Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis
Open accessAbstract
Background: Red algae Porphyra sensu lato grow naturally in the unfavorable intertidal environment, in which they are exposed to substantial temperature fluctuations. The strategies of Porphyra to tolerate cold stress are poorly understood. Results: Herein, investigations revealed that chilling and freezing induced alterations in the physiological properties, gene transcriptional profiles and metabolite levels in the economically important red algae species, Neoporphyra haitanensis. Control samples (kept at 20 °C) were compared to chilled thalli (10 and 4 °C) and to thalli under − 4 °C conditions. Chilling stress did not affect the health or photosynthetic efficiency of gametophytes, but freezing conditions resulted in the arrest of growth, death of some cells and a decrease in photosynthetic activity as calculated by Fv/Fm. Transcriptome sequencing analysis revealed that the photosynthetic system was down-regulated along with genes associated with carbon fixation and primary metabolic biosynthesis. Adaptive mechanisms included an increase in unsaturated fatty acids levels to improve membrane fluidity, an increase in floridoside and isofloridoside content to enhance osmotic resistance, and an elevation in levels of some resistance-associated phytohormones (abscisic acid, salicylic acid, and methyl jasmonic acid). These physiochemical alterations occurred together with the upregulation of ribosome biogenesis. Conclusions: N. haitanensis adopts multiple protective mechanisms to maintain homeostasis of cellular physiology in tolerance to cold stress.
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A decrease in the culture temperature leads to the shift of desaturases activity and an increase in the unsaturation level in fatty acids, which ensure the cell membrane fluidity under temperature stress conditions.
“Besides, a decrease in the culture temperature leads to the shift of desaturases activity and an increase in the unsaturation level in FAs, which ensure the cell membrane fluidity under temperature stress conditions [155–158].”
From Microalgae biofuels: illuminating the path to a sustainable future amidst challenges and opportunities (2024) · cites this paper as [156]
The Results section on fatty-acid composition reports increased unsaturation and changes in fatty-acid desaturase gene expression at lower culture temperatures in N. haitanensis. The Discussion says increased unsaturation could increase membrane fluidity, but the paper does not directly measure desaturase activity or establish that these changes ensure membrane fluidity. (LLM, conf=0.85)
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- Authors
- Zhu, S., Gu, D., Lu, C., Zhang, C., Chen, J., Yang, R., Luo, Q., Wang, T., Zhang, P., & Chen, H.
- Affiliations
- Ningbo University (CN)
- Zhejiang Mariculture Research Institute (CN)
- Corresponding author
- Chen, Haimin
- Venue
- BMC Plant Biology
- Published
- 2022-03-14
- Volume
- 22
- Issue
- 1
- Pages
- 114-114
- ISSN
- 1471-2229 (ISSN-L)
- PMC
- PMC8919617
- OpenAlex
- W4221028138
- Cited by
- 39
- References
- 55
- Citations by year
- 2026: 15 · 2025: 11 · 2024: 3 · 2023: 5 · 2022: 5
- FWCI
- 3.30
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- BMC Plant Biology (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Natural Science Foundation of China (2018YFD0900305, 2021Z103, 2021Z004, 2021C02069-9, 31872540, LY22C190002)
- Ningbo University
- K. C. Wong Magna Fund in Ningbo University
- Major Scientific and Technological Project of Zhejiang Province (2021C02069-9, 2021Z004, LY22C190002, 31872540, 2021Z103, 2018YFD0900305)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Physical Sciences › Environmental Science › Ecology › Physiological and biochemical adaptations
- Keywords
- Neoporphyra haitanensis, cold tolerance, freezing stress, chilling stress, intertidal red algae, Porphyra, photosynthetic efficiency, unsaturated fatty acids, membrane fluidity, floridoside, osmotic resistance, abscisic acid, salicylic acid, methyl jasmonic acid, plant hormones, ribosome biogenesis, CO2 fixation, gene expression
- MeSH terms
- Rhodophyta, Cold Temperature, Photosynthesis, Gene Expression Profiling, Cold-Shock Response
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