Efficient conversion of acetate into lipids by the oleaginous yeast Cryptococcus curvatus
Open accessAbstract
BACKGROUND: Acetic acid is routinely generated during lignocelluloses degradation, syngas fermentation, dark hydrogen fermentation and other anaerobic bioprocesses. Acetate stream is commonly regarded as a by-product and detrimental to microbial cell growth. Conversion of acetate into lipids by oleaginous yeasts may be a good choice to turn the by-product into treasure. RESULTS: Ten well-known oleaginous yeasts were evaluated for lipid production on acetate under flask culture conditions. It was found that all of those yeasts could use acetate for microbial lipid production. In particular, Cryptococcus curvatus accumulated lipids up to 73.4 % of its dry cell mass weight. When the culture was held in a 3-L stirred-tank bioreactor, cell mass, lipid content, lipid yield and acetate consumption rate were 8.1 g/L, 49.9 %, 0.15 g/g and 0.64 g/L/h, respectively. The fatty acid compositional profiles of the acetate-derived lipids were similar to those of vegetable oil, suggesting their potential for biodiesel production. Continuous cultivation of C. curvatus was conducted under nitrogen-rich condition at a dilution rate of 0.04 h(-1), the maximal lipid content and lipid yield were 56.7 % and 0.18 g/g, respectively. The specific lipid formation rate, lipid content and lipid yield were all higher under nitrogen-rich conditions than those obtained under nitrogen-limited conditions at the same dilution rates. Effective lipid production by C. curvatus was observed on corn stover hydrolysates containing 15.9 g/L acetate. CONCLUSIONS: Acetate is an effective carbon source for microbial lipid production by oleaginous yeasts. Continuous cultivation of C. curvatus on acetate was promising for lipid production under both nitrogen-rich and nitrogen-limited conditions. These results provide valuable information for developing and designing more efficient acetate-into-lipids bioprocess.
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Gong et al. demonstrated lipid synthesis from acetate by multiple oleaginous yeast strains, with Cryptococcus curvatus ATCC 20509 yielding a lipid concentration of 4.2 g/L and 73.4% of the cell dry weight.
“For instance, Gong et al. demonstrated lipid synthesis from acetate by multiple oleaginous yeast strains with one, Cryptococcus curvatus ATCC 20509, yielding a lipid concentration of 4.2 g/L and 73.4% of the cell dry weight (Gong et al., 2015).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Gong et al., 2015
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Cites
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Miller, G. L. (1959) · Analytical Chemistry · doi:10.1021/ac60147a030 · Cited by 28,963 · see citing works
Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition
Palmqvist, E. A., & Hahn‐Hägerdal, B. (2000) · Bioresource Technology · doi:10.1016/s0960-8524(99)00161-3 · Cited by 2,518 · see citing works
The Biochemistry and Molecular Biology of Lipid Accumulation in Oleaginous Microorganisms
Ratledge, C., & Wynn, J. P. (2002) · Advances in applied microbiology · doi:10.1016/s0065-2164(02)51000-5 · Cited by 1,027 · see citing works
High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture
Li, Y., Zhao, Z. K., & Bai, F. (2007) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2007.02.008 · Cited by 698 · see citing works
Self-citationLipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production
Papanikolaou, S., & Aggelis, G. (2011) · European Journal of Lipid Science and Technology · doi:10.1002/ejlt.201100014 · Cited by 683 · see citing works
Papanikolaou, S., & Aggelis, G. (2002) · Bioresource Technology · doi:10.1016/s0960-8524(01)00149-3 · Cited by 478 · see citing works
Yu, X., Zheng, Y., Dorgan, K. M., & Chen, S. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.02.081 · Cited by 451 · see citing works
Microbiology of synthesis gas fermentation for biofuel production
Henstra, A. M., Sipma, J., Rinzema, A., & Stams, A. J. M. (2007) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2007.03.008 · Cited by 449 · see citing works
