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Transposon insertion sequencing reveals T4SS as the major genetic trait for conjugation transfer of multi-drug resistance pEIB202 from Edwardsiella

Liu, Yang, Gao, Yanan, Liu, Xiaohong, Liu, Qin, Zhang, Yuanxing, Wang, Qiyao, Xiao, Jingfan·2017·BMC Microbiology
DOI 10.1186/s12866-017-1013-7
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Research works printed but not linked: 53

  1. Lopatkin AJ, Huang S, Smith RP, Srimani JK, Sysoeva TA, Bewick S, Karig DK, You L. Antibiotics as a selective driver for conjugation dynamics. Nat Microbiol. 2016;1(6):16044. · DOI · Paper page
  2. Sorensen SJ, Bailey M, Hansen LH, Kroer N, Wuertz S. Studying plasmid horizontal transfer in situ: a critical review. Nat Rev Microbiol. 2005;3(9):700–10. · DOI · Paper page
  3. Maiden MC. Horizontal genetic exchange, evolution, and spread of antibiotic resistance in bacteria. Clin Infect Dis. 1998;27(Suppl 1):S12–20. · DOI
  4. Turner SL, Bailey MJ, Lilley AK, Thomas CM. Ecological and molecular maintenance strategies of mobile genetic elements. FEMS Microbiol Ecol. 2002;42(2):177–85. · doi:10.1111/j.1574-6941.2002.tb01007.x
  5. van Elsas JD, Bailey MJ. The ecology of transfer of mobile genetic elements. FEMS Microbiol Ecol. 2002;42(2):187–97. · DOI · Paper page
  6. Bruto M, James A, Petton B, Labreuche Y, Chenivesse S, Alunno-Bruscia M, Polz MF, Le Roux F. Vibrio crassostreae, a benign oyster colonizer turned into a pathogen after plasmid acquisition. ISME J. 2016; doi: 10.1038/ismej.2016.162 . · DOI · Paper page
  7. Rhodes G, Huys G, Swings J, McGann P, Hiney M, Smith P, Pickup RW. Distribution of oxytetracycline resistance plasmids between aeromonads in hospital and aquaculture environments: implication of Tn1721 in dissemination of the tetracycline resistance determinant tet a. Appl Environ Microbiol. 2000;66(9):3883–90. · DOI · Paper page
  8. Popa O, Dagan T. Trends and barriers to lateral gene transfer in prokaryotes. Curr Opin Microbiol. 2011;14(5):615–23. · DOI · Paper page
  9. Fronzes R, Christie PJ, Waksman G. The structural biology of type IV secretion systems. Nat Rev Microbiol. 2009;7(10):703–14. · DOI · Paper page
  10. Cascales E, Christie PJ. The versatile bacterial type IV secretion systems. Nat Rev Microbiol. 2003;1(2):137–49. · DOI · Paper page
  11. Backert S, Meyer TF. Type IV secretion systems and their effectors in bacterial pathogenesis. Curr Opin Microbiol. 2006;9(2):207–17. · DOI · Paper page
  12. Alvarez-Martinez CE, Christie PJ. Biological diversity of prokaryotic type IV secretion systems. Microbiol Mol Biol Rev. 2009;73(4):775–808. · DOI · Paper page
  13. Park SB, Aoki T, Jung TS. Pathogenesis of and strategies for preventing Edwardsiella tarda infection in fish. Vet Res. 2012;43(1):67. · doi:10.1186/1297-9716-43-67
  14. Tan YP, Zheng J, Tung SL, Rosenshine I, Leung KY. Role of type III secretion in Edwardsiella tarda virulence. Microbiology. 2005;151(Pt 7):2301–13. · DOI
  15. Okuda J, Kiriyama M, Suzaki E, Kataoka K, Nishibuchi M, Nakai T. Characterization of proteins secreted from a type III secretion system of Edwardsiella tarda and their roles in macrophage infection. Dis Aquat Org. 2009;84(2):115–21. · doi:10.3354/dao02033
  16. Hou M, Chen R, Yang D, Nunez G, Wang Z, Wang Q, Zhang Y, Liu Q. Identification and functional characterization of EseH, a new effector of the type III secretion system of Edwardsiella piscicida. Cell Microbiol. 2017;19(1):e12638. · doi:10.1111/cmi.12638
  17. Wang Q, Yang M, Xiao J, Wu H, Wang X, Lv Y, Xu L, Zheng H, Wang S, Zhao G, et al. Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS One. 2009;4(10):e7646. · DOI · Paper page
  18. Yang M, Lv Y, Xiao J, Wu H, Zheng H, Liu Q, Zhang Y, Wang Q. Edwardsiella comparative phylogenomics reveal the new intra/inter-species taxonomic relationships, virulence evolution and niche adaptation mechanisms. PLoS One. 2012;7(5):e36987. · DOI
  19. Lv Y, Xiao J, Liu Q, Wu H, Zhang Y, Wang Q. Systematic mutation analysis of two-component signal transduction systems reveals EsrA-EsrB and PhoP-PhoQ as the major virulence regulators in Edwardsiella tarda. Vet Microbiol. 2012;157(1–2):190–9. · doi:10.1016/j.vetmic.2011.12.018
