CoChat
Sign Up
Cited by 58Ruminant Nutrition and Digestive PhysiologyGut microbiota and healthAnimal Nutrition and Physiology

Sex differences in rumen fermentation and microbiota of Tibetan goat

Open access
Guo, Xinyu, Sha, Yuzhu, Lv, Weibing, Pu, Xiaoning, Liu, Xiu, Luo, Yuzhu, Hu, Jiang, Wang, Jiqing +2 more·2022·Microbial Cell Factories
DOI 10.1186/s12934-022-01783-8PMC PMC8991483

Published version of a preprint

Open this paper in CoChat — free accountOpen PDF

Abstract

Background: The gut microbiota play an important role in maintaining host metabolism, the immune system and health, while sex, genotype, diet and health have specific effects on the composition of the gut microbiota. Therefore, to explore the sex differences in the structure and function of rumen microbiota in Tibetan goats, herein we analyzed sex differences in rumen fermentation parameters, rumen microbiota and the expression of genes related to VFA transport in Tibetan goats. Results: The results showed that the contents of acetic acid and propionic acid in the rumen of TGM (Tibetan goat male) were significantly higher than those in TGFm (Tibetan goat female) ( P < 0.05), and total VFAs was significantly higher in TGM than TGFm ( P < 0.05). Expression of the VFA transport-related genes DRA , AE2 , MCT-1 , NHE1 , and NHE2 in the rumen epithelium of TGFm was significantly higher than that in TGM. Analysis of the composition and structure of the rumen microbiota revealed significant sex differences. At the phylum level, Firmicutes and Bacteroidetes were the dominant phyla in Tibetan goats. In addition, Fibrobacteres and Spirochaetes had significantly greater relative abundances in TGFm than in TGM ( P < 0.05). At the genus level, the relative abundance of Fibrobacter , Ruminococcus_1 and Pyramidobacter was significantly higher in TGFm than in TGM ( P < 0.05). The functional prediction results showed that replication, recombination and repair, RNA processing and modification were mainly enriched in TGFm ( P < 0.05). Conclusions: Correlation analysis revealed significant associations of some rumen microbiota with the fermentation product VFAs and VFA transport-related genes. We concluded that yearling TGM and TGFm have distinct fermentation and metabolism abilities when adapting to the plateau environment, which provides a certain sex reference basis for Tibetan goat adaptation to the plateau environment.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1186/s12934-022-01783-8

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The molecular and cellular mechanisms underlying sex differences in Alzheimer's disease pathogenesis remain poorly understood.

    “Despite the continuous effort in the field, the molecular and cellular mechanisms underlying sex differences in AD pathogenesis remain poorly understood [9].”

    From Sex specific molecular networks and key drivers of Alzheimer’s disease (2023) · cites this paper as [9]

    The Introduction and Abstract Background discuss sex differences in Tibetan goat rumen microbiota, fermentation, and gene expression, not Alzheimer’s disease. Searches for Alzheimer’s disease, AD pathogenesis, dementia, and neurodegeneration found no passage addressing whether its sex-specific molecular or cellular mechanisms are poorly understood. (LLM, conf=0.78)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 41