Bioconversion of volatile fatty acids into lipids by the oleaginous yeast Yarrowia lipolytica
Fontanille, P., Kumar, V., Christophe, G., Nouaille, R., & Larroche, C. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.02.091 · Cited by 327 · see citing works
Fei, Q., Chang, H. N., Shang, L., Choi, J., Kim, N., & Kang, J. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.10.141 · Cited by 317 · see citing works
Microbial lipid-based lignocellulosic biorefinery: feasibility and challenges
Jin, M., Slininger, P. J., Dien, B. S., Waghmode, S., Moser, B. R., Orjuela, A. D. P., Sousa, L. D. C., & Balan, V. (2014) · Trends in biotechnology · doi:10.1016/j.tibtech.2014.11.005 · Cited by 315 · see citing works
Effects of biomass hydrolysis by-products on oleaginous yeast Rhodosporidium toruloides
Hu, C., Zhao, X., Zhao, J. F., et al. (2009) · Bioresource Technology · doi:10.1016/j.biortech.2009.04.041 · Cited by 311 · see citing works
Huang, C., Chen, X., Xiong, L., Chen, X.-D., Ma, L., & Chen, Y. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.08.010 · Cited by 305 · see citing works
Biodiesel production by direct methanolysis of oleaginous microbial biomass
Liu, B., & Zhao, Z. K. (2007) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.1744 · Cited by 301 · see citing works
Self-citationPhosphate-limitation mediated lipid production by Rhodosporidium toruloides
Wu, S., Hu, C., Jin, G., Zhao, X., & Zhao, Z. K. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.02.111 · Cited by 280 · see citing works
Self-citationEnhanced biofuel production through coupled acetic acid and xylose consumption by engineered yeast
Wei, N., Quarterman, J., Kim, S. R., Cate, J. H. D., & Jin, Y. (2013) · Nature Communications · doi:10.1038/ncomms3580 · Cited by 243 · see citing works
Younesi, H., Najafpour, G. D., & Mohamed, A. R. (2005) · Biochemical Engineering Journal · doi:10.1016/j.bej.2005.08.015 · Cited by 238 · see citing works
Screening of Oleaginous Yeast Strains Tolerant to Lignocellulose Degradation Compounds
Chen, X., Li, Z., Zhang, X., et al. (2009) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-008-8491-x · Cited by 238 · see citing works
Single cell oil production from rice hulls hydrolysate
Economou, C. Ν., Aggelis, G., Pavlou, S., & Vayenas, D. V. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.08.025 · Cited by 214 · see citing works
Co-fermentation of cellobiose and xylose by Lipomyces starkeyi for lipid production
Gong, Z., Wang, Q., Shen, H., et al. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.04.063 · Cited by 171 · see citing works
Self-citationStraub, M., Demler, M., Weuster-Botz, D., & Dürre, P. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.03.005 · Cited by 162 · see citing works
Evans, C. T., & Ratledge, C. (1983) · Lipids · doi:10.1007/bf02534673 · Cited by 139 · see citing works
Chi, Z., Zheng, Y., Ma, J., & Chen, S. (2011) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2011.04.124 · Cited by 134 · see citing works
Yeast fermentation of carboxylic acids obtained from pyrolytic aqueous phases for lipid production
Lian, J., Garcı̀a-Pèrez, M., Coates, R., et al. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.05.010 · Cited by 122 · see citing works
Production of Oils from Acetic Acid by the Oleaginous Yeast Cryptococcus curvatus
Christophe, G., Deo, J. L., Kumar, V., Nouaille, R., Fontanille, P., & Larroche, C. (2011) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-011-9507-5 · Cited by 107 · see citing works
Huang, C., Wu, H., Liu, Z.-J., Cai, J., Lou, W., & Zong, M. (2012) · Biotechnology for Biofuels · doi:10.1186/1754-6834-5-4 · Cited by 102 · see citing works
Oleaginous yeast Cryptococcus curvatus for biofuel production: Ammonia’s effect
Zheng, Y., Chi, Z., Ahring, B. K., & Chen, S. (2012) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2011.12.022 · Cited by 92 · see citing works
Xu, X., Kim, J. Y., Cho, H. U., Park, H. R., & Park, J. M. (2014) · Chemical Engineering Journal · doi:10.1016/j.cej.2014.12.011 · Cited by 85 · see citing works
Lipid accumulation in Schizochytrium G13/2S produced in continuous culture
