MicroRNA expression profile and functional analysis reveal that miR-206 is a critical novel gene for the expression of BDNF induced by ketamine.
Abstract
Depression is a major social and health concern, and ketamine exerts a quick, remarkable and persistent anti-depressive effect. microRNAs (miRNAs) show remarkable potential in the treatment of clinical depression. Here, we determined the expression profile of miRNAs in the hippocampus of rats treated with ketamine (15 mg/kg). The results suggest that multiple miRNAs were aberrantly expressed in rat hippocampus after ketamine injection (18 miRNAs were significantly reduced, while 22 miRNAs were significantly increased). Among them, miR-206 was down-regulated in ketamine-treated rats. In both cultured neuronal cells in vitro and hippocampus in vivo, we identified that the brain-derived neurotrophic factor (BDNF) was a direct target gene of miR-206. Via this target gene, miR-206 strongly modulated the expression of BDNF. Moreover, overexpression of miR-206 significantly attenuated ketamine-induced up-regulation of BDNF. The results indicated that miRNA-206 was involved in novel therapeutic targets for the anti-depressive effect of ketamine.
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Ketamine downregulated 18 miRNAs levels and upregulated 22 miRNAs levels in rats.
“Ketamine downregulated 18 miRNAs levels and upregulated 22 miRNAs levels in rats.344”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [344]
miRNA-206 may moderate ketamine's antidepressant effect.
“Thus, miRNA-206 may moderate ketamine’s antidepressant effect.”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [344]
MiR-206 suppressed ketamine's ability to up-regulate BDNF expression and is downregulated by ketamine.
“MiR-206 suppressed ketamine’s ability to up-graduate BDNF expression and is downregulated by ketamine.344”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [344]
MiRNAs may govern cell proliferation, division, apoptosis, and metabolism as regulators of numerous biological processes by promoting degradation of mRNAs and inhibiting their translation, so that targeted mRNAs play a negative feedback regulatory function.
“It may govern cell proliferation, division, apoptosis, and metabolism as regulators of numerous biological processes by promoting the degradation of mRNAs and inhibiting their translation, so that its targeted mRNAs play a negative feedback regulatory function [11].”
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- Authors
- Yang, X., Yang, Q., Wang, X., Luo, C., Wan, Y., Li, J., Liu, K., Zhou, M., & Zhang, C.
- Affiliations
- Southwest Medical University (CN)
- University of Medicine and Dentistry of New Jersey (US)
- Rush University (US)
- Corresponding author
- Wang, Xiaobin
- Venue
- NeuroMolecular Medicine
- Published
- 2014-05-18
- Volume
- 16
- Issue
- 3
- Pages
- 594-605
- ISSN
- 1535-1084 (ISSN-L)
- 1559-1174
- OpenAlex
- W2043756937
- Cited by
- 69
- References
- 55
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 8 · 2023: 2 · 2022: 6 · 2021: 7 · 2020: 6 · 2019: 4 · 2018: 5 · 2017: 6 · 2016: 8 · 2015: 8 · 2014: 1
- FWCI
- 5.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- NeuroMolecular Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Treatment of Major Depression
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Keywords
- miR-206, microRNA expression profiles, ketamine, depression, antidepressant effects, brain-derived neurotrophic factor, hippocampus, microRNA therapeutic target
- MeSH terms
- Anesthetics, Dissociative, Animals, Cells, Cultured, Gene Expression Regulation, Hippocampus, Ketamine, Male, Neurons, Oligodeoxyribonucleotides, Random Allocation, Transfection, Rats, Sprague-Dawley, Apoptosis, Antidepressive Agents, Second-Generation, Excitatory Amino Acid Antagonists, Brain-Derived Neurotrophic Factor, Gene Expression Profiling, MicroRNAs, Rats, Gene Knockdown Techniques
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