  1. [1]Jin M Lu J Fei X Lu Z Quan K Liu Y Chu M Di R Wei C Wang H Selection signatures analysis reveals genes associated with high-altitude adaptation in Tibetan goats from Nagqu Tibet Animals (Basel) 2020 10 9 1599 10.3390/ani10091599 PMC7552128 32911823 · DOI
  2. [2]Thompson LG Yao T Mosley-Thompson E Davis ME Henderson KA Lin P A high-resolution millennial record of the south Asian monsoon from himalayan ice cores Science 2000 289 5486 1916 1920 10.1126/science.289.5486.1916 10988068 · DOI
  3. [3]Deng J Feng J Li L Zhong T Wang L Guo J Ba G Song T Zhang H Polymorphisms, differentiation, and phylogeny of 10 Tibetan goat populations inferred from mitochondrial D-loop sequences Mitochondrial DNA A DNA Mapp Seq Anal 2018 29 3 439 445 10.1080/24701394.2017 28358643 · DOI
  4. [4]Guo J Tao H Li P Li L Zhong T Wang L Ma J Chen X Song T Zhang H Whole-genome sequencing reveals selection signatures associated with important traits in six goat breeds Sci Rep 2018 8 1 10405 10.1038/s41598-018-28719-w 29991772 PMC6039503 · DOI
  5. [5]Liu X Sha Y Dingkao R Zhang W Lv W Wei H Shi H Hu J Wang J Li S Hao Z Luo Y Interactions between rumen microbes, VFAs, and host genes regulate nutrient absorption and epithelial barrier function during cold season nutritional stress in Tibetan sheep Front Microbiol 2020 11 593062 10.3389/fmicb.2020.593062 33250882 PMC7674685 · DOI
  6. [6]Zhang Z Xu D Wang L Hao J Wang J Zhou X Wang W Qiu Q Huang X Zhou J Long R Zhao F Shi P Convergent evolution of rumen microbiomes in high-altitude mammals Curr Biol 2016 26 14 1873 1879 10.1016/j.cub.2016.05.012 27321997 · DOI
  7. [7]Liu C Wu H Liu S Chai S Meng Q Zhou Z Dynamic Alterations in Yak Rumen bacteria community and metabolome characteristics in response to feed type Front Microbiol 2019 10 1116 10.3389/fmicb.2019.01116 31191470 PMC6538947 · DOI
  8. [8]Guo H Zhou G Tian G Liu Y Dong N Li L Zhang S Chai H Chen Y Yang Y Changes in Rumen microbiota affect metabolites, immune responses and antioxidant enzyme activities of sheep under cold stimulation Animals 2021 11 3 712 10.3390/ani11030712 33807979 PMC7999998 · DOI
  9. [9]Zhong S Ding Y Wang Y Zhou G Guo H Chen Y Yang Y Temperature and humidity index (THI)-induced rumen bacterial community changes in goats Appl Microbiol Biotechnol 2019 103 7 3193 3203 10.1007/s00253-019-09673-7 30793235 · DOI
  10. [10]Osadchiy V Martin CR Mayer EA The gut-brain axis and the microbiome: mechanisms and clinical implications Clin Gastroenterol Hepatol 2019 17 2 322 332 10.1016/j.cgh.2018.10.002 30292888 PMC6999848 · DOI · Paper page
  11. [11]Chaloner A Greenwood-Van MB Sexually dimorphic effects of unpredictable early life adversity on visceral pain behavior in a rodent model J Pain 2013 14 3 270 280 10.1016/j.jpain.2012.11.008 23348370 · DOI
  12. [12]Sinha T Vich Vila A Garmaeva S Jankipersadsing SA Imhann F Collij V Bonder MJ Jiang X Gurry T Alm EJ D'Amato M Weersma RK Scherjon S Wijmenga C Fu J Kurilshikov A Zhernakova A Analysis of 1135 gut metagenomes identifies sex-specific resistome profiles Gut Microbes 2019 10 3 358 366 10.1080/19490976.2018.1528822 30373468 PMC6546312 · DOI · Paper page
  13. [13]Elderman M Hugenholtz F Belzer C Boekschoten M van Beek A de Haan B Savelkoul H de Vos P Faas M Sex and strain dependent differences in mucosal immunology and microbiota composition in mice Biol Sex Differ 2018 9 1 26 10.1186/s13293-018-0186-6 29914546 PMC6006852 · DOI
  14. [14]Markle JG Frank DN Mortin-Toth S Robertson CE Feazel LM Rolle-Kampczyk U von Bergen M McCoy KD Macpherson AJ Danska JS Sex differences in the gut microbiome drive hormone-dependent regulation of autoimmunity Science 2013 339 6123 1084 1088 10.1126/science.1233521 23328391 · DOI · Paper page
  15. [16]Bhat AH Khan I Usmani MA Umapathi R Al-Kindy SMZ Cellulose an ageless renewable green nanomaterial for medical applications: an overview of ionic liquids in extraction, separation and dissolution of cellulose Int J Biol Macromol 2019 129 750 777 10.1016/j.ijbiomac.2018.12.190 30593803 · DOI
  16. [17]Russell JB Rychlik JL Factors that alter rumen microbial ecology Science 2001 292 5519 1119 1122 10.1126/science.1058830 11352069 · DOI
  17. [18]Connor EE Li RW Baldwin RL Li C Gene expression in the digestive tissues of ruminants and their relationships with feeding and digestive processes Animal 2010 4 7 993 1007 10.1017/S1751731109991285 22444605 · DOI
  18. [19]Dominianni C Sinha R Goedert JJ Pei Z Yang L Hayes RB Ahn J Sex, body mass index, and dietary fiber intake influence the human gut microbiome PLoS ONE. 2015 10 4 e0124599 10.1371/journal.pone.0124599 25874569 PMC4398427 · DOI · Paper page
  19. [20]Fernandes KA Kittelmann S Rogers CW Gee EK Bolwell CF Bermingham EN Thomas DG Faecal microbiota of forage-fed horses in New Zealand and the population dynamics of microbial communities following dietary change PLoS ONE 2014 9 11 112846 10.1371/journal.pone.0112846 PMC4226576 25383707 · DOI · Paper page
  20. [22]Thoetkiattikul H Mhuantong W Laothanachareon T Tangphatsornruang S Pattarajinda V Eurwilaichitr L Champreda V Comparative analysis of microbial profiles in cow rumen fed with different dietary fiber by tagged 16S rRNA gene pyrosequencing Curr Microbiol 2013 67 2 130 137 10.1007/s00284-013-0336-3 23471692 · DOI
  21. [23]Spence C Wells WG Smith CJ Characterization of the primary starch utilization operon in the obligate anaerobe Bacteroides fragilis: Regulation by carbon source and oxygen J Bacteriol 2006 188 13 4663 4672 10.1128/JB.00125-06 16788175 PMC1482989 · DOI
  22. [24]Wang X Zhang Y Wen Q Wang Y Wang Z Tan Z Wu K Sex differences in intestinal microbial composition and function of Hainan special wild boar Animals (Basel) 2020 10 9 1553 10.3390/ani10091553 PMC7552319 32887230 · DOI
  23. [25]Thomas F Hehemann JH Rebuffet E Czjzek M Michel G Environmental and gut bacteroidetes: the food connection Front Microbiol 2011 2 93 10.3389/fmicb.2011.00093 21747801 PMC3129010 · DOI
  24. [26]Magrone T Jirillo E The interplay between the gut immune system and microbiota in health and disease: nutraceutical intervention for restoring intestinal homeostasis Curr Pharm Des 2013 19 7 1329 1342 10.2174/138161213804805793 23151182 · DOI
  25. [27]Kaakoush NO Insights into the role of erysipelotrichaceae in the human host Front Cell Infect Microbiol 2015 5 84 10.3389/fcimb.2015.00084 26636046 PMC4653637 · DOI
  26. [28]Hess M Sczyrba A Egan R Kim TW Chokhawala H Schroth G Luo S Clark DS Chen F Zhang T Mackie RI Pennacchio LA Tringe SG Visel A Woyke T Wang Z Rubin EM Metagenomic discovery of biomass-degrading genes and genomes from cow rumen Science 2011 331 6016 463 467 10.1126/science.1200387 21273488 · DOI · Paper page
  27. [29]Hu D Chao Y Li Y Peng X Wang C Wang Z Zhang D Li K Effect of gender bias on equine fecal microbiota J Equine Vet Sci 2021 97 103355 10.1016/j.jevs.2020.103355 33478764 · DOI
  28. [31]Canani RB Costanzo MD Leone L Pedata M Meli R Calignano A Potential beneficial effects of butyrate in intestinal and extraintestinal diseases World J Gastroenterol 2011 17 12 1519 1528 10.3748/wjg.v17.i12.1519 21472114 PMC3070119 · DOI · Paper page
  29. [32]Liu H Xu T Xu S Effect of dietary concentrate to forage ratio on growth performance, rumen fermentation and bacterial diversity of Tibetan sheep under barn feeding on the Qinghai-Tibetan plateau PeerJ 2019 7 e7462 10.7717/peerj.7462 31404417 PMC6686838 · DOI
  30. [33]Pan X Xue F Nan X Tang Z Wang K Beckers Y Jiang L Xiong B Illumina sequencing approach to characterize thiamine metabolism related bacteria and the impacts of thiamine supplementation on ruminal microbiota in dairy cows fed high-grain diets Front Microbiol 2017 8 1818 10.3389/fmicb.2017.01818 28979254 PMC5611408 · DOI
  31. [34]Suen G Stevenson DM Bruce DC Chertkov O Copeland A Cheng JF Complete genome of the cellulolytic ruminal bacterium Ruminococcus albus 7 J Bacteriol 2011 193 19 5574 5575 10.1128/JB.05621-11 21914885 PMC3187470 · DOI
  32. [35]Arntzen MØ Várnai A Mackie RI Eijsink VGH Pope PB Outer membrane vesicles from Fibrobacter succinogenes S85 contain an array of carbohydrate-active enzymes with versatile polysaccharide-degrading capacity Environ Microbiol 2017 19 7 2701 2714 10.1111/1462-2920.13770 28447389 · DOI · Paper page
  33. [36]Romero-Huelva M Ramos-Morales E Molina-Alcaide E Nutrient utilization, ruminal fermentation, microbial abundances, and milk yield and composition in dairy goats fed diets including tomato and cucumber waste fruits J Dairy Sci 2012 95 10 6015 6026 10.3168/jds.2012-5573 22901467 · DOI
  34. [37]Fontes FL Pinheiro DM Oliveira AH Oliveira RK Lajus TB Agnez-Lima LF Role of DNA repair in host immune response and inflammation Mutat Res Rev Mutat Res 2015 763 246 257 10.1016/j.mrrev.2014.11.004 25795123 · DOI
  35. [38]Pacífico C Petri RM Ricci S Mickdam E Wetzels SU Neubauer V Zebeli Q Unveiling the bovine epimural microbiota composition and putative function Microorganisms 2021 9 2 342 10.3390/microorganisms9020342 33572291 PMC7915655 · DOI
  36. [39]Pan JH Kim JH Kim HM Lee ES Shin DH Kim S Shin M Kim SH Lee JH Kim YJ Acetic acid enhances endurance capacity of exercise-trained mice by increasing skeletal muscle oxidative properties Biosci Biotechnol Biochem 2015 79 9 1535 1541 10.1080/09168451.2015.1034652 26000971 · DOI
  37. [40]Des Rosiers C David F Garneau M Brunengraber H Nonhomogeneous labeling of liver mitochondrial acetyl-CoA J Biol Chem 1991 266 3 1574 1578 10.1016/S0021-9258(18)52332-2 1988437 · DOI
  38. [41]Bilk S Huhn K Honscha KU Pfannkuche H Gäbel G Bicarbonate exporting transporters in the ovine ruminal epithelium J Comp Physiol B 2005 175 5 365 374 10.1007/s00360-005-0493-1 15926041 · DOI
  39. [42]Van Soest PJ Robertson JB Lewis BA Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition J Dairy Sci 1991 74 10 3583 3597 10.3168/jds.S0022-0302(91)78551-2 1660498 · DOI · Paper page
  40. [44]Muyzer G de Waal EC Uitterlinden AG Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA Appl Environ Microbiol 1993 59 3 695 700 10.1128/aem.59.3.695-700.1993 7683183 PMC202176 · DOI
  41. [45]Bokulich NA Subramanian S Faith JJ Gevers D Gordon JI Knight R Mills DA Caporaso JG Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing Nat Methods 2013 10 1 57 59 10.1038/nmeth.2276 23202435 PMC3531572 · DOI