Ganuza, E., & Izquierdo, M. S. (2007) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-007-1019-4 · Cited by 85 · see citing works
Zhang, G., French, W. T., Hernandez, R. A., et al. (2010) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2010.10.009 · Cited by 84 · see citing works
Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides
Shen, H., Gong, Z., Yang, X., et al. (2013) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2013.08.010 · Cited by 79 · see citing works
Self-citationBéligon, V., Poughon, L., Christophe, G., et al. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.06.041 · Cited by 77 · see citing works
Mathematical modelling of lipid production by oleaginous yeasts in continuous cultures
Ykema, A., Verbree, E. C., van Verseveld, H. W., et al. (1986) · Antonie van Leeuwenhoek · doi:10.1007/bf00423410 · Cited by 77 · see citing works
Kabel, M. A., Yeoman, C. J., Han, Y., et al. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.05321-11 · Cited by 75 · see citing works
Lipid production from corn stover by the oleaginous yeast Cryptococcus curvatus
Gong, Z., Shen, H., Yang, X., Wang, Q., Xie, H., & Zhao, Z. K. (2014) · Biotechnology for Biofuels · doi:10.1186/s13068-014-0158-y · Cited by 70 · see citing works
Self-citationRuan, Z., Hollinshead, W. D., Isaguirre, C. N., et al. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.02.026 · Cited by 66 · see citing works
Hall, M. J., & Ratledge, C. (1977) · Applied and Environmental Microbiology · doi:10.1128/aem.33.3.577-584.1977 · Cited by 60 · see citing works
Granger, L.-M., Perlot, P., Goma, G., & Pareilleux, A. (1993) · Biotechnology and Bioengineering · doi:10.1002/bit.260421004 · Cited by 42 · see citing works
Lipid Formation in Cryptococcus terricolus. I. Nitrogen Nutrition and Lipid Formation.
Pedersen, T. A., Horning, E. C., Rönnquist, A., et al. (1961) · Acta chemica Scandinavica/Acta chemica Scandinavica. B, Organic chemistry and biochemistry/Acta chemica Scandinavica. A, Physical and inorganic chemistry/Acta chemica Scandinavica. Series B. Organic chemistry and biochemistry/Acta chemica Scandinavica. Series A, Physical and inorganic chemistry · doi:10.3891/acta.chem.scand.15-0651 · Cited by 39 · see citing works
A relationship between growth and lipid accumulation in Candida curvata D
Brown, B. D., HSU, K. H., Hammond, E. G., et al. (1989) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(89)90010-x · Cited by 27 · see citing works
Production of microbial lipids: III. Influence of C/N ratio—experimental observations
Sattur, A. P., & Karanth, N. G. (1989) · Biotechnology and Bioengineering · doi:10.1002/bit.260340618 · Cited by 11 · see citing works
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- Authors
- Gong, Z., Shen, H., Zhou, W., Wang, Y., Yang, X., & Zhao, Z. K.
- Affiliations
- Dalian Institute of Chemical Physics (CN)
- Wuhan University of Science and Technology (CN)
- Corresponding author
- Gong, Zhiwei
- Zhao, Zongbao K.
- Venue
- Biotechnology for Biofuels
- Published
- 2015-11-25
- Volume
- 8
- Issue
- 1
- Pages
- 189-189
- ISSN
- 1754-6834 (ISSN-L)
- PMC
- PMC4658814
- OpenAlex
- W2180959746
- Cited by
- 141
- References
- 41
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 12 · 2023: 9 · 2022: 18 · 2021: 16 · 2020: 19 · 2019: 16 · 2018: 12 · 2017: 14 · 2016: 8
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Biotechnology for Biofuels (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Dalian Institute of Chemical Physics, CAS Institutional Repository (submittedVersion)
- Funders
- National Natural Science Foundation of China (21325627)
- National Basic Research and Development Program of China (2011CB707405)
- Young Scientists Foundation of Wuhan University of Science and Technology (2015XZ013)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- acetate conversion, microbial lipid production, oleaginous yeast, Cryptococcus curvatus, biodiesel feedstock, fatty acid composition, continuous cropping, corn stover hydrolysate, lignocellulosic biorefinery, by-product utilization
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