and medical discovery. In addition, large-scale screening techniques have resulted in the identification of several small molecules capable of rescuing hematopoietic defects and inhibiting disease. Here, we discuss the contributions of the zebrafish model to the understanding of hematopoiesis, hematopoietic stem cell development, and disease-related discovery. We also highlight the recent discovery of small molecules with clinical promise, such as dimethyl prostaglandin E2, 3F8, and thiazole-carboxamide 10A.

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  • Cluster RBC7 represents mature erythrocytes, characterized by high expression of band3 (slc4a1a).

    “Cluster RBC7, characterized by high expression of band3 (slc4a1a), was determined to represent mature erythrocytes (Martin et al., 2011).”

    From Deciphering the toxic effects of polystyrene nanoparticles on erythropoiesis at single-cell resolution (2025) · cites this paper as Martin et al., 2011

    The Genome comparison section discusses a red-blood-cell-specific transferrin receptor gene, and Implications for translational stem cell research mentions erythrocyte numbers. Neither identifies band3 (slc4a1a) as a marker of mature erythrocytes or discusses an RBC7 cluster. Searches for band3, slc4a1a, and mature erythrocyte markers found no passage addressing that point; page numbers were unavailable. (LLM, conf=0.75)

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Cited by

Sorted by citations

Authors
Martin, C. S., Moriyama, A., & Zon, L. I.
Affiliations
Boston Children's Hospital (US)
Corresponding author
Zon, Leonard I
ORCID
Zon, Leonard I
Venue
Genome Medicine
Publisher
Springer Science and Business Media LLC
Published
2011
Volume
3
Issue
1
Pages
83
ISSN
1756-994X (ISSN-L)
DOI
10.1186/gm299
PMC
PMC3334548
OpenAlex
W2111824108
Cited by
29
References
5
Citations by year
2025: 3 · 2024: 1 · 2020: 1 · 2019: 3 · 2018: 3 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 5 · 2012: 1
FWCI
1.69
Type
journal article
Language
en
Open access
Locations
  • Genome Medicine (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
hematopoietic stem cells, hematopoiesis, zebrafish model, chemical screen, suppressor screen, microinjection, small molecules, hematopoietic defects, epigenetics, translational research

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