Cited by

Sorted by citations

Authors
Guo, X., Sha, Y., Lv, W., Pu, X., Liu, X., Luo, Y., Hu, J., Wang, J., Li, S., & Zhao, Z.
Affiliations
Gansu Agricultural University (CN)
Corresponding author
Liu, Xiu
ORCID
Venue
Microbial Cell Factories
Publisher
Springer Science and Business Media LLC
Published
2022-04-07
Volume
21
Issue
1
Pages
55-55
ISSN
1475-2859 (ISSN-L)
DOI
10.1186/s12934-022-01783-8
PMC
PMC8991483
OpenAlex
W4225343827
Cited by
58
References
3
Citations by year
2026: 9 · 2025: 12 · 2024: 29 · 2023: 6 · 2022: 2
FWCI
16.45
Type
journal article
Language
en
Open access
Licence
Locations
  • Microbial Cell Factories (publishedVersion, cc-by)
  • Research Square (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Innovation Funds of Gansu province institution of higher learning (2020A-52)
  • Youth Mentor of Gansu Agricultural University (GAU-QDFC-2018-11)
  • National Natural Science Foundation of China (31860688)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
Keywords
sex differences, rumen fermentation, rumen microbiota, Tibetan goat, volatile fatty acids, rumen epithelium, Fibrobacter, plateau adaptation, Firmicutes, Bacteroidetes
MeSH terms
Animals, Bacteria, Female, Fermentation, Goats, Male, Rumen, Sex Characteristics, Tibet, Microbiota

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review