  20. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) method. Methods. 2001;25(4):402–8. · DOI · Paper page
  21. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CR, Smith HO. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods. 2009;6(5):343–5. · DOI · Paper page
  22. Chao MC, Pritchard JR, Zhang YJ, Rubin EJ, Livny J, Davis BM, Waldor MK. High-resolution definition of the Vibrio cholerae essential gene set with hidden Markov model-based analyses of transposon-insertion sequencing data. Nucleic Acids Res. 2013;41(19):9033–48. · DOI
  23. Langmead B, Trapnell C, Pop M, Salzberg SL. Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009;10(3):R25. · DOI · Paper page
  24. Xiao J, Wang Q, Liu Q, Xu L, Wang X, Wu H, Zhang Y. Characterization of Edwardsiella tarda rpoS. effect on serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression. Appl Microbiol Biotechnol. 2009;83(1):151–60. · doi:10.1007/s00253-009-1924-9
  25. Yamaichi Y, Chao MC, Sasabe J, Clark L, Davis BM, Yamamoto N, Mori H, Kurokawa K, Waldor MK. High-resolution genetic analysis of the requirements for horizontal transmission of the ESBL plasmid from Escherichia coli O104:H4. Nucleic Acids Res. 2015;43(1):348–60. · DOI
  26. Bolger AM, Lohse M, Usadel B. Trimmomatic. a flexible trimmer for Illumina sequence data. Bioinformatics. 2014;30(15):2114–20. · DOI · Paper page
  27. Li H, Durbin R. Fast and accurate short read alignment with burrows-wheeler transform. Bioinformatics. 2009;25(14):1754–60. · DOI · Paper page
  28. Anders S, Huber W. Differential expression analysis for sequence count data. Genome Biol. 2010;11(10):R106. · DOI · Paper page
  29. Fernandez AI, Perez MJ, Rodriguez LA, Nieto TP. Surface phenotypic characteristics and virulence of Spanish isolates of Aeromonas salmonicida after passage through fish. Appl Environ Microbiol. 1995;61(5):2010–2. · doi:10.1128/aem.61.5.2010-2012.1995
  30. Pritchard JR, Chao MC, Abel S, Davis BM, Baranowski C, Zhang YJ, Rubin EJ, Waldor MK. ARTIST: high-resolution genome-wide assessment of fitness using transposon-insertion sequencing. PLoS Genet. 2014;10(11):e1004782. · DOI
  31. Low HH, Gubellini F, Rivera-Calzada A, Braun N, Connery S, Dujeancourt A, Lu F, Redzej A, Fronzes R, Orlova EV, et al. Structure of a type IV secretion system. Nature. 2014;508(7497):550–3. · DOI · Paper page
  32. Xiao J, Wang Q, Liu Q, Wang X, Liu H, Zhang Y. Isolation and identification of fish pathogen Edwardsiella tarda from mariculture in China. Aquac Res. 2008;40(1):13–7. · doi:10.1111/j.1365-2109.2008.02101.x
  33. Yu JE, Cho MY, Kim JW, Kang HY. Large antibiotic-resistance plasmid of Edwardsiella tarda contributes to virulence in fish. Microb Pathog. 2012;52(5):259–66. · doi:10.1016/j.micpath.2012.01.006
  34. Shao S, Lai Q, Liu Q, Wu H, Xiao J, Shao Z, Wang Q, Zhang Y. Phylogenomics characterization of a highly virulent Edwardsiella strain ET080813(T) encoding two distinct T3SS and three T6SS gene clusters: propose a novel species as Edwardsiella anguillarum sp. nov. Syst Appl Microbiol. 2015;38(1):36–47. · DOI · Paper page
  35. Zhao LJ, Lu JF, Nie P, Li AH, Xiong BX, Xie HX. Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri. Vet Microbiol. 2013;166(1–2):233–41. · DOI
  36. Fernandez DH, Pittman-Cooley L, Thune RL. Sequencing and analysis of the Edwardsiella ictaluri plasmids. Plasmid. 2001;45(1):52–6. · doi:10.1006/plas.2000.1499
  37. Welch TJ, Evenhuis J, White DG, McDermott PF, Harbottle H, Miller RA, Griffin M, Wise D. IncA/C plasmid-mediated florfenicol resistance in the catfish pathogen Edwardsiella ictaluri. Antimicrob Agents Chemother. 2009;53:845–6. · doi:10.1128/aac.01312-08
  38. Sun K, Wang H, Zhang M, Xiao Z, Sun L. Genetic mechanisms of multi-antimicrobial resistance in a pathogenic Edwardsiella tarda strain. Aquaculture. 2009;289(1):134–9. · DOI
  39. Nakamura Y, Takano T, Yasuike M, Sakai T, Matsuyama T, Sano M. Comparative genomics reveals that a fish pathogenic bacterium Edwardsiella tarda has acquired the locus of enterocyte effacement (LEE) through horizontal gene transfer. BMC Genomics. 2013;14:642. · doi:10.1186/1471-2164-14-642
