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Cited by 34Malaria Research and ControlInsect Resistance and GeneticsDengue and Mosquito Control Research

Anopheles coluzzii larval habitat and insecticide resistance in the island area of Manoka, Cameroon

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Etang, Josiane, Mbida Mbida, Arthur, Ntonga Akono, Patrick, Binyang, Jerome, Eboumbou Moukoko, Carole Else, Lehman, Leopold Gustave, Awono-Ambene, Parfait, Talipouo, Abdou +5 more·2016·BMC Infectious Diseases
DOI 10.1186/s12879-016-1542-yPMC PMC4875715
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Abstract

BACKGROUND: The effectiveness of Long-Lasting Insecticidal Nets and Indoor Residual Spraying in malaria vector control is threatened by vector resistance to insecticides. Knowledge of mosquito habitats and patterns of insecticide resistance would facilitate the development of appropriate vector control strategies. Therefore, we investigated An. coluzzii larval habitats and resistance to insecticides in the Manoka rural island area compared with the Youpwe suburban inland area, in Douala VI and II districts respectively. METHODS: Anopheline larvae and pupae were collected from open water bodies in December 2013 and April 2014 and reared until adult emergence. Two to four day old emerging females were morphologically identified as belonging to the An. gambiae complex and used for WHO susceptibility tests with 4 % DDT, 0.75 % permethrin, and 0.05 % deltamethrin, with or without piperonyl butoxide (PBO) synergist. Control and surviving specimens were identified down to the species using a PCR-RFLP method. Survivors were genotyped for kdr L1014 mutations using Hot Oligonucleotide Ligation Assay. RESULTS: In both study sites, ponds, residual puddles, boats, and drains were identified as the major An. gambiae s.l. larval habitats. A total of 1397 females, including 784 specimens from Manoka and 613 from Youpwe, were used for resistance testing. The two mosquito populations displayed resistance to DDT, permethrin and deltamethrin, with variable mortality rates from 1 % to 90 %. The knock-down times were also significantly increased (at least 2.8 fold). Pre-exposure of mosquitoes to PBO did not impact on their mortality to DDT, conversely the mortality rates to permethrin and deltamethrin were significantly increased (7.56 ≤ X(2) ≤ 48.63, df = 1, p < 0.01), suggesting involvement of P450 oxidases in pyrethroid resistance. A subsample of 400 An. gambiae s.l. specimens including 280 control and 120 survivors from bioassays were all found to be An. coluzzii species. Only the kdr 1014 F mutation was found in survivors, with 88.5 % (N = 76) and 75 % (N = 44) frequencies in Youpwe and Manoka respectively. CONCLUSION: This is the first report of An. coluzzii resistance to insecticides in an insular area in Cameroon. Since permanent larval habitats have been identified, larval source management strategies may be trialed in this area as complementary vector control interventions.

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  • Species diversity could be explained by the presence of different ecological factors such as permanent rivers, puddles, small dams, rice-growing areas and vegetable areas.

    “The species diversity could be explained by the presence of different ecologicals factors such as permanent rivers, puddles, small dams, rice-growing areas and vegetable areas.”

    From Resting and feeding behaviour of malaria vectors, in two areas of high long-lasting insecticidal nets coverage in Côte d'Ivoire (2024) · cites this paper as Etang et al. 2016

    The Results section on larval habitats reports An. coluzzii larvae near the Wouri River and in residual puddles, supporting part of the habitat list. It does not identify small dams, rice-growing areas or vegetable-growing areas as habitats, or explain species diversity by their presence. (LLM, conf=0.84)

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Authors
Etang, J., Mbida Mbida, A., Ntonga Akono, P., Binyang, J., Eboumbou Moukoko, C. E., Lehman, L. G., Awono-Ambene, P., Talipouo, A., Ekoko Eyisab, W., Tagne, D., Tchoffo, R., Manga, L., & Mimpfoundi, R.
Affiliations
  • University of Douala (CM)
  • World Health Organization Regional Office for Africa (CG)
Corresponding author
Etang, Josiane
ORCID
Venue
BMC Infectious Diseases
Publisher
Springer Science and Business Media LLC
Published
2016-05-20
Volume
16
Issue
1
Pages
217-217
ISSN
1471-2334 (ISSN-L)
DOI
10.1186/s12879-016-1542-y
PMC
PMC4875715
OpenAlex
W2396318033
Cited by
34
References
2
Citations by year
2026: 2 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 8 · 2021: 6 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 1
FWCI
2.64
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • BMC Infectious Diseases (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Agence Universitaire Francophone (not applicable)
  • PARTEC Afrique Central Douala Cameroon (not applicable)
  • International Society for Health Research and Training (ISRT-Health) (not applicable)
  • World Health Organization (CH)
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
  • Social Sciences › Sociology and Political Science › Dengue and Mosquito Control Research
Keywords
Anopheles coluzzii, larval habitat, insecticide resistance, Anopheles gambiae s.l., pyrethroid resistance, deltamethrin, permethrin, DDT, piperonyl butoxide, CYP
MeSH terms
Animals, Anopheles, Cameroon, Female, Gene Frequency, Genotype, Insecticide Resistance, Insecticides, Larva, Mutation, Nitriles, Pupa, Pyrethrins, Polymorphism, Restriction Fragment Length, Ecosystem, Permethrin, Islands

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