Cited by 120Circular RNAs in diseasesTreatment of Major DepressionRNA regulation and disease

Abnormal expression of rno_circRNA_014900 and rno_circRNA_005442 induced by ketamine in the rat hippocampus

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Mao, Jing, Li, Tianmei, Fan, Di, Zhou, Hongli, Feng, Jianguo, Liu, Li, Zhang, Chunxiang, Wang, Xiaobin·2020·BMC Psychiatry

Abstract

BACKGROUND: Recent studies have shown that circular RNA (circRNA) is rich in microRNA (miRNA) binding sites. We have previously demonstrated that the antidepressant effect of ketamine is related to the abnormal expression of various miRNAs in the brain. This study determined the expression profile of circRNAs in the hippocampus of rats treated with ketamine. METHODS: The aberrantly expressed circRNAs in rat hippocampus after ketamine injection were analyzed by microarray chip, and we further validated these circRNAs by quantitative reverse-transcription PCR (qRT-PCR). The target genes of the different circRNAs were predicted using bioinformatic analyses, and the functions and signal pathways of these target genes were investigated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. RESULTS: Microarray analysis showed that five circRNAs were aberrantly expressed in rat hippocampus after ketamine injection (fold change > 2.0, p < 0.05). The results from the qRT-PCR showed that one of the circRNAs was significantly increased (rno_circRNA_014900; fold change = 2.37; p = 0.03), while one was significantly reduced (rno_circRNA_005442; fold change = 0.37; p = 0.01). We discovered a significant enrichment in several GO terms and pathways associated with depression. CONCLUSION: Our findings showed the abnormal expression of ketamine-induced hippocampal circRNAs in rats.

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Authors
Mao, J., Li, T., Fan, D., Zhou, H., Feng, J., Liu, L., Zhang, C., & Wang, X.
Affiliations
  • Southwest Medical University (CN)
  • Affiliated Hospital of Southwest Medical University (CN)
  • University of Alabama at Birmingham (US)
Corresponding author
Wang, Xiaobin
Venue
BMC Psychiatry
Published
2020-01-02
Volume
20
Issue
1
Pages
1-1
ISSN
1471-244X (ISSN-L)
OpenAlex
W3002516758
Cited by
120
References
34
Citations by year
2026: 1 · 2025: 27 · 2024: 23 · 2023: 14 · 2022: 18 · 2021: 33 · 2020: 3
FWCI
5.51
Type
journal article
Language
en
Open access
Locations
  • BMC Psychiatry (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Department of Science &Technology of Sichuan Province (14JC0093)
  • the Projects of the National Natural Science Foundation of China (81271478)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Circular RNAs in diseases
  • Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
Keywords
circular RNA, ketamine, hippocampus, depression, antidepressant effects, microRNA binding sites, circRNA expression profiling, rat model
MeSH terms
RNA, Circular, Animals, Hippocampus, Ketamine, Male, Signal Transduction, Rats, Sprague-Dawley, Excitatory Amino Acid Antagonists, Computational Biology, Gene Expression Profiling, Rats, Real-Time Polymerase Chain Reaction

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