  40. Xiao J, Chen T, Liu B, Yang W, Wang Q, Qu J, Zhang Y. Edwardsiella tarda mutant disrupted in type III secretion system and chorismic acid synthesis and cured of a plasmid as a live attenuated vaccine in turbot. Fish Shellfish Immunol. 2013;35(3):632–41. · doi:10.1016/j.fsi.2013.05.022
  41. den Hartigh AB, Rolan HG, de Jong MF, Tsolis RM. VirB3 to VirB6 and VirB8 to VirB11, but not VirB7, are essential for mediating persistence of Brucella in the reticuloendothelial system. J Bacteriol. 2008;190(13):4427–36. · doi:10.1128/jb.00406-08
  42. Nguyen KT, Piastro K, Derbyshire KM. LpqM, a mycobacterial lipoprotein-metalloproteinase, is required for conjugal DNA transfer in Mycobacterium smegmatis. J Bacteriol. 2009;191(8):2721–7. · DOI
  43. Wang JC. Cellular roles of DNA topoisomerases: a molecular perspective. Nat Rev Mol Cell Biol. 2002;3(6):430–40. · DOI · Paper page
  44. Meyer R. Identification of the mob genes of plasmid pSC101 and characterization of a hybrid pSC101-R1162 system for conjugal mobilization. J Bacteriol. 2000;182(17):4875–81. · doi:10.1128/jb.182.17.4875-4881.2000
  45. Farrand SK, Hwang I, Cook DM. The tra region of the nopaline-type Ti plasmid is a chimera with elements related to the transfer systems of RSF1010, RP4, and F. J Bacteriol. 1996;178(14):4233–47. · doi:10.1128/jb.178.14.4233-4247.1996
  46. Chakraborty S, Sivaraman J, Leung KY, Mok YK. Two-component PhoB-PhoR regulatory system and ferric uptake regulator sense phosphate and iron to control virulence genes in type III and VI secretion systems of Edwardsiella tarda. J Biol Chem. 2011;286(45):39417–30. · DOI
  47. Yin K, Wang Q, Xiao J, Zhang Y. Comparative proteomic analysis unravels a role for EsrB in the regulation of reactive oxygen species stress responses in Edwardsiella piscicida. FEMS Microbiol Lett. 2017;364(1):fnw269. · doi:10.1093/femsle/fnw269
  48. Andersson DI, Hughes D. Microbiological effects of sublethal levels of antibiotics. Nat Rev Microbiol. 2014;12(7):465–78. · DOI · Paper page
  49. Stevens AM, Shoemaker NB, Li LY, Salyers AA. Tetracycline regulation of genes on Bacteroides conjugative transposons. J Bacteriol. 1993;175(19):6134–41. · DOI
  50. Whittle G, Shoemaker NB, Salyers AA. Characterization of genes involved in modulation of conjugal transfer of the Bacteroides conjugative transposon CTnDOT. J Bacteriol. 2002;184(14):3839–47. · DOI
  51. Milton DL, Norqvist A, Wolf-Watz H. Cloning of a metalloprotease gene involved in the virulence mechanism of Vibrio anguillarum. J Bacteriol. 1992;174(22):7235–44. · doi:10.1128/jb.174.22.7235-7244.1992
  52. Wang SY, Lauritz J, Jass J, Milton DL. A ToxR homolog from Vibrio anguillarum serotype O1 regulates its own production, bile resistance, and biofilm formation. J Bacteriol. 2002;184(6):1630–9. · DOI
  53. Dennis JJ, Zylstra GJ. Plasposons: modular self-cloning minitransposon derivatives for rapid genetic analysis of gram-negative bacterial genomes. Appl Environ Microbiol. 1998;64(7):2710–5. · DOI · Paper page

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Metadata

Authors
Liu, Y., Gao, Y., Liu, X., Liu, Q., Zhang, Y., Wang, Q., & Xiao, J.
Venue
BMC Microbiology
Publisher
Springer Science and Business Media LLC
Published
2017-05-12
Volume
17
Issue
1
ISSN
1471-2180
DOI
10.1186/s12866-017-1013-7
Cited by
31
References
2
Language
en
Funders
  • National Natural Science Foundation of China (31430090, 31602200, 31672696)
  • Ministry of Agriculture of the People's Republic of China (CARS-50, nyhyzx-201303047)
  • Shanghai Pujiang Program (16PJD018)

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CoChat. (2026). Citation check: Transposon insertion sequencing reveals T4SS as the major genetic trait for conjugation transfer of multi-drug resistance pEIB202 from Edwardsiella. https://app.cochat.ai/papers/10.1186/s12866-017-1013-7
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Liu, Y., Gao, Y., Liu, X., Liu, Q., Zhang, Y., Wang, Q., & Xiao, J. (2017). Transposon insertion sequencing reveals T4SS as the major genetic trait for conjugation transfer of multi-drug resistance pEIB202 from Edwardsiella. BMC Microbiology. https://doi.org/10.1186/s12866-017-1013-7

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