Oleaginous yeasts- substrate preference and lipid productivity: a view on the performance of microbial lipid producers
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BACKGROUND: Oleaginous yeasts are promising microbial platforms for sustainable, bio-based production of biofuels and oleochemical building blocks. Bio-based residues provide sustainable and cost-effective carbon sources for fermentative yeast oil production without land-use change. Considering the regional abundancy of different waste streams, we chose complex biomass residue streams of marine origin; macroalgae hydrolysate, and terrestrial origin; wheat straw hydrolysate in the presence, and absence of corn steep liquor as a complex nitrogen source. We investigated the biomass and lipid yields of an array of well-described oleaginous yeasts; R. glutinis, T. asahii, R. mucilaginosa, R. toruloides, C. oleaginosus growing on these hydrolysates. Furthermore, their sugar utilization, fatty acid profile, and inhibitory effect of the hydrolysates on yeast growth were compared. For correlative reference, we initially performed comparative growth experiments for the strains on individual monomeric sugars separately. Each of these monomeric sugars was a dominant carbon source in the complex biomass hydrolysates evaluated in this study. In addition, we evaluated N-acetylglucosamine, the monomeric building block of chitin, as a low-cost nitrogen and carbon source in yeast fermentation. RESULTS: C. oleaginosus provided the highest biomass and lipid yields. In the wheat straw and brown algae hydrolysates, this yeast strain gained 7.5 g/L and 3.8 g/L lipids, respectively. Cultivation in algae hydrolysate resulted in a higher level of unsaturated fatty acids in the lipids accumulated by all yeast strains. R. toruloides and C. oleaginosus were able to effectively co-utilize mannitol, glucose, and xylose. Growth rates on wheat straw hydrolysate were enhanced in presence of corn steep liquor. CONCLUSIONS: Among the yeast strains investigated in this study, C. oleaginosus proved to be the most versatile strain in terms of substrate utilization, productivity, and tolerance in the complex media. Various fatty acid profiles obtained on each substrate encourage the manipulation of culture conditions to achieve the desired fatty acid composition for each application. This could be accomplished by combining the element of carbon source with other formerly studied factors such as temperature and oxygen. Moreover, corn steep liquor showed promise for enhancement of growth in the oleaginous strains provided that carbon substrate is available.
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Cutaneotrichosporon oleaginosus yeast is capable of producing up to 90% (w/w) lipids per biomass in a fermentation process, and can be grown on residue streams.
“the production of second-generation biodiesel is possible from microbial lipids produced by organisms, such as Cutaneotrichosporon oleaginosus, a yeast capable of producing up to 90% (w/w) lipids per biomass in a fermentation process, which can be grown on residue streams (e.g., wheat bran hydrolysate medium) [46–49].”
From The potential of biofuels from first to fourth generation (2023) · cites this paper as [47]
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The potential of biofuels from first to fourth generation
Cavelius, P., Engelhart-Straub, S., Mehlmer, N., Lercher, J., Awad, D., & Brück, T. (2023) · PLOS Biology · doi:10.1371/journal.pbio.3002063 · Cited by 224 · see citing works
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- Authors
- Shaigani, P., Awad, D., Redai, V., Fuchs, M., Haack, M., Mehlmer, N., & Brueck, T.
- Affiliations
- Technical University of Munich (DE)
- Corresponding author
- Mehlmer, Norbert
- Brueck, Thomas
- Venue
- Microbial Cell Factories
- Published
- 2021-12-07
- Volume
- 20
- Issue
- 1
- Pages
- 220-220
- ISSN
- 1475-2859 (ISSN-L)
- PMC
- PMC8650408
- OpenAlex
- W4200096234
- Cited by
- 68
- References
- 69
- Citations by year
- 2026: 13 · 2025: 14 · 2024: 14 · 2023: 18 · 2022: 9
- FWCI
- 3.45
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Microbial Cell Factories (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- mediaTUM (Technical University of Munich) (submittedVersion, other-oa)
- mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion, other-oa)
- Funders
- Bundesministerium für Bildung und Forschung (033RC012B)
- Technische Universität München
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- oleaginous yeast, yeast lipid production, substrate utilization, wheat straw hydrolysate, corn steep liquor, nitrogen source, Rhodotorula glutinis, Rhodotorula toruloides, Trichosporon asahii, Rhodotorula mucilaginosa, fatty acid composition, unsaturated fatty acids, mannitol, xylose, N-acetylglucosamine, chitin, biofuels, oleochemicals
- MeSH terms
- Basidiomycota, Carbon, Fermentation, Lipids, Nitrogen, Rhodotorula, Yeasts, Biomass, Lipid Metabolism, Biofuels
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