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Cited by 29Microbial Metabolic Engineering and BioproductionAdvanced Scientific Research MethodsAlgal biology and biofuel production

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The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions

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Sakarika, Myrsini, Kerckhof, Frederiek-Maarten, Van Peteghem, Lotte, Pereira, Alexandra, Van Den Bossche, Tim, Bouwmeester, Robbin, Gabriels, Ralf, Van Haver, Delphi, Ulčar, Barbara, Martens, Lennart, Impens, Francis, Boon, Nico, Ganigué, Ramon, Rabaey, Korneel·2023·Microbial Cell Factories
DOI 10.1186/s12934-023-02265-1PMC PMC10712164
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Abstract

Background: It is increasingly recognized that conventional food production systems are not able to meet the globally increasing protein needs, resulting in overexploitation and depletion of resources, and environmental degradation. In this context, microbial biomass has emerged as a promising sustainable protein alternative. Nevertheless, often no consideration is given on the fact that the cultivation conditions affect the composition of microbial cells, and hence their quality and nutritional value. Apart from the properties and nutritional quality of the produced microbial food (ingredient), this can also impact its sustainability. To qualitatively assess these aspects, here, we investigated the link between substrate availability, growth rate, cell composition and size of Cupriavidus necator and Komagataella phaffii . Results: Biomass with decreased nucleic acid and increased protein content was produced at low growth rates. Conversely, high rates resulted in larger cells, which could enable more efficient biomass harvesting. The proteome allocation varied across the different growth rates, with more ribosomal proteins at higher rates, which could potentially affect the techno-functional properties of the biomass. Considering the distinct amino acid profiles established for the different cellular components, variations in their abundance impacts the product quality leading to higher cysteine and phenylalanine content at low growth rates. Therefore, we hint that costly external amino acid supplementations that are often required to meet the nutritional needs could be avoided by carefully applying conditions that enable targeted growth rates. Conclusion: In summary, we demonstrate tradeoffs between nutritional quality and production rate, and we discuss the microbial biomass properties that vary according to the growth conditions.

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Metadata

Authors
Sakarika, M., Kerckhof, F.-M., Van Peteghem, L., Pereira, A., Van Den Bossche, T., Bouwmeester, R., Gabriels, R., Van Haver, D., Ulčar, B., Martens, L., Impens, F., Boon, N., Ganigué, R., & Rabaey, K.
Affiliations
Ghent University (BE)
Corresponding author
Sakarika, Myrsini
ORCID
Venue
Microbial Cell Factories
Publisher
Springer Science and Business Media LLC
Published
2023-12-11
Volume
22
Issue
1
Pages
254-254
ISSN
1475-2859 (ISSN-L)
DOI
10.1186/s12934-023-02265-1
PMC
PMC10712164
OpenAlex
W4389554120
Cited by
29
References
1
Citations by year
2026: 12 · 2025: 14 · 2024: 3
FWCI
2.58
Type
journal article
Language
en
Open access
Licence
Locations
  • Microbial Cell Factories (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Ghent University Academic Bibliography (Ghent University) (publishedVersion, cc-by)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Agentschap Innoveren en Ondernemen (CO2PERATE, HBC.2019.2601, HBC.2018.0188, HBC.2020.2205)
  • Fonds Wetenschappelijk Onderzoek (1286824N, 1S27821N)
  • Universiteit Gent (BOF19/STA/044)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Keywords
microbial biomass, nutritional composition, cell size, growth rate, Cupriavidus necator, Pichia pastoris, proteome allocation, ribosomal proteins, amino acid composition, nucleic acid content, protein content, biomass harvesting, substrate availability, techno-functional properties
MeSH terms
Amino Acids, Cysteine, Biomass, Proteome, Cell